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Full-Text Articles in Physical Sciences and Mathematics
Electrochemical Glucose Sensors—Developments Using Electrostatic Assembly And Carbon Nanotubes For Biosensor Construction, Alice Harper, Mark Anderson
Electrochemical Glucose Sensors—Developments Using Electrostatic Assembly And Carbon Nanotubes For Biosensor Construction, Alice Harper, Mark Anderson
Mark R. Anderson
In 1962, Clark and Lyons proposed incorporating the enzyme glucose oxidase in the construction of an electrochemical sensor for glucose in blood plasma. In their application, Clark and Lyons describe an electrode in which a membrane permeable to glucose traps a small volume of solution containing the enzyme adjacent to a pH electrode, and the presence of glucose is detected by the change in the electrode potential that occurs when glucose reacts with the enzyme in this volume of solution. Although described nearly 50 years ago, this seminal development provides the general structure for constructing electrochemical glucose sensors that is …
Electrostatic Assembly Of A Redox Catalysis System For Detection Of Glutamate, Alice Harper, Mark Anderson
Electrostatic Assembly Of A Redox Catalysis System For Detection Of Glutamate, Alice Harper, Mark Anderson
Mark R. Anderson
Interfacial assemblies capable of determining glutamate by redox catalysis are prepared by electrostatic assembly of alternating layers of ferrocene poly(allylamine) polymer and glutamate oxidase on a gold electrode. Deposition of the polymer was confirmed in cyclic voltammetry measurements by the presence of a surface wave corresponding to the oxidation of the ferrocene group. In the presence of glutamate in the adjacent electrolyte solution, the current increases and approaches a pseudosteady state, consistent with redox catalysis. Electrodes modified with glutamate oxidase had a linear response to glutamate up to 0.0045 M with sensitivity of 20 μA/cm2 and a limit of detection …