Physical Sciences and Mathematics Commons^™

[3H]‑Raffinose, A Novel Radioactive Label For Determining Organ Sites Of Catabolism Of Proteins In The Circulation, Jonathon Van Zile, Lee A. Henderson, John W. Baynes, Suzanne R. Thorpe

Faculty Publications

The primary tissue sites of catabolism of plasma proteins with long circulating half-lives are unknown. It has been difficult to identify these sites because plasma proteins are delivered to tissues at relatively slow rates but are rapidly degraded intracellularly within lysosomes. Therefore, a tracer attached to protein is lost from the site of uptake before an amount sufficient for quantitation can accumulate. We hypothesized that sucrose (Glupal-2 /3Fruf) would be a useful label to circumvent this difficulty because of the stability of sucrose in lysosomes; and thus, sucrose should remain in tissue long after the protein to which it was …