Open Access. Powered by Scholars. Published by Universities.®
Physical Sciences and Mathematics Commons™
Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Physical Sciences and Mathematics
The Photoreduction Of Indigo Carmine Flavin Mononucleotide And Diphosphopyridine Nucleotide, By Illuminated Chloroplasts, M. Odell Hobbs
The Photoreduction Of Indigo Carmine Flavin Mononucleotide And Diphosphopyridine Nucleotide, By Illuminated Chloroplasts, M. Odell Hobbs
Theses and Dissertations
An investigation was made of the photoreduction of indigo carmine dye and flavin mononucleotide (FMN) by an illuminated sugar beet leaf homogenate in the presence of 2,6-dichlorophenolindophenol dye (DPIP) and ascorbic acid. DPIP and ascorbate were absolutely required for the reaction. Reconstitution experiments indicated a requirement for a heat-labile factor present in the supernatant fluid following centrifugation of the leaf homogenate. This factor activated the photoreduction of indigo carmine more than 20 times. The photoreduction of FMN was activated 2 times by this factor. Studies on the stoichiometry of indigo carmine photoreduction revealed that one mole of ascorbate was photooxidized …