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Full-Text Articles in Physical Sciences and Mathematics
Alteration Of Condensed Tannin Sythesis In Transgenic Forage Legumes, F Paolocci, G Tanner, S Arcioni, F Damiani
Alteration Of Condensed Tannin Sythesis In Transgenic Forage Legumes, F Paolocci, G Tanner, S Arcioni, F Damiani
IGC Proceedings (1997-2023)
The transformation of Lotus corniculatus plants with the maize gene Sn, reorganizes the tissue specificity of condensed tannins accumulation. In particular the transformed plants show an increase of tannin content in roots and a decrease in leaves. Molecular and enzymatic analyses suggest that the transgene can functionally substitute an endogenous unknown gene not expressed in roots and induces its silencing when it is expressed. These findings could have applications for reducing tannin content in unpalatable plants and for cloning genes involved in tannin synthesis.
Nutritional Implications Of Bound Proanthocyanidins, H P.S Makkar, K Becker
Nutritional Implications Of Bound Proanthocyanidins, H P.S Makkar, K Becker
IGC Proceedings (1997-2023)
The correlation between protein binding capacity of proanthocyanidin-rich neutral detergent fiber (NDF) and their proanthocyanidin (PAs) content was very low (r = -0.21; n = 6). These PAs-rich NDF did not affect growth of a tannin-sensitive strain of Clostridium perfringens. A substantial amount of bound PAs (75 to 92 %) disappeared from the NDF in 24 h of fermentation in an in vitro incubation medium containing rumen microbes. Addition of a tannin-binding agent, polyethylene glycol to the incubation medium containing rumen microbes and browses made free of extractable tannins or NDF rich in bound PAs increased the gas production up …
Genetic Manipulation Of Sainfoin Leaf Tannins, M Y. Gruber, P Auser, G Lees, A D. Muir, M Wood
Genetic Manipulation Of Sainfoin Leaf Tannins, M Y. Gruber, P Auser, G Lees, A D. Muir, M Wood
IGC Proceedings (1997-2023)
An isogenic population of sainfoin (Onobrychis viciifolia Scop.) plants was developed by two methods; regeneration after long term tissue culture and plant transformation with an antisense chalcone synthase gene. Tannin (proanthocyanidin) content could be dramatically increased in untransformed callus by long term culture, but was not significantly altered in regenerating plantlets. Some of the mature transgenic plants had dramatically reduced levels of leaf tannin. The antisense gene was eliminated from all transgenic plants later in development.
Induction Of Mutants With Ectopic Expression Of Condensed Tannins, M Y. Gruber, B Skadhauge, P Auser, A D. Muir, K K. Thomsen, J Stougaard, B Coulman, D Von Wettstein
Induction Of Mutants With Ectopic Expression Of Condensed Tannins, M Y. Gruber, B Skadhauge, P Auser, A D. Muir, K K. Thomsen, J Stougaard, B Coulman, D Von Wettstein
IGC Proceedings (1997-2023)
Leaves of 47,000 Lotus japonicus plants were screened using a butanol:HCl histochemical test to select “gain of function” mutants. These plants were progeny from L. japonicus lines which were transformed with T-DNA constructs containing either the maize Ds or Ac transposon (Thykjaer et al., 1995). Among 21 putative leaf tannin mutants, five (tan1-5) were characterized for synthesis of condensed tannins, leucocyanidin reductase activity and the presence of Ac and the selectable marker gene, nptII . A range of leaf tannin content among other Lotus species was also characterized.
The Effect Of Condensed Tannins In Lotus Spp. On The Lesser Migratory Grasshopper, C F. Hinks, G L. Lees, M Y. Gruber, A D. Muir, J Soroka
The Effect Of Condensed Tannins In Lotus Spp. On The Lesser Migratory Grasshopper, C F. Hinks, G L. Lees, M Y. Gruber, A D. Muir, J Soroka
IGC Proceedings (1997-2023)
Hatchling grasshoppers (Melanoplus sanguinipes Fab.) fed one accession of field grown big trefoil (Lotus uliginosis Schkuhr) containing a tannin content of 56 mg. g-1 FW, had a significantly lower mean weight than hatchlings fed field grown Lotus species and accessions with lower tannin content. Adult female grasshoppers fed L. uliginosis foliage with 32.3 mg.g-1 FW tannin also ate more feed and had a 2-fold higher gut trypsin and chymotrypsin activity than adults fed low tannin L. corniculatus foliage (0.13 mg.g-1 FW tannin). In more controlled experiments, growth at 20o C or 30o C was used to increase tannin …
Genotypic Effects On Condensed Tannins In The Leucaena Genus, S A. Dalzell, H M. Shelton
Genotypic Effects On Condensed Tannins In The Leucaena Genus, S A. Dalzell, H M. Shelton
IGC Proceedings (1997-2023)
One hundred and eighteen accessions of the Leucaena genus were assayed for extractable and bound condensed tannins (CT). Leucaena taxa varied from low or no CT (0-1%) to extremely high levels (>15%). There was considerable intraspecific variation in CT within key taxa. The proportion of bound tannin decreased with increasing total CT content.
Effects Of Incorporation Of Sainfoin (Onobrychis Viciafolia Scop.) With Cool Season Grasses On In Vitro Digestibility And Ch4 Emission, H. P. Poudel, Y. Wang, T. Mcallister, Z. J. Zu, B. Eisenreich, S. Acharya
Effects Of Incorporation Of Sainfoin (Onobrychis Viciafolia Scop.) With Cool Season Grasses On In Vitro Digestibility And Ch4 Emission, H. P. Poudel, Y. Wang, T. Mcallister, Z. J. Zu, B. Eisenreich, S. Acharya
IGC Proceedings (1997-2023)
Sainfoin (Onobrychis viciafolia Scop.) is an important non-bloating perennial leguminous forage. The tannins in sainfoin alter protein metabolism in the rumen and have been implicated in altering both nitrous oxide and methane emissions. However, the effect of sainfoin when mixed with cool-season forages is unknown. Therefore, in this study, we evaluated the in-vitro fermentation of sainfoin hay mixed with two other perennial cool-season forages, meadow bromegrass and orchardgrass at five ratios (0:100, 25:75, 50:50, 75:50, and 100:0). Data on dry matter disappearance (DMD), neutral detergent fiber disappearance (NDFD), gas production (GP) methane (CH4) emissions, and ammonia production were collected …
Tannins In Perennial Legume And Forb Functional Forages, J. Macadam, Juan Villalba, S. Lagrange, E. Stewart, S. Hunt, J. Legako, R. Christensen, R. L. Pitcher, A. Bolletta
Tannins In Perennial Legume And Forb Functional Forages, J. Macadam, Juan Villalba, S. Lagrange, E. Stewart, S. Hunt, J. Legako, R. Christensen, R. L. Pitcher, A. Bolletta
IGC Proceedings (1997-2023)
Feed is the greatest input cost for cattle producers. The studies summarized here employed non-bloating, tannin-containing irrigated perennial legume pastures or hay of legumes or a hydrolysable tannin-containing forb that were grown in the Mountain West USA, with non-tannin legume, grass, or feedlot treatments for comparison. Cattle grazing legume pastures or fed legume or forb hays had greater intake, gain and nitrogen retention, and in some cases, reduced enteric methane emissions compared with grass pastures or hay, and methane emissions were not different from feedlot-fed cattle.
Concentration And Bioactivity Of Condensed Tannins And Total Phenolics Of Lespedeza Species From A Germplasm Collection, Thomas H. Terrill, V. Smith, B. Morning, E. M. Courson, James P. Muir, N. M. Cherry, J. B. Morris
Concentration And Bioactivity Of Condensed Tannins And Total Phenolics Of Lespedeza Species From A Germplasm Collection, Thomas H. Terrill, V. Smith, B. Morning, E. M. Courson, James P. Muir, N. M. Cherry, J. B. Morris
IGC Proceedings (1997-2023)
Interest in ‘AU-Grazer’ sericea lespedeza (Lespedeza cuneata) as an anti-parasitic bioactive forage has been growing in the United States, but the concentration and bioactivity of tannins and other phenolics from additional accessions of L. cuneata, as well as other Lespedeza species, have not been evaluated. A study was completed to determine the concentration of extractable condensed tannins (ECT), total CT (TCT), total phenolics (TP), and protein-precipitable phenolics (PPP) of 32 accessions of L. cuneata and 16 additional Lespedeza species from a germplasm collection. The plants were established in small pots in a greenhouse and then transplanted into …
Proanthocyanidins From Hedysarum, Lotus And Onobrychis Spp. Growing In Sardinia And Sicily And Their Antioxidant Activity, A. Tava, M. G. De Benedetto, D. Tedesco, G. Di Miceli, G. Piluzza
Proanthocyanidins From Hedysarum, Lotus And Onobrychis Spp. Growing In Sardinia And Sicily And Their Antioxidant Activity, A. Tava, M. G. De Benedetto, D. Tedesco, G. Di Miceli, G. Piluzza
IGC Proceedings (1997-2023)
Proanthocyanidins (PA), or condensed tannins, are a class of natural polyphenolic compounds, occurring in numerous plant species, including a number of economically significant forage legumes. These compounds are polymers of flavan-3-ols, and typically contain from 2 to 20 units. Their biological significance is still being debated and, in recent years, a great deal of attention has been focused on their role in ruminant nutrition. Evidence has indicated that PA, in a moderate concentration (0.5-5% DM), may have considerable importance in protecting dietary proteins against microbial degradation in the rumen, and in preventing bloat. The antioxidant activity (AA) is also an …
Proanthocyanidins From Hedysarum, Lotus And Onobrychis Spp. Growing In Sardinia And Sicily And Their Antioxidant Activity, A. Tava, M. G. De Begedetto, D. Tedesco, G. Di Miceli, G. Piluzza
Proanthocyanidins From Hedysarum, Lotus And Onobrychis Spp. Growing In Sardinia And Sicily And Their Antioxidant Activity, A. Tava, M. G. De Begedetto, D. Tedesco, G. Di Miceli, G. Piluzza
IGC Proceedings (1997-2023)
Proanthocyanidins (PA), or condensed tannins, are a class of natural polyphenolic compounds, occurring in numerous plant species, including a number of economically significant forage legumes. These compounds are polymers of flavan-3-ols, and typically contain from 2 to 20 units. Their biological significance is still being debated and, in recent years, a great deal of attention has been focused on their role in ruminant nutrition. Evidence has indicated that PA, in a moderate concentration (0.5-5% DM), may have considerable importance in protecting dietary proteins against microbial degradation in the rumen, and in preventing bloat. The antioxidant activity (AA) is also an …
Use Of Green Sulla Forage For Feeding. 2. Effects On Lamb Carcass And Meat Quality, A. Bonanno, M. L. Alicata, G. Tornambè, F. Mazza, G. Di Miceli, D. Giambalvo
Use Of Green Sulla Forage For Feeding. 2. Effects On Lamb Carcass And Meat Quality, A. Bonanno, M. L. Alicata, G. Tornambè, F. Mazza, G. Di Miceli, D. Giambalvo
IGC Proceedings (1997-2023)
Diets with 2.5% of condensed tannins (CT) from carob pulp have been showed to reduce lamb carcass weight, yield and fatness, as a consequence of lower digestibility due to strong protein-tannin bonds. Moreover the diets lightened meat colour and negatively affected meat sensory properties (Priolo et al., 2000). There is evidence that some tannin-rich legumes have weaker stability of the protein-tannin complex post-rumen (McSweeney et al., 2001). Since the CT-containing sulla (Hedysarum coronarium L.) demonstrated improved lamb growth performance in comparison with CT-lacking annual ryegrass (Lolium multiflorum Lam. subsp. wersterwoldicum) (Giambalvo et al., 2005), …
Use Of Green Sulla Forage For Feeding. 1. Effects On Lamb Growth And Gastrointestinal Nematode Parasite Infestation, D. Giambalvo, A. Di Grigoli, M. L. Alicata, B. Formoso, A. S. Frenda, P. Trapani
Use Of Green Sulla Forage For Feeding. 1. Effects On Lamb Growth And Gastrointestinal Nematode Parasite Infestation, D. Giambalvo, A. Di Grigoli, M. L. Alicata, B. Formoso, A. S. Frenda, P. Trapani
IGC Proceedings (1997-2023)
Recent studies have shown that some forage legumes containing condensed tannins (CT), such as sulla (Hedysarum coronarium L.), can reduce the gastrointestinal nematode burden in sheep (Niezen et al., 1998) and increase post-ruminal protein availability (Waghorn et al., 1994). This study aimed to evaluate the anthelmintic and nutritional properties of sulla forage in relation to its CT content. Thus, the growth performance and the level of nematode infestation of lambs fed sulla were compared with those of lambs fed ryegrass (Lolium multiflorum Lam. subsp. wersterwoldicum), lacking in CT.
Condensed Tannins In Different Varieties Of Lotus Corniculatus, Christina L. Marley, Rhun Fychan, R. Jones
Condensed Tannins In Different Varieties Of Lotus Corniculatus, Christina L. Marley, Rhun Fychan, R. Jones
IGC Proceedings (1997-2023)
Research has shown that Lotus corniculatus may provide benefits as a grazing forage for ruminants, because of improved efficiency of protein utilisation in part due to the presence of condensed tannins (CT) in the forage. Lotus species have been found to contain different CT (Aerts et al., 1999). The aim of this experiment was to determine if differences exist in CT concentration among L. corniculatus varieties.
Light Intensity Is Positively Correlated With The Synthesis Of Condensed Tannins In Lotus Corniculatus, Sergio Arcioni, T. Bovone, F. Damiani, F. Paolocci
Light Intensity Is Positively Correlated With The Synthesis Of Condensed Tannins In Lotus Corniculatus, Sergio Arcioni, T. Bovone, F. Damiani, F. Paolocci
IGC Proceedings (1997-2023)
The importance of Condensed Tannins (CT) in forage legumes has been well documented in several studies. The role of plant genetics in this field is the acquisition of competences in order to be able to modulate CT synthesis in leaves of these species. The role of light has been investigated in this work on the increase of condensed tannin levels in leaves of two contrasting genotypes of birdsfoot trefoil (Lotus corniculatus).
Condensed Tannin Concentration And Herbage Accumulation Of A White Clover Bred For Increased Floral Condensed Tannin, V. T. Burggraaf, S. L. Woodward, D. R. Woodfield, E. R. Thom, G. C. Waghorn, P. D. Kemp
Condensed Tannin Concentration And Herbage Accumulation Of A White Clover Bred For Increased Floral Condensed Tannin, V. T. Burggraaf, S. L. Woodward, D. R. Woodfield, E. R. Thom, G. C. Waghorn, P. D. Kemp
IGC Proceedings (1997-2023)
White clover is a high quality feed for ruminants, however, its high protein content results in excessive urea excretion in urine and can cause bloat, reducing its potential value for animal production. The condensed tannins (CT) in some forages can reduce these problems, but plants may have poor agronomic performance. White clover produces CT in its flower heads, but herbage CT concentrations are normally too low to benefit animals. This paper reports CT concentrations and herbage accumulation over 2 years of an experimental line of white clover (HT) selected for increased flowering and floral CT concentrations.
High Floral Tannin White Clover Reduces Rumen Ammonia Concentrations In Dairy Cows, V. T. Burggraaf, G. C. Waghorn, S. L. Woodward, D. R. Woodfield, E. R. Thom, P. D. Kemp
High Floral Tannin White Clover Reduces Rumen Ammonia Concentrations In Dairy Cows, V. T. Burggraaf, G. C. Waghorn, S. L. Woodward, D. R. Woodfield, E. R. Thom, P. D. Kemp
IGC Proceedings (1997-2023)
White clover produces high quality forage for ruminant production, but it results in high rumen ammonia concentrations, indicating extensive protein degradation. The excess ammonia is absorbed through the rumen and excreted as urea in urine, at a cost to the animal and the environment. Condensed tannins (CT) contained in some forages reduce proteolysis in the rumen, which can lead to increased amino acid absorption and therefore improved animal performance. White clover produces CT in its flower heads, but concentrations are normally too low to benefit animals. This paper reports on comparisons of rumen ammonia concentrations in dairy cows grazing HT …
Effect Of Three Legumes Containing Different Condensed Tannin Concentrations On The In Vitro Formation Of The Pastoral Flavour Compound; Skatole, N. M. Schreurs, M. H. Tavendale, G. A. Lane, T. N. Barry, W. C. Mcnabb
Effect Of Three Legumes Containing Different Condensed Tannin Concentrations On The In Vitro Formation Of The Pastoral Flavour Compound; Skatole, N. M. Schreurs, M. H. Tavendale, G. A. Lane, T. N. Barry, W. C. Mcnabb
IGC Proceedings (1997-2023)
Feeding legumes, such as white clover (Trifolium repens), results in higher intakes and increased animal production compared to grasses (Ulyatt, 1981). Skatole is produced in the rumen from plant protein fermentation and is associated with undesirable pastoral flavours in meat (Young et al. 2002). Feeding white clover causes a greater skatole concentration in the rumen compared to perennial ryegrass (Lolium perenne) or Lotus corniculatus, as the protein in white clover is highly soluble and rapidly degraded (Schreurs et al., 2004). The condensed tannins (CT) in Lotus species slow protein degradation in the rumen (Aerts …
Minimising Bloat Through Development Of White Clover (T. Repens) With High Levels Of Condensed Tannins, M. T. O’Donoghue, C. Spillane, E. Guiney
Minimising Bloat Through Development Of White Clover (T. Repens) With High Levels Of Condensed Tannins, M. T. O’Donoghue, C. Spillane, E. Guiney
IGC Proceedings (1997-2023)
White clover constitutes a low percentage of the overall sward content in Irish pastureland despite EU directives limiting the use of nitrogenous fertilizers. This is mainly due to the tendency of large amounts of white clover to cause bloat. Bloat is a potentially fatal build up of proteinaceous foam in the guts of ruminants. Some lesser cultivated legumes such as Lotus species contain condensed tannins (CT) that decrease the incidence of bloated animals. The project’s objective is to reduce the risk of bloat by generating white clover cultivars with high CT content. We are investigating whether expression of the ANTHOCYANIN …
Polyphenolic Phenomena: Transgenic Analysis Of Some Of The Factors That Regulate The Cell-Specific Accumulation Of Condensed Tannins (Proanthocyanidins) In Forage Crops, Mark P. Robbins, G. Allison, D. Bryant, P. Morris
Polyphenolic Phenomena: Transgenic Analysis Of Some Of The Factors That Regulate The Cell-Specific Accumulation Of Condensed Tannins (Proanthocyanidins) In Forage Crops, Mark P. Robbins, G. Allison, D. Bryant, P. Morris
IGC Proceedings (1997-2023)
Condensed tannins biosynthesised within crops are a well-established mechanism for protecting plant protein in the rumen of grazing livestock. Protein protection mediated by these polymeric flavonoid molecules has been characterised in Lotus spp. and offers an interesting contrast to the polyphenol oxidase (PPO) system that confers protein protection in red clover.
Foliar Expression Of Candidate Genes Involved In Condensed Tannin Biosynthesis In White Clover (Trifolium Repens), Stephen N. Panter, J. Simmonds, A. Winkworth, A. Mouradov, G. C. Spangenberg
Foliar Expression Of Candidate Genes Involved In Condensed Tannin Biosynthesis In White Clover (Trifolium Repens), Stephen N. Panter, J. Simmonds, A. Winkworth, A. Mouradov, G. C. Spangenberg
IGC Proceedings (1997-2023)
Bloat disease in cattle and sheep is caused by the rapid microbial degradation of protein-rich fodder in the rumen. This leads to the production of protein foams that trap gases, causing bloat, a condition that is often fatal to livestock and costly to farmers. Condensed tannins (CTs) are phenolic polymers produced by the phenylpropanoid pathway of plants (Figure 1). CTs bind to proteins under acidic to neutral conditions, such as those present in the rumen, slowing their breakdown. A diet with a CT content of between 2% and 4% by dry weight, which is provided by some pasture legumes (e.g. …
Role Of The Banyuls(Ban) Gene From Arabidopsis Thaliana In Transgenic Alfalfa Expression Of Anthocyanins And Proanthocyanidins, S. M. Hesamzadeh Hejazi, Sergio Arcioni, F. Paolocci
Role Of The Banyuls(Ban) Gene From Arabidopsis Thaliana In Transgenic Alfalfa Expression Of Anthocyanins And Proanthocyanidins, S. M. Hesamzadeh Hejazi, Sergio Arcioni, F. Paolocci
IGC Proceedings (1997-2023)
Condensed tannins (CTs) are flavonoid oligomers, many of which have beneficial effects on animal (bloat safe) and human health. The BAN gene encodes anthocyanidin reductase (ANR), an enzyme proposed to convert anthocyanidins to their corresponding 2,3-cis-flavan-3-ols (Xie et al., 2003). Ectopic expression of BAN in Alfalfa transgenic foliage results in accumulation of CTs. Thus, it has been assumed that the BAN gene also acts in starter units for the condensation of tannins in Alfalfa.
Effects Of Feeding Legume Silage With Differing Tannin Levels On Lactating Dairy Cattle, U. C. Hymes Fecht, G. A. Broderick, R. E. Muck
Effects Of Feeding Legume Silage With Differing Tannin Levels On Lactating Dairy Cattle, U. C. Hymes Fecht, G. A. Broderick, R. E. Muck
IGC Proceedings (1997-2023)
Condensed tannins (CT) bind to plant proteins in the rumen, reducing protein degradation to ammonia and increasing milk production and milk protein (e.g. Waghorn, 1987). Previous research showed that the reduced soluble non-protein nitrogen (NPN) content of red clover (Trifolium pratense) silage (RCS) was related to its greater N efficiency relative to lucerne (Medicago sativa) silage (LS) (Broderick et al., 2001). Commercial cultivars of birdsfoot trefoil (Lotus corniculatus; BFT) contain modest levels of CT which reduce NPN formation in silage (Albrecht & Muck, 1991). The objective was to compare silages made from BFT …