Physical Sciences and Mathematics Commons^™

Structural And Kinetic Studies Of A Cisplatin-Modified Dna Icosamer Binding To Hmg1 Domain B*, Elizabeth R. Jamieson, Matthew P. Jacobson, Carmen M. Barnes, Christine S. Chow, Stephen J. Lippard

Chemistry: Faculty Publications

The high mobility group (HMG) domain is a DNA-binding motif found in the non-histone chromosomal proteins, HMG1 and HMG2, and some transcription factors. Experimental evidence has demonstrated that HMG-domain proteins can play a role in sensitizing cells to the anticancer drug cisplatin. Fluorescence resonance energy transfer (FRET) experiments were performed in the present study to investigate structural changes that accompany complex formation between the HMG domain B of HMG1 and a cisplatin-modified, 20-base pair double-stranded DNA probe containing fluorescein and rhodamine tethered at its two ends. The binding affinity of HMG1 domain B for the cisplatin-modified DNA probe was investigated …