Physical Sciences and Mathematics Commons^™

Identifying Differentially Expressed Genes In Unreplicated Multiple-Treatment Microarray Timecourse Experiments, Rhonda Decook, Dan Nettleton, Carol Foster, Eve S. Wurtele

Dan Nettleton

Microarray technology has become widespread as a means to investigate gene function and metabolic pathways in an organism. A common experiment involves probing, at each of several time points, the gene expression of experimental units subjected to different treatments. Due to the high cost of microarrays, such experiments may be performed without replication and therefore provide a gene expression measurement of only one experimental unit for each combination of treatment and time point. Though an experiment with replication would provide more powerful conclusions, it is still possible to identify differentially expressed genes and to estimate the number of false positives …