Physical Sciences and Mathematics Commons^™

Tryptophan-Like Fluorescence And Non-Point Source Pollution In Karst Basins, Inner Bluegrass Region, Kentucky, Ryan Dapkus

Theses and Dissertations--Earth and Environmental Sciences

Typically, the degree of fecal contamination of water is assessed by the concentration of thermotolerant coliforms, like E. coli. This method is time-consuming, taking > 18 hours between the start of incubation and subsequent enumeration. The current study examines the utility of monitoring tryptophan-like fluorescence (TLF) using in-situ fluorometers as a proxy for fecal contamination, particularly E. coli. Relative timing of discharge peaks and TLF peaks is considered, as well as differences in E. coli concentrations and major anion concentrations between two different basins that were monitored weekly. Results indicate that TLF is somewhat useful in the prediction of …

Metal-Resistance Genetically Engineered Bacteria, Sylvia Daunert, Donna Scott, Sridhar Ramanathan

KWRRI Research Reports

Bacterial-based electrochemical and optical sensing systems that respond in a highly selective and sensitive manner to antimonite and arsenite have been developed. This was accomplished by using genetically engineered bacteria bearing one of two plasmids constructed for our studies. The first plasmid, pBGD23, contains the operator/promoter region (O/P) and the gene of the ArsR protein from the ars operon upstream from the β-galactosidase gene. In the absence of antimonite/arsenite, ArsR binds to the 0/P site and prevents the transcription of the genes for ArsR and β-galactosidase, thus blocking expression of these proteins. When antimonite or arsenite is present in the …

Use Of Riparian Vegetated Filter Strips To Reduce Nitrate And Fecal Contamination In Surface Water, Mark S. Coyne, Robert L. Blevins, Rebecca A. Gilfillen

KWRRI Research Reports

This research assessed fecal bacteria trapping in surface runoff by grass filters and their potential to enhance NO₃^- removal via denitrification. Grass filter strips 9.0 m long trapped over 99% of the soil in surface runoff in 1992. Fecal coliform removal was less than 75%. In 1993, 9.0 and 4.5 m grass filter strips trapped 99 and 95% of the sediment, respectively. Fecal coliform trapping efficiency was 90% in 9.0 m grass filters and 75% in 4.5 m filters. Fecal streptococci trapping efficiency was 77% in 9.0 m grass filters and only 56% in 4.5 m filters. Fecal …

Identification Of Soil-Water Chemical Parameters For The Prediction And Treatment Of Suspended Solids In Surface Water Reservoirs Of Coal Mine Lands, V. P. Evangelou, J. H. Grove, R. I. Barnhisel

KWRRI Research Reports

High concentrations of suspended solids in coal mine sedimentation ponds are a factor in lowering water quality. Colloidal particle settling simulations were carried out in the laboratory to test the influence pH and dissolved solids have on concentration and settling rates of suspended solids. The results of the study reveal that the pH range of colloidal coflocculation for the samples tested is between 3.5 and 4.5. Furthermore, liming simulation of acidic sediments, as expected increased colloid dispersion. This increase was dependent on the magnitude of the sodium adsorption ratio (SAR). The greater SAR systems maintained a greater concentration of colloidal …

Identification Of Soil-Water Chemical Parameters For The Prediction And Treatment Of Suspended Solids In Surface Water Reservoirs Of Coal Mine Lands, V. P. Evangelou, J. H. Grove, R. I. Barnhisel

KWRRI Research Reports

High concentrations of suspended solids in coal mine sedimentation ponds are a factor in lowering water quality. This study focuses on the influence dissolved solids have on concentration and settling of suspended solids. Water samples from sedimentation ponds in Eastern and Western Kentucky were used to evaluate water composition in such ponds. Spoil samples from surface mine sites in both parts of the state were used to evaluate water composition released from the spoils upon introducing water.

The results demonstrate that water quality emanating from coal spoils of Eastern and Western coal mines is dependent on the type of spoil …

The Aquatic Toxicity Of Organic Compounds To Embryo-Larval Stages Of Fish And Amphibians, Jeffrey A. Black, Wesley J. Birge, Willaim E. Mcdonnell, Albert G. Westerman, Barbara A. Ramey, Donald M. Bruser

KWRRI Research Reports

Aquatic toxicity tests were conducted on 11 organic compounds considered hazardous to water resources. The toxicity of each compound was evaluated using embryo-larval stages of two to eight fish and amphibian species. Exposure was initiated at fertilization and maintained through 4 days posthatching. The animal test species exhibited varying degrees of sensitivity to the selected toxicants. Combined frequencies for mortality and teratogenesis at 4 days posthatching gave LC₅₀ ranges of 3.66 to 8.25 mg/L for benzene, 1.16 to 22.42 mg/L for carbon tetrachloride, 0.11 to 1.20 mg/L for chlorobenzene, 2.03 to > 68 mg/L for chloroform, 3.01 to 5.56 mg/L …

An Environmental Study Of The Origin, Distribution, And Bioaccumulation Of Selenium In Kentucky And Barkley Lakes, B. E. Mcclellan, Kenneth J. Frazer

KWRRI Research Reports

Many samples of water, bottom sediment, and fish were analyzed for toxic metal ion content. The samples were collected from several selected sites along Kentucky and Barkley Lakes as well as the Cumberland River and several sub-impoundments along these aquatic systems. Emphasis was placed on selenium, although several other metal ions were determined. The results showed that there are no serious pollution problems with As, Cd, Cr, Cu, Hg, Pb, Se, Sr, Zn, or Zr at any of the sites examined. Actually, none of the trace metals examined even come close to the EPA limits on fish, with the exception …

Emission Of Microbial Aerosols From Polluted Waters In Densely Populated Regions, Lois S. Cronholm

KWRRI Research Reports

The air surrounding three activated sludge tanks was sampled over a two year period for the emission of bacterial aerosols under a variety of climatic conditions and at varying distances upwind and downwind of the aerated tanks. All plants emitted species of enteric bacteria which are significant as index organisms and as frank pathogens. The emission pattern of these bacteria were influenced by distance from the plant and wind direction. Within the parameters of a plant, defined arbitrarily in this study by sampling sites less than 150 m upwind and less than 900 m downwind, distance from the source was …

Assessment Of The Water Quality In The Salt River Prior To Its Impoundment In Anderson And Spencer Counties, Kentucky, Andrew C. Miller, Louis A. Krumholz, Stuart E. Neff, Darly E. Jennings, Patricia B. Olmes, David S. White

KWRRI Research Reports

Monthly water samples were taken and analyzed to determine the water quality of the Salt River in Anderson and Spencer counties Kentucky prior to the river's impoundment. Sediments from the area watershed were analyzed to total acid digestion, barium chloride extraction, and aqueous extraction methods. Rainwater and runoff water were collected and analyzed for major cations and anions from two sites in the watershed.

The Salt River at Taylorsville is characterized by hard water with high levels of calcium (33.5-74.8 mg/1), bicarbonate (136-236 mg/l), specific conductance (200-535 μmhos/cm), and sulfate (16.5-71.5 mg/l). Nitrates (0.6-5.7 mg/l), phosphates (0.2-2.4 mg/l), sodium (3.2-20.3 …

Enteric Virus Survival In Package Plants And The Upgrading Of The Small Treatment Plants Using Ozone, Lois S. Cronholm, James R. Mccammon, Marvin Fleischman, Jerry R. Perrich, Valerie Reisser, William Harris, Ronald R. Vanstockum, Khosrow Jaberizadeh, Michael J. Wahl

KWRRI Research Reports

Post-chlorinated effluent collected with a portable viral concentrator from four treatment plants in Jefferson County, Kentucky, yielded infective viral particles from three plants from spring through late fall. The pH, ,chlorine, turbidity, and coliform levels of these effluents indicated that viral persistence was correlated with inefficient processing which produced effluent environments that inhibited disinfection by chlorine. The disinfection potential of ozone was tested on secondary effluent and finished water seeded with poliovirus and Esaheriahia coli. Low doses of ozone inactivated viruses and bacteria in treated water, but not in effluent. The inactivation of bacteria by ozone does not appear …

Seasonal Changes In Water Quality And Primary Productivity In Doe Valley Lake, Edmond J. Bacon, Stuart E. Neff

KWRRI Research Reports

Primary productivity and water quality were studied in Doe Valley Lake, a 147-hectare impoundment on Doe Run, a spring-fed stream in Meade County, Kentucky, from 13 June 1969 to 31 July 1972. Doe Valley Lake is monomictic during most winter seasons, but it is dimictic during more severe winters because of its morphometry and location on the borderline climatic region for dimictic lakes (37° N latitude). Oxygen depletion in the hypolimnion is severe, and anaerobic conditions usually prevail by late June. A hypolimnetic areal deficit of 0.038 mg/cm²/day was calculated. Supersaturation of oxygen in the epilimnion was common, …

Enzyme And Tissue Alterations In Fishes: A Measure Of Water Quality, David E. Hinton, M. W. Kendall, J. C. Koenig

KWRRI Research Reports

A variety of freshwater fishes were studied by light and electron microscopy, enzyme histochemical and biochemical methods, The objective was to determine normal structure and function in specific target organs and to compare these to altered states in aquatic pollution. The basic question, "can fish tissues and enzymes serve as indicators of water quality?," was asked. Microscopic alteration in gill was indicative of copper toxicity at an exposure of 20 parts per billion, Gross and light microscopic alterations were indicative of a single exposure of channel catfish to 15 parts per million of methyl mercuric chloride (CH₃HgCl). Microscopic …

A Preliminary Ecological Study Of Areas To Be Impounded In The Salt River Basin Of Kentucky, Louis A. Krumholz, Stuart E. Neff, Edmond J. Bacon, Jerry S. Parsons, John D. Woodling

KWRRI Research Reports

This report includes work that is an extension of Project No. B-005-KY as reported in Research Report No. 43 of the University of Kentucky Water Resources Institute. That project was initiated in April 1968 as Project No. A-019-KY with principal emphasis on physical, chemical, and biological characteristics of the main stem of the Salt River upstream from the proposed damsite for Taylorsville Lake, an impoundment of about 3, 600 acres at seasonal pool. The report includes descriptions of an additional 13 stations along the stream, bringing to 38 the number of permanent collecting sites.

Values for dissolved oxygen ranged from …

A Preliminary Ecological Study Of Areas To Be Impounded In The Salt River Basin Of Kentucky, Louis A. Krumholz

KWRRI Research Reports

This report covers work that is an extension of Project No. A-019-KY. A series of 25 sampling stations was established in the mainstream and tributaries of the Salt River that extend from the source of the stream in Boyle County to a few miles below the site of Taylorsville Darn in Spencer County. Sampling for water chemistry and biota was carried out semimonthly. Data on temperature, oxygen, depth, and discharge, along with analyses for cations (Ca, Mg, Fe, Mn) and anions (PO₄, NO₃, NO₂, CO₃, HCO₃) have been accumulated and …