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Physical Sciences and Mathematics Commons™
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Full-Text Articles in Physical Sciences and Mathematics
On-Line Laser Desorption/Ionization Mass Spectrometry, Damien A. Narcisse
On-Line Laser Desorption/Ionization Mass Spectrometry, Damien A. Narcisse
LSU Doctoral Dissertations
Interfaces for on-line laser desorption/ionization mass spectrometry are presented in this dissertation. An on line laser desorption interface allows samples to be introduced directly into the mass spectrometer for high-throughput applications. For this research, a linear time of flight mass spectrometer was constructed with an ionization source designed to accept various laser desorption interfaces. A ball inlet interface was used for continuous analyte deposition at atmospheric pressure and vacuum ionization. A solvent-based cleaning system and a separate capillary for MALDI matrix delivery was utilized for continuous on line sampling with the ball inlet interface. Microfluidic devices were brought into contact …
Capillary Electrophoretic Separations Of Enzyme Inhibitors With Activity-Based Detection, Xiaoyan Yan
Capillary Electrophoretic Separations Of Enzyme Inhibitors With Activity-Based Detection, Xiaoyan Yan
LSU Master's Theses
A technique for separating and detecting enzyme inhibitors was developed using capillary electrophoresis with an enzyme microreactor. The on-column enzyme microreactor was constructed using one or two NdFeB magnets in two configurations to immobilize alkaline phosphatase-coated superparamagnetic beads with diameters of 2.8 µm inside a capillary before the detection window. Enzyme inhibition assays were performed by injecting a plug of inhibitor into a capillary filled with an alkaline phosphatase substrate, AttoPhos. Product generated in the enzyme microreactor was detected by laser-induced fluorescence. Inhibitor zones electrophoresed through the capillary, passed through the enzyme microreactor, and were observed as negative peaks due …