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Full-Text Articles in Physical Sciences and Mathematics
Design And Characterization Of A Synthetic Electron-Transfer Protein, Anna Y. Kornilova, James F. Wishart, Wenzhong Xiao, Robin C. Lasey, Anna Fedorova, Yeon-Kyun Shin, Michael Y. Ogawa
Design And Characterization Of A Synthetic Electron-Transfer Protein, Anna Y. Kornilova, James F. Wishart, Wenzhong Xiao, Robin C. Lasey, Anna Fedorova, Yeon-Kyun Shin, Michael Y. Ogawa
Chemistry Faculty Publications
A 30-residue polypeptide [H21(30-mer)] with the sequence Ac-K(IEALEGK)(2)(IEALEHK)-(IEALEGK)G-NH2 was synthesized. The circular dichroism (CD) spectrum of the peptide shows minima at 208 and 222 nm and theta(222)/theta(208) = 1.06, which indicates the formation of a self-assembled coiled-coil when dissolved in aqueous solution. The concentration dependence of the CD data can be fit to an expression that describes a two-state monomer-dimer equilibrium for the apopeptide (K-d = 1.5 +/- 0.4 mu M and theta(max) = -23 800 +/- 130 deg cm(2) dmol(-1)), showing that it has a maximum helicity of 69%. A [MTSL-C21(30-mer)] dimer was also prepared in which MTSL is …
Spectroscopy Of Hydrogen-Bonded Formanilide Clusters In A Supersonic Jet: Solvation Of A Model Trans Amide, A. V. Fedorov, John R. Cable
Spectroscopy Of Hydrogen-Bonded Formanilide Clusters In A Supersonic Jet: Solvation Of A Model Trans Amide, A. V. Fedorov, John R. Cable
Chemistry Faculty Publications
The gas-phase structures of trans-formanilide (FA) clusters containing varying numbers of water and ammonia molecules have been investigated by resonant two-photon ionization spectroscopy in a supersonic jet expansion. A single structure is found for the 1:1 cluster of FA with ammonia in which the amide NH group functions as a hydrogen bond donor to the ammonia nitrogen. In contrast, vibronically resolved spectra reveal two distinct structures for the 1:1 cluster with water in which either the amide NH group functions as a hydrogen bond donor or the carbonyl oxygen functions as a hydrogen bond acceptor. The 1:1 clusters with both …