Physical Sciences and Mathematics Commons^™

Epr, Endor And Dft Studies On X-Irradiated Single Crystals Of L-Lysine Monohydrochloride Monohydrate And L-Arginine Monohydrocloride Monohydrate, Yiying Zhou

Physics and Astronomy Dissertations

When proteins and DNA interact, arginine and lysine are the two amino acids most often in close contact with the DNA. In order to understand the radiation damage to DNA in vivo, which is always associated with protein, it is important to learn the radiation chemistry of arginine and lysine independently, and when complexed to DNA. This work studied X-irradiated single crystals of L-lysine monohydrochloride dihydrate (L-lysine·HCl·2H2O) and L-arginine monohydrochloride monohydrate (L-arginine·HCl·H2O) with EPR, ENDOR, EIE techniques and DFT calculations. In both crystal types irradiated at 66K, the carboxyl anion radical and the decarboxylation radical were detected. DFT calculations supported …