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Full-Text Articles in Physical Sciences and Mathematics
A Study In Cross-Beam Polarization Fluorescence Photoactivation Localization Microscopy On Dendra2-Hemagluttinin In Fixed Nih3t3 Cells, Matthew M. Valles
A Study In Cross-Beam Polarization Fluorescence Photoactivation Localization Microscopy On Dendra2-Hemagluttinin In Fixed Nih3t3 Cells, Matthew M. Valles
Electronic Theses and Dissertations
Fluorescence microscopy is popular for its noninvasive properties and its use in imaging multiple species, simultaneously. Furthermore, superresolution fluorescence microscopy (SRFLM) utilizes photoswitchable proteins to improve the lateral resolution of conventional fluorescence microscopy by an order of magnitude. There is little work conducted on the study of excitation laser polarizations and their effect on the number of localizations as well as the brightness of molecules. This thesis attempts to study the effect of excitation wavelength polarization on the number of localizations and the brightness of molecules by comparing two orientations of circularly-polarized, excitation lasers. The first type of orientation involves …
A Novel Setup For High-Pressure Raman Spectroscopy Under A Microscope, Thomas Andrew Oakeson
A Novel Setup For High-Pressure Raman Spectroscopy Under A Microscope, Thomas Andrew Oakeson
Electronic Theses and Dissertations
Functional properties of biological molecules and cells are affected by environmental parameters such as temperature and pressure. While Raman spectroscopy provides an intrinsic probe of molecular structural changes, the incorporation of a microscope enables studies of minuscule amounts of biological compounds with spatial resolution on a micron scale. We have developed a novel setup which combines a Raman microscope and a high pressure cell. A micro-capillary made out of fused silica simultaneously serves as the supporting body and the optical window of the pressure cell. The cell has been tested over the pressure range from 0.1 to 4 kbar. Raman …