Physical Sciences and Mathematics Commons^™

Preferential Utilization Of Nadph As The Endogenous Electron Donor For Nad(P)H:Quinone Oxidoreductase 1 (Nqo1) In Intact Pulmonary Arterial Endothelial Cells, Robert D. Bongard, Brian J. Lindemer, Gary S. Krenz, Marilyn P. Merker

Mathematics, Statistics and Computer Science Faculty Research and Publications

The goal was to determine whether endogenous cytosolic NAD(P)H:quinone oxidoreductase 1 (NQO1) preferentially uses NADPH or NADH in intact pulmonary arterial endothelial cells in culture. The approach was to manipulate the redox status of the NADH/NAD⁺ and NADPH/NADP⁺ redox pairs in the cytosolic compartment using treatment conditions targeting glycolysis and the pentose phosphate pathway alone or with lactate, and to evaluate the impact on the intact cell NQO1 activity. Cells were treated with 2-deoxyglucose, iodoacetate, or epiandrosterone in the absence or presence of lactate, NQO1 activity was measured in intact cells using duroquinone as the electron acceptor, and …