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Physical Sciences and Mathematics Commons™
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Full-Text Articles in Physical Sciences and Mathematics
Methodological Approach To The Ex Vivo Expansion And Detection Of T. Cruzi-Specific T Cells From Chronic Chagas Disease Patients, Gonzalo R. Acevedo, Silvia A. Longhi, Alcinette Bunying, Nazila Sabri, Augusto Atienza, Maria P. Zago, Radleigh Santos, Valeria A. Judkowski, Clemencia Pinilla, Karina A. Gomez
Methodological Approach To The Ex Vivo Expansion And Detection Of T. Cruzi-Specific T Cells From Chronic Chagas Disease Patients, Gonzalo R. Acevedo, Silvia A. Longhi, Alcinette Bunying, Nazila Sabri, Augusto Atienza, Maria P. Zago, Radleigh Santos, Valeria A. Judkowski, Clemencia Pinilla, Karina A. Gomez
Mathematics Faculty Articles
The discovery of T cell epitopes is essential not only for gaining knowledge about host response to infectious disease but also for the development of immune-intervention strategies. In Chagas disease, given the size and complexity of the Trypanosoma cruzi proteome and its interaction with the host’s immune system, the fine specificity of T cells has not been extensively studied yet, and this is particularly true for the CD4+ T cell compartment. The aim of the present work was to optimize a protocol for the generation of parasite-specific memory T cell lines, representative of their in vivo precursor populations and capable …
Calcium Transient Assays For Compound Screening With Human Ipsc-Derived Cardiomyocytes: Evaluating New Tools, Neil J. Daily, Radleigh Santos, Joseph Vecchi, Pinar Kemanli, Tetsuro Wakatsuki
Calcium Transient Assays For Compound Screening With Human Ipsc-Derived Cardiomyocytes: Evaluating New Tools, Neil J. Daily, Radleigh Santos, Joseph Vecchi, Pinar Kemanli, Tetsuro Wakatsuki
Mathematics Faculty Articles
Calcium (Ca2+) plays a central role in regulating many biological processes in the cell from muscle contraction to neurotransmitter release. The need for reliable fluorescent calcium indicator dyes is of vast importance for studying many aspects of cell biology as well as screening compounds using phenotypic high throughput assays. We have assessed two of the latest generation of calcium indicator dyes, FLIPR Calcium 6 and Cal-520 AM for studying calcium transients (CaTs) in induced pluripotent stem cell (iPSC) -derived human cardiomyocytes. FLIPR Calcium 6 and Cal-520 dyes both displayed robust CaTs with a high signal-to-noise ratio (SNR) and were non-toxic …
Spectral Properties Of A Sequence Of Matrices Connected To Each Other Via Schur Complement And Arising In A Compartmental Model, Evan Haskell, Vehbi Emrah Paksoy
Spectral Properties Of A Sequence Of Matrices Connected To Each Other Via Schur Complement And Arising In A Compartmental Model, Evan Haskell, Vehbi Emrah Paksoy
Mathematics Faculty Articles
We consider a sequence of real matrices An which is characterized by the rule that An−1 is the Schur complement in An of the (1,1) entry of An, namely −en, where en is a positive real number. This sequence is closely related to linear compartmental ordinary differential equations. We study the spectrum of An. In particular,we show that An has a unique positive eigenvalue λn and {λn} is a decreasing convergent sequence. We also study the stability of An for small n using the Routh-Hurwitz criterion.