Physical Sciences and Mathematics Commons^™

A Column Free Protein Purification Procedure Using E. Coli Single ‐ Stranded Dna Binding Protein (Ssb) As An Affinity Tag, Mark Soffe

Research on the Hill (Salt Lake City)

SSBs are DNA binding proteins that are essential components of cells and play key roles in DNA replication, repair, and recombination. Here we utilize two biochemical properties associated with the E. coli SSB protein to develop a novel procedure to purify proteins using a resin-free strategy to combat the largest bottleneck in biochemical research— obtaining uncontaminated, single proteins from the total cellular contents.

1. E. coli SSB binds to single stranded DNA (ssDNA) with extremely high affinity.

2. It is also a unique protein with respect to its purification—it is possible to obtain greater than 95% pure SSB from the …

Analysis Of Mexican Spotted Owl Diet In The Canyonlands Of Southern Utah, Natalie Schvaneveldt

Research on the Hill (Salt Lake City)

While diets of Mexican Spotted Owls within forested habitat have been studied, little research has been published on the diet of owls that occupy canyon habitats (see Willey In Press). Since the Mexican Spotted Owl is federally listed as a threatened species, it is important to identify primary prey of Utah’s canyon dwelling owls to better understand their dietary needs (U.S. Fish and Wildlife Service 1993). We hope that the findings from this research can better inform state and federal managers on spotted owl prey use and aid in future management of small mammal populations in canyon habitats. We intend …

Facile Preparation Of 1st-­‐Row Transition Metal Chalcogenides As Hydrogen Evolution Catalysts In Water, Lia Bogoev

Research on the Hill (Salt Lake City)

Hydrogen, when generated directly from water with renewal energy input, is a promising chemical fuel for sustainable energy applications. As such, the development of synthetic catalysts of hydrogen evolution reaction (HER) that functionally mimic the elegant water reduction chemistry of hydrogenase enzymes have attracted broad interest, but creating molecular systems that utilize earth-abundant elements and achieve high activity, low overpotential, and long-term stability in green aqueous media remains a significant challenge. Solid-state inorganic compounds offer an alternative strategy to meet this goal. Our group is developing 1st-row transition metal chalcogenides via facile potential dynamic deposition as competent HER catalysts in …