Open Access. Powered by Scholars. Published by Universities.®
Physical Sciences and Mathematics Commons™
Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 4 of 4
Full-Text Articles in Physical Sciences and Mathematics
Synthesis And Hydrolytic Evaluation Of Acid-Labile Imine-Linked Cytoxic Isatin Model Systems, Lidia Matesic, Julie Locke, Kara Perrow, Marie Ranson, John Bremner, Danielle Skropeta
Synthesis And Hydrolytic Evaluation Of Acid-Labile Imine-Linked Cytoxic Isatin Model Systems, Lidia Matesic, Julie Locke, Kara Perrow, Marie Ranson, John Bremner, Danielle Skropeta
Danielle Skropeta
In this study a series of isatin-based, pH-sensitive aryl imine derivatives with differing aromatic substituents and substitution patterns were synthesised and their acid-catalysed hydrolysis evaluated. These derivatives were functionalised at the C3 carbonyl group of a potent N-substituted isatin cytotoxin and were stable at physiological pH but readily cleaved at pH 4.5. Observed rates of hydrolysis for the embedded imine-acid moiety were in the order para-phenylpropionic acid>phenylacetic acid (para>meta)>benzoic acid (meta>para). The ability to fine-tune hydrolysis rates in this way has potential implications for optimising imine linked, tumour targeting cytotoxin-protein conjugates
Assessment Of Length Distributions Between Non-Coding And Coding Sequences Amongst Two Model Organisms, Ren Zhang, Rachel Caldwell, Yan-Xia Lin
Assessment Of Length Distributions Between Non-Coding And Coding Sequences Amongst Two Model Organisms, Ren Zhang, Rachel Caldwell, Yan-Xia Lin
Associate Professor Yan-Xia Lin
No abstract provided.
Flexible Free-Standing Carbon Nanotube Films For Model Lithium-Ion Batteries, Jun Chen, Sau Yen Chew, See How Ng, Jiazhao Wang, Hua Liu, P Novak, F Krumeich, Shulei Chou
Flexible Free-Standing Carbon Nanotube Films For Model Lithium-Ion Batteries, Jun Chen, Sau Yen Chew, See How Ng, Jiazhao Wang, Hua Liu, P Novak, F Krumeich, Shulei Chou
Shulei Chou
No abstract provided.
Chemical Cross-Linking Of The Chloroplast Localized Small Heat-Shock Protein, Hsp21, And The Model Substrate Citrate Synthase, Emma Ahrman, W Lambert, Andrew Aquilina, C V Robinson, Cs Emanuelsson
Chemical Cross-Linking Of The Chloroplast Localized Small Heat-Shock Protein, Hsp21, And The Model Substrate Citrate Synthase, Emma Ahrman, W Lambert, Andrew Aquilina, C V Robinson, Cs Emanuelsson
J. A. Aquilina
The molecular mechanism whereby the small heat-shock protein (sHsp) chaperones interact with and prevent aggregation of other proteins is not fully understood. We have characterized the sHsp-substrate protein interaction at normal and increased temperatures utilizing a model substrate protein, citrate synthase (CS), widely used in chaperone assays, and a dodecameric plant sHsp, Hsp21, by chemical cross-linking with 3,3'-Dithiobis[sulfosuccinimidylpropionate] (DTSSP) and mass spectrometric peptide mapping. In the absence of CS, the cross-linker captured Hsp21 in dodecameric form, even at increased temperature (47 degrees C). In the presence of equimolar amounts of CS, no Hsp21 dodecamer was captured, indicating a substrate-induced Hsp21 …