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Full-Text Articles in Physical Sciences and Mathematics
The Role Of Matp, Zapa And Zapb In Chromosomal Organization And Dynamics In Escherichia Coli, Jaana Männik, Daniel E. Castillo, Da Yang, George Siopsis, Jaan Männik
The Role Of Matp, Zapa And Zapb In Chromosomal Organization And Dynamics In Escherichia Coli, Jaana Männik, Daniel E. Castillo, Da Yang, George Siopsis, Jaan Männik
Physics and Astronomy Publications and Other Works
Despite extensive research over several decades, a comprehensive view of how the Escherichia coli chromosome is organized within the nucleoid, and how two daughter chromosomes segregate has yet to emerge. Here we investigate the role of the MatP, ZapA and ZapB proteins in organizing the replication terminus (Ter) region and in the chromosomal segregation process. Quantitative image analysis of the fluorescently labeled Ter region shows that the replication terminus attaches to the divisome in a single segment along the perimeter of the cell in a MatP, ZapA and ZapB-dependent manner. The attachment does not significantly affect the bulk chromosome segregation …