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Full-Text Articles in Physical Sciences and Mathematics
Competitive Evaluation Of Data Mining Algorithms For Use In Cassification Of Leukocyte Subtypes With Raman Microspectroscopy, Adrian Maguire, I. Vega-Carrascal, Jane Bryant, Lisa White, Orla Howe, Fiona Lyng, Aidan Meade
Competitive Evaluation Of Data Mining Algorithms For Use In Cassification Of Leukocyte Subtypes With Raman Microspectroscopy, Adrian Maguire, I. Vega-Carrascal, Jane Bryant, Lisa White, Orla Howe, Fiona Lyng, Aidan Meade
Articles
Raman microspectroscopy has been investigated for some time for use in label-free cell sorting devices. These approaches require coupling of the Raman spectrometer to complex data mining algorithms for identification of cellular subtypes such as the leukocyte subpopulations of lymphocytes and monocytes. In this study, three distinct multivariate classification approaches, (PCA-LDA, SVMs and Random Forests) are developed and tested on their ability to classify the cellular subtype in extracted peripheral blood mononuclear cells (T-cell lymphocytes from myeloid cells), and are evaluated in terms of their respective classification performance. A strategy for optimisation of each of the classification algorithm is presented …
Extending And Merging The Purple Crow Lidar Temperature Rayleigh And Vibrational Raman Climatologies, Ali Jalali
Extending And Merging The Purple Crow Lidar Temperature Rayleigh And Vibrational Raman Climatologies, Ali Jalali
Electronic Thesis and Dissertation Repository
Rayleigh and Raman scatter measurements from The University ofWestern Ontario Purple Crow Lidar (PCL) have been used to develop temperature climatologies for the stratosphere, mesosphere, and thermosphere using data from 1994 to 2013 (Rayleigh system) and from 1999 to 2013 (vibrational Raman system). Temperature retrievals from Rayleigh-scattering lidar measurements have been performed using the methods by Hauchecorne and Chanin (1980; henceforth HC) and Khanna et al. (2012). Argall and Sica (2007) used the HC method to compute a climatology of the PCL measurements from 1994 to 2004 for 35 to 110 km, while Iserhienrhien et al. (2013) applied the same …
Effect Of Substrate Choice And Tissue Type On Tissue Preparation For Spectral Histopathology By Raman Microspectroscopy, Hugh Byrne, Leanne M. Fullwood, Dave Griffiths, Katherine Ashton, Timothy Dawson, Robert W. Lea, Charles Davis, Franck Bonnier, Matthew J. Baker
Effect Of Substrate Choice And Tissue Type On Tissue Preparation For Spectral Histopathology By Raman Microspectroscopy, Hugh Byrne, Leanne M. Fullwood, Dave Griffiths, Katherine Ashton, Timothy Dawson, Robert W. Lea, Charles Davis, Franck Bonnier, Matthew J. Baker
Articles
Raman spectroscopy is a non-destructive, non-invasive, rapid and economical technique which has the potential to be an excellent method for the diagnosis of cancer and understanding disease progression through retrospective studies of archived tissue samples. Historically, biobanks are generally comprised of formalin fixed paraffin preserved tissue and as a result these specimens are often used in spectroscopic research. Tissue in this state has to be dewaxed prior to Raman analysis to reduce paraffin contributions in the spectra. However, although the procedures are derived from histopathological clinical practice, the efficacy of the dewaxing procedures that are currently employed is questionable. Ineffective …