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Full-Text Articles in Physical Sciences and Mathematics
Ndp Kinase Moves Into Developing Primary Cilia, Kimberly A. P. Mitchell, Betty C. Gallagher, Gabor Szabo, Angela De S. Otero
Ndp Kinase Moves Into Developing Primary Cilia, Kimberly A. P. Mitchell, Betty C. Gallagher, Gabor Szabo, Angela De S. Otero
Faculty Publications and Presentations
Inmunofluorescence staining of murine NIH3T3 fibroblasts grown at high density shows that conventional nucleoside diphosphate (NDP) kinases A and B localize to a sensory organelle, the primary cilium. Similar results are obtained with Xenopus A6 kidney epithelial cells, suggesting that NDP kinases are a universal component of the primary cilium. The translocation of NDP kinase into primary cilia depends on size, taking place only when cilia reach a critical length of 5-6 μm. In mature cilia, NDP kinases are distributed along the ciliary shaft in a punctate pattern that is distinct from the continuous staining observed with acetylated α-tubulin, a …
Receptor Activation Regulates Cortical, But Not Vesicular Localization Of Ndp Kinase, Betty C. Gallagher, Kimberly A.P. Mitchell, Gabor Szabo, Angela De S. Otero
Receptor Activation Regulates Cortical, But Not Vesicular Localization Of Ndp Kinase, Betty C. Gallagher, Kimberly A.P. Mitchell, Gabor Szabo, Angela De S. Otero
Faculty Publications and Presentations
We used immunofluorescence techniques to determine the localization of nucleoside diphosphate (NDP) kinase in NIH-3T3 fibroblasts. We found that cytoplasmic NDP kinase can be separated into two populations according to subcellular localization and response to extracellular stimuli. Specifically, within minutes of stimulation of resting fibroblasts with serum, growth factors or bombesin, a portion of NDP kinase becomes associated with membrane ruffles and lamellipodia. Another pool of NDP kinase accumulates independently of stimulation around intracellular vesicles. Transfection of cells with activated Rac mimics, whereas expression of dominant negative Rac inhibits, the effects of extracellular stimulation on the translocation of NDP kinase …