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Full-Text Articles in Physical Sciences and Mathematics

Character Amenability Of Vector-Valued Algebras, Terje Hill, David Robbins Dec 2023

Character Amenability Of Vector-Valued Algebras, Terje Hill, David Robbins

Faculty Scholarship

Let {Ax:x∈X} be a collection of complex Banach algebras indexed by the compact Hausdorff space X. We investigate the character amenability of certain algebras A of Ax-valued functions in relation to the character amenability of the Ax.


Probability Distribution Of Sars-Cov-2 (Covid) Infectivity Following Onset Of Symptoms: Analysis From First Principles, Mark P. Silverman Jan 2023

Probability Distribution Of Sars-Cov-2 (Covid) Infectivity Following Onset Of Symptoms: Analysis From First Principles, Mark P. Silverman

Faculty Scholarship

The phasing out of protective measures by governments and public health agencies, despite continued seriousness of the coronavirus pandemic, leaves individuals who are concerned for their health with two basic options over which they have control: 1) minimize risk of infection by being vaccinated and by wearing a face mask when appropriate, and 2) minimize risk of transmission upon infection by self-isolating. For the latter to be effective, it is essential to have an accurate sense of the probability of infectivity as a function of time following the onset of symptoms. Epidemiological considerations suggest that the period of infectivity follows …


Triarylmethyl Cation-Catalyzed Three-Component Coupling For The Synthesis Of Unsymmetrical Bisindolylmethanes [Post-Print], William Patterson, Kelly Lucas, Vanessa Jones, Zhenghua Chen, Kevin Bardelski, Melissa Guarino-Hotz, Cheyenne S. Brindle Nov 2021

Triarylmethyl Cation-Catalyzed Three-Component Coupling For The Synthesis Of Unsymmetrical Bisindolylmethanes [Post-Print], William Patterson, Kelly Lucas, Vanessa Jones, Zhenghua Chen, Kevin Bardelski, Melissa Guarino-Hotz, Cheyenne S. Brindle

Faculty Scholarship

An efficient synthesis of unsymmetrical bisindolylmethanes has been accomplished using triarylmethyl cations to catalyze the reaction of N-arylimines with two different indoles. Optimization of the organocatalyst by tuning cation stability allows for excellent single addition selectivity when coupled with p-nitrophenyl imines. The optimal catalyst is commercially available, and the reaction minimizes waste and environmental impact by employing a one-to-one ratio of starting materials. The intermediates can be isolated or used insitu in a one-pot two-step reaction to generate unsymmetrical bisindolylmethanes in high yields. The reaction tolerates a broad range of imines with the highest yields observed for …


Supported Bilayer Membranes For Reducing Cell Adhesion In Microfluidic Devices [Post-Print], Julia R. Clapis, Mengqi Jonathan Fan, Michelle Kovarik Mar 2021

Supported Bilayer Membranes For Reducing Cell Adhesion In Microfluidic Devices [Post-Print], Julia R. Clapis, Mengqi Jonathan Fan, Michelle Kovarik

Faculty Scholarship

The high surface area-to-volume ratio of microfluidic channels makes them susceptible to fouling and clogging when used for biological analyses, including cell-based assays. We evaluated the role of electrostatic and van der Waals interactions in cell adhesion in PDMS microchannels coated with supported lipid bilayers and identified conditions that resulted in minimal cell adhesion. For low ionic strength buffer, optimum results were obtained for a zwitterionic coating of pure egg phosphatidylcholine; for a rich growth medium, the best results were obtained for zwitterionic bilayers or those with slight negative or moderate positive charge from the incorporation of 5-10 mol% egg …


Higher Order Interactions In Complex Networks Of Phase Oscillators Promote Abrupt Synchronization Switching, Per Sebastian Skardal, Alex Arenas Dec 2020

Higher Order Interactions In Complex Networks Of Phase Oscillators Promote Abrupt Synchronization Switching, Per Sebastian Skardal, Alex Arenas

Faculty Scholarship

© 2020, The Author(s). Synchronization processes play critical roles in the functionality of a wide range of both natural and man-made systems. Recent work in physics and neuroscience highlights the importance of higher-order interactions between dynamical units, i.e., three- and four-way interactions in addition to pairwise interactions, and their role in shaping collective behavior. Here we show that higher-order interactions between coupled phase oscillators, encoded microscopically in a simplicial complex, give rise to added nonlinearity in the macroscopic system dynamics that induces abrupt synchronization transitions via hysteresis and bistability of synchronized and incoherent states. Moreover, these higher-order interactions can stabilize …


Diversity Patterns Associated With Varying Dispersal Capabilities As A Function Of Spatial And Local Environmental Variables In Small Wetlands In Forested Ecosystems, Brett M. Tornwall, Amber L. Pitt, Bryan L. Brown, Joanna Hawley-Howard, Robert F. Baldwin Nov 2020

Diversity Patterns Associated With Varying Dispersal Capabilities As A Function Of Spatial And Local Environmental Variables In Small Wetlands In Forested Ecosystems, Brett M. Tornwall, Amber L. Pitt, Bryan L. Brown, Joanna Hawley-Howard, Robert F. Baldwin

Faculty Scholarship

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. The diversity of species on a landscape is a function of the relative contribution of diversity at local sites and species turnover between sites. Diversity partitioning refers to the relative contributions of alpha (local) and beta (species turnover) diversity to gamma (regional/landscape) diversity and can be influenced by the relationship between dispersal capability as well as spatial and local environmental variables. Ecological theory predicts that variation in the distribution of organisms that are strong dispersers will be less influenced by spatial properties such as topography and connectivity of a region and …


Review Of Student-Built Spectroscopy Instrumentation Projects, Michelle L. Kovarik, Julia R. Clapis, K. Ana Romano-Pringle Aug 2020

Review Of Student-Built Spectroscopy Instrumentation Projects, Michelle L. Kovarik, Julia R. Clapis, K. Ana Romano-Pringle

Faculty Scholarship

Copyright © 2020 American Chemical Society and Division of Chemical Education, Inc. One challenge of teaching chemical analysis is the proliferation of sophisticated, but often impenetrable, instrumentation in the modern laboratory. Complex instruments, and the software that runs them, distance students from the physical and chemical processes that generate the analytical signal. A solution to this challenge is the introduction of a student-driven instrument-building project. Visible absorbance spectroscopy is well-suited to such a project due to its relative simplicity and the ubiquity of absorbance measurements. This Article reviews simple instructor- A nd student-built instruments for spectroscopy, providing an overview of …


Extraction Of Information From Crowdsourcing: Experimental Test Employing Bayesian, Maximum Likelihood, And Maximum Entropy Methods, Mark P. Silverman Oct 2019

Extraction Of Information From Crowdsourcing: Experimental Test Employing Bayesian, Maximum Likelihood, And Maximum Entropy Methods, Mark P. Silverman

Faculty Scholarship

A crowdsourcing experiment in which viewers (the “crowd”) of a British Broadcasting Corporation (BBC) television show submitted estimates of the number of coins in a tumbler was shown in an antecedent paper (Part 1) to follow a log-normal distribution ∧(m,s2). The coin-estimation experiment is an archetype of a broad class of image analysis and object counting problems suitable for solution by crowdsourcing. The objective of the current paper (Part 2) is to determine the location and scale parameters (m,s) of ∧(m,s2) by both Bayesian and maximum …


Model Reconstruction From Temporal Data For Coupled Oscillator Networks, Mark J. Panaggio, Maria Veronica Ciocanel, Lauren Lazarus, Chad M. Topaz, Bin Xu Oct 2019

Model Reconstruction From Temporal Data For Coupled Oscillator Networks, Mark J. Panaggio, Maria Veronica Ciocanel, Lauren Lazarus, Chad M. Topaz, Bin Xu

Faculty Scholarship

© 2019 Author(s). In a complex system, the interactions between individual agents often lead to emergent collective behavior such as spontaneous synchronization, swarming, and pattern formation. Beyond the intrinsic properties of the agents, the topology of the network of interactions can have a dramatic influence over the dynamics. In many studies, researchers start with a specific model for both the intrinsic dynamics of each agent and the interaction network and attempt to learn about the dynamics of the model. Here, we consider the inverse problem: given data from a system, can one learn about the model and the underlying network? …


Crowdsourced Sampling Of A Composite Random Variable: Analysis, Simulation, And Experimental Test, Mark P. Silverman Aug 2019

Crowdsourced Sampling Of A Composite Random Variable: Analysis, Simulation, And Experimental Test, Mark P. Silverman

Faculty Scholarship

A composite random variable is a product (or sum of products) of statistically distributed quantities. Such a variable can represent the solution to a multi-factor quantitative problem submitted to a large, diverse, independent, anonymous group of non-expert respondents (the “crowd”). The objective of this research is to examine the statistical distribution of solutions from a large crowd to a quantitative problem involving image analysis and object counting. Theoretical analysis by the author, covering a range of conditions and types of factor variables, predicts that composite random variables are distributed log-normally to an excellent approximation. If the factors in a problem …


Progressive Randomization Of A Deck Of Playing Cards: Experimental Tests And Statistical Analysis Of The Riffle Shuffle, Mark P. Silverman Apr 2019

Progressive Randomization Of A Deck Of Playing Cards: Experimental Tests And Statistical Analysis Of The Riffle Shuffle, Mark P. Silverman

Faculty Scholarship

The question of how many shuffles are required to randomize an initially ordered deck of cards is a problem that has fascinated mathematicians, scientists, and the general public. The two principal theoretical approaches to the problem, which differed in how each defined randomness, has led to statistically different threshold numbers of shuffles. This paper reports a comprehensive experimental analysis of the card randomization problem for the purposes of determining 1) which of the two theoretical approaches made the more accurate prediction, 2) whether different statistical tests yield different threshold numbers of randomizing shuffles, and 3) whether manual or mechanical shuffling …


Collaborative Learning Exercises For Teaching Protein Mass Spectrometry [Post-Print], Michelle Kovarik, Jill K. Robinson Mar 2019

Collaborative Learning Exercises For Teaching Protein Mass Spectrometry [Post-Print], Michelle Kovarik, Jill K. Robinson

Faculty Scholarship

A collaborative learning module for teaching protein mass spectrometry has been developed to overcome common obstacles to incorporating the modern topic of biological mass spectrometry into the undergraduate chemistry curriculum. Protein mass spectrometry data is provided to eliminate the need for expensive instrumentation, and an instructor’s manual gives necessary details for those unfamiliar with the topic. The first section provides background information on proteins and the field of proteomics. The second section describes the use of electrospray ionization to determine the molecular weight of a protein. The third section shows how to identify a protein using peptide mass mapping, and …


Student Engagement Is Key To Broadening Participation In Cs, Beryl Hoffman, Ralph Morelli, Jennifer Rosato Feb 2019

Student Engagement Is Key To Broadening Participation In Cs, Beryl Hoffman, Ralph Morelli, Jennifer Rosato

Faculty Scholarship

© 2019 Copyright held by the owner/author(s). Publication rights licensed to ACM. The Mobile CS Principles (Mobile CSP) course is one of the NSF-supported, College Board-endorsed curricula for the new Computer Science Principles AP course. Since 2013, the Mobile CSP project has trained more than 700 teachers, and the course has been offered to more than 20,000 students throughout the United States. The organizing philosophy behind the Mobile CSP course is that student engagement in the classroom is the key to getting students, especially those traditionally underrepresented in CS, interested in pursuing further study and careers in CS. The main …


Symmetry And Symmetry Breaking In Coupled Oscillator Communities [Post-Print], Per Sebastian Skardal Jan 2019

Symmetry And Symmetry Breaking In Coupled Oscillator Communities [Post-Print], Per Sebastian Skardal

Faculty Scholarship

With the recent development of analytical methods for studying the collective dynamics of coupled oscillator systems, the dynamics of communities of coupled oscillators have received a great deal of attention in the nonlinear dynamics community. However, the majority of these works treat systems with a number of symmetries to simplify the analysis. In this work we study the role of symmetry and symmetry-breaking in the collective dynamics of coupled oscillator communities, allowing for a comparison between the macroscopic dynamics of symmetric and asymmetric systems. We begin by treating the symmetric case, deriving the bifurcation diagram as a function of intra- …


The Role Of Friction In The Static Equilibrium Of A Fixed Ladder: Theoretical Analysis And Experimental Test, Mark P. Silverman Dec 2018

The Role Of Friction In The Static Equilibrium Of A Fixed Ladder: Theoretical Analysis And Experimental Test, Mark P. Silverman

Faculty Scholarship

In a recent publication the author derived and experimentally tested several theoretical models, distinguished by different boundary conditions at the contacts with horizontal and vertical supports, that predicted the forces of reaction on a fixed (i.e. inextensible) ladder. This problem is statically indeterminate since there are 4 forces of reaction and only 3 equations of static equilibrium. The model that predicted the empirical reactions correctly used a law of static friction to complement the equations of static equilibrium. The present paper examines in greater theoretical and experimental detail the role of friction in accounting for the forces of reaction on …


Dynamics Of Nonlinear Random Walks On Complex Networks, Per Sebastian Skardal, Sabina Adhikari Dec 2018

Dynamics Of Nonlinear Random Walks On Complex Networks, Per Sebastian Skardal, Sabina Adhikari

Faculty Scholarship

In this paper, we study the dynamics of nonlinear random walks. While typical random walks on networks consist of standard Markov chains whose static transition probabilities dictate the flow of random walkers through the network, nonlinear random walks consist of nonlinear Markov chains whose transition probabilities change in time depending on the current state of the system. This framework allows us to model more complex flows through networks that may depend on the current system state. For instance, under humanitarian or capitalistic direction, resource flow between institutions may be diverted preferentially to poorer or wealthier institutions, respectively. Importantly, the nonlinearity …


Writing In The Disciplines: How Math Fits Into The Equation [Post-Print], Madelyn W. Colonnese, Christina M. Amspaugh, Steven Lemay, Kyle Evans, Kathryn Field Nov 2018

Writing In The Disciplines: How Math Fits Into The Equation [Post-Print], Madelyn W. Colonnese, Christina M. Amspaugh, Steven Lemay, Kyle Evans, Kathryn Field

Faculty Scholarship

© 2018 International Literacy Association Writing is an important mode of thinking and learning for elementary students. Consistent efforts have been made to encourage discipline-specific writing, yet defining qualities of elementary mathematical writing have historically been underdeveloped. This article offers educators a new framework that conceptualizes mathematical writing as writing to reason and to communicate mathematically. Specifically, the framework defines four types of elementary mathematical writing: exploratory, informative/explanatory, argumentative, and mathematically creative. The authors explain and explore these types and their associated purposes through classroom vignettes. Informed by existing practices in mathematics and writing, strategies are offered to support teachers …


Effect Of Loading Method On A Peptide Substrate Reporter In Intact Cells [Post-Print], Rahuljeet Chadha, Grigorii Kalminskii, Allison Tierney, Joshua Knopf, Lorena Lazo De La Vega, Berjana Mcelrath, Michelle Kovarik Sep 2018

Effect Of Loading Method On A Peptide Substrate Reporter In Intact Cells [Post-Print], Rahuljeet Chadha, Grigorii Kalminskii, Allison Tierney, Joshua Knopf, Lorena Lazo De La Vega, Berjana Mcelrath, Michelle Kovarik

Faculty Scholarship

Studies of live cells often require loading of exogenous molecules through the cell membrane; however, effects of loading method on experimental results are poorly understood. Therefore, in this work, we compared three methods for loading a fluorescently labeled peptide into cells of the model organism Dictyostelium discoideum. We optimized loading by pinocytosis, electroporation, and myristoylation to maximize cell viability and characterized loading efficiency, localization, and uniformity. We also determined how the loading method affected measurements of enzyme activity on the peptide substrate reporter using capillary electrophoresis. Loading method had a strong effect on the stability and phosphorylation of the …


Reaction Forces On A Fixed Ladder In Static Equilibrium: Analysis And Definitive Experimental Test Of The Ladder Problem, Mark P. Silverman Sep 2018

Reaction Forces On A Fixed Ladder In Static Equilibrium: Analysis And Definitive Experimental Test Of The Ladder Problem, Mark P. Silverman

Faculty Scholarship

The development of a theoretical model to predict the four equilibrium forces of reaction on a simple ladder of non-adjustable length leaning against a wall has long remained an unresolved matter. The difficulty is that the problem is statically indeterminate and therefore requires complementary information to obtain a unique solution. This paper reports 1) a comprehensive theoretical analysis of the three fundamental models based on treating the ladder as a single Euler-Bernoulli beam, and 2) a detailed experimental investigation of the forces of reaction as a function of applied load and location of load. In contrast to previous untested proposals …


Pseudoelasticity At Large Strains In Au Nanocrystals [Post-Print], X. Wendy Gu, Lindsey A. Hanson, Carissa N. Eisler, Matthew A. Koc, A. Paul Alivisatos Aug 2018

Pseudoelasticity At Large Strains In Au Nanocrystals [Post-Print], X. Wendy Gu, Lindsey A. Hanson, Carissa N. Eisler, Matthew A. Koc, A. Paul Alivisatos

Faculty Scholarship

© 2018 American Physical Society. Pseudoelasticity in metals is typically associated with phase transformations (e.g., shape memory alloys) but has recently been observed in sub-10 nm Ag nanocrystals that rapidly recovered their original shape after deformation to large strains. The discovery of pseudoelasticity in nanoscale metals dramatically changes the current understanding of the properties of solids at the smallest length scales, and the motion of atoms at surfaces. Yet, it remains unclear whether pseudoelasticity exists in different metals and nanocrystal sizes. The challenge of observing deformation at atomistic to nanometer length scales has prevented a clear mechanistic understanding of nanoscale …


Bending Of A Tapered Rod: Modern Application And Experimental Test Of Elastica Theory, Mark P. Silverman, Joseph Farrah Jul 2018

Bending Of A Tapered Rod: Modern Application And Experimental Test Of Elastica Theory, Mark P. Silverman, Joseph Farrah

Faculty Scholarship

A tapered rod mounted at one end (base) and subject to a normal force at the other end (tip) is a fundamental structure of continuum mechanics that occurs widely at all size scales from radio towers to fishing rods to micro-electromechanical sensors. Although the bending of a uniform rod is well studied and gives rise to mathematical shapes described by elliptic integrals, no exact closed form solution to the nonlinear differential equations of static equilibrium is known for the deflection of a tapered rod. We report in this paper a comprehensive numerical analysis and experimental test of the exact theory …


Successfully Navigating The Early Years Of A Faculty Position, Michelle L. Kovarik, Christopher R. Harrison, Thomas J. Wenzel Mar 2018

Successfully Navigating The Early Years Of A Faculty Position, Michelle L. Kovarik, Christopher R. Harrison, Thomas J. Wenzel

Faculty Scholarship

No abstract provided.


Analysis Of Residence Time In The Measurement Of Radon Activity By Passive Diffusion In An Open Volume: A Micro-Statistical Approach, Mark P. Silverman Aug 2017

Analysis Of Residence Time In The Measurement Of Radon Activity By Passive Diffusion In An Open Volume: A Micro-Statistical Approach, Mark P. Silverman

Faculty Scholarship

Residence time in a flow measurement of radioactivity is the time spent by a pre-determined quantity of radioactive sample in the flow cell. In a recent report of the measurement of indoor radon by passive diffusion in an open volume (i.e. no flow cell or control volume), the concept of residence time was generalized to apply to measurement conditions with random, rather than directed, flow. The generalization, leading to a quantity r ∆t , involved use of a) a phenomenological alpha-particle range function to calculate the effective detection volume, and b) a phenomenological description of diffusion by Fick’s law to …


Triarylmethyl Cation Catalysis: A Tunable Lewis Acid Organocatalyst For The Synthesis Of Bisindolylmethanes, Nicholas G. Boekell, Dana J. Cerone, Maria M. Boucher, Wilfried B. Nganyak Tentchou, Christine G. Reavis, Ifeanyi I. Okoh, Jordan O.A. Reid, Hayley E. Berg, Briana A. Chang, Cheyenne S. Brindle Jan 2017

Triarylmethyl Cation Catalysis: A Tunable Lewis Acid Organocatalyst For The Synthesis Of Bisindolylmethanes, Nicholas G. Boekell, Dana J. Cerone, Maria M. Boucher, Wilfried B. Nganyak Tentchou, Christine G. Reavis, Ifeanyi I. Okoh, Jordan O.A. Reid, Hayley E. Berg, Briana A. Chang, Cheyenne S. Brindle

Faculty Scholarship

Triarylmethyl cations serve as tunable organocatalysts for the synthesis of bisindolylmethanes. The catalyst structure can be modified to increase or decrease reactivity as needed to match the requirements of the substrate. High yields are achieved for a variety of substrates by using these green catalysts. Catalyst tuning allows for the use of less reactive electrophiles by increasing the reactivity of the catalyst. Acid-sensitive products can be isolated under these mild reaction conditions.


Collective Phenomena Emerging From The Interactions Between Dynamical Processes In Multiplex Networks, Vincenzo Nicosia, Per Sebastian Skardal, Alex Arenas, Vito Latora Jan 2017

Collective Phenomena Emerging From The Interactions Between Dynamical Processes In Multiplex Networks, Vincenzo Nicosia, Per Sebastian Skardal, Alex Arenas, Vito Latora

Faculty Scholarship

We introduce a framework to intertwine dynamical processes of different nature, each with its own distinct network topology, using a multilayer network approach. As an example of collective phenomena emerging from the interactions of multiple dynamical processes, we study a model where neural dynamics and nutrient transport are bidirectionally coupled in such a way that the allocation of the transport process at one layer depends on the degree of synchronization at the other layer, and vice versa. We show numerically, and we prove analytically, that the multilayer coupling induces a spontaneous explosive synchronization and a heterogeneous distribution of allocations, otherwise …


Diffusion Dynamics And Synchronizability Of Hierarchical Products Of Networks, Per Sebastian Skardal Jan 2017

Diffusion Dynamics And Synchronizability Of Hierarchical Products Of Networks, Per Sebastian Skardal

Faculty Scholarship

The hierarchical product of networks represents a natural tool for building large networks out of two smaller subnetworks: a primary subnetwork and a secondary subnetwork. Here we study the dynamics of diffusion and synchronization processes on hierarchical products. We apply techniques previously used for approximating the eigenvalues of the adjacency matrix to the Laplacian matrix, allowing us to quantify the effects that the primary and secondary subnetworks have on diffusion and synchronization in terms of a coupling parameter that weighs the secondary subnetwork relative to the primary subnetwork. Diffusion processes are separated into two regimes: for small coupling the diffusion …


Scientific Controversies: Authentic And Contrived [Post-Print], Mark P. Silverman Jan 2017

Scientific Controversies: Authentic And Contrived [Post-Print], Mark P. Silverman

Faculty Scholarship

No abstract provided.


Evolving Network Structure Of Academic Institutions, Shufan Wang, Mariam Avagyan, Per Sebastian Skardal Jan 2017

Evolving Network Structure Of Academic Institutions, Shufan Wang, Mariam Avagyan, Per Sebastian Skardal

Faculty Scholarship

Today’s colleges and universities consist of highly complex structures that dictate interactions between the administration, faculty, and student body. These structures can play a role in dictating the efficiency of policy enacted by the administration and determine the effect that curriculum changes in one department have on other departments. Despite the fact that the features of these complex structures have a strong impact on the institutions, they remain by-and-large unknown in many cases. In this paper we study the academic structure of our home institution of Trinity College in Hartford, CT using the major and minor patterns between graduating students …


Spectral Properties Of The Hierarchical Product Of Graphs, Per Sebastian Skardal, Kirsti Wash Nov 2016

Spectral Properties Of The Hierarchical Product Of Graphs, Per Sebastian Skardal, Kirsti Wash

Faculty Scholarship

The hierarchical product of two graphs represents a natural way to build a larger graph out of two smaller graphs with less regular and therefore more heterogeneous structure than the Cartesian product. Here we study the eigenvalue spectrum of the adjacency matrix of the hierarchical product of two graphs. Introducing a coupling parameter describing the relative contribution of each of the two smaller graphs, we perform an asymptotic analysis for the full spectrum of eigenvalues of the adjacency matrix of the hierarchical product. Specifically, we derive the exact limit points for each eigenvalue in the limits of small and large …


Interspecies Comparison Of Peptide Substrate Reporter Metabolism Using Compartment-Based Modeling [Post-Print], Allison Tierney, Nhat Pham, Kunwei Yang, Brooks Emerick, Michelle Kovarik Nov 2016

Interspecies Comparison Of Peptide Substrate Reporter Metabolism Using Compartment-Based Modeling [Post-Print], Allison Tierney, Nhat Pham, Kunwei Yang, Brooks Emerick, Michelle Kovarik

Faculty Scholarship

Peptide substrate reporters are fluorescently labeled peptides that can be acted upon by one or more enzymes of interest. Peptide substrates are readily synthesized and more easily separated than full-length protein substrates; however, they are often more rapidly degraded by peptidases. As a result, peptide reporters must be made resistant to proteolysis in order to study enzymes in intact cells and lysates. This is typically achieved by optimizing the reporter sequence in a single cell type or model organism, but studies of reporter stability in a variety of organisms are needed to establish the robustness and broader utility of these …