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Physical Sciences and Mathematics Commons

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Faculty Publications

1996

Chemistry

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Full-Text Articles in Physical Sciences and Mathematics

The Advanced Glycation End Product, NE-(Carboxymethyl)Lysine, Is A Product Of Both Lipid Peroxidation And Glycoxidation Reactions, Min-Xin Fu, Jesus R. Requena, Alicia J. Jenkins, Timothy J. Lyons, John W. Baynes, Suzanne R. Thorpe Apr 1996

The Advanced Glycation End Product, NE-(Carboxymethyl)Lysine, Is A Product Of Both Lipid Peroxidation And Glycoxidation Reactions, Min-Xin Fu, Jesus R. Requena, Alicia J. Jenkins, Timothy J. Lyons, John W. Baynes, Suzanne R. Thorpe

Faculty Publications

Nepsilon-(Carboxymethyl)lysine (CML) is an advanced glycation end product formed on protein by combined nonenzymatic glycation and oxidation (glycoxidation) reactions. We now report that CML is also formed during metal-catalyzed oxidation of polyunsaturated fatty acids in the presence of protein. During copper-catalyzed oxidation in vitro, the CML content of low density lipoprotein increased in concert with conjugated dienes but was independent of the presence of the Amadori compound, fructoselysine, on the protein. CML was also formed in a time-dependent manner in RNase incubated under aerobic conditions in phosphate buffer containing arachidonate or linoleate; only trace amounts of CML were formed from …