Physical Sciences and Mathematics Commons^™

Differential Effects Of Membrane Order On Membrane Permeability, Miranda J. Bradley, Marshall J. Colville, Miles J. Crumley, Drake C. Mitchell

Student Research Symposium

Phospholipid membranes segregate into lateral domains of liquid ordered (lo) and liquid disordered (ld) phases when cholesterol and mixed species of lipids with saturated and unsaturated acyl chains are present. To examine membrane permeability and rate of vesicle rupture by Triton in pure lo, pure ld, and mixed lo/ld phases, LUVs were prepared based on the ternary phase diagram of POPC, sphingomyelin, and cholesterol. These LUVs were loaded with 2mM carboxyfluorescein (CF) and formed by extrusion at 65oC. Using a stopped-flow fluorometer, changes in CF fluorescence were measured when LUVs were exposed to sudden osmotic gradients, pH gradients, or 0.1% …