Physical Sciences and Mathematics Commons^™

A Preliminary Characterization And Assessment Of Mesophotic Octocoral Microbiomes In The Western Gulf Of Mexico, Edward P. Gniffke

Theses and Dissertations

Mesophotic Coral Ecosystems are highly diverse and productive ecosystems in the western Gulf of Mexico which are composed of, in part, by octocorals (subclass Octocorallia). Despite their importance as foundational organisms octocorals are an understudied group in this region, with little known about their microbial community. Ninety-eight Octocoral samples collected from the western and northwestern Gulf of Mexico were sequenced using 16S rRNA sequencing to characterize their microbial communities. The sequenced microbiomes were generally low in diversity composed of a few core microbial taxa. Octocoral group was the main driver of microbiome composition as opposed to depth, season, region, and …

Effect Of Elevated Temperature And Roundup Exposure On Oxidative Stress And Antioxidant Status In The American Oyster, Afsana Chowdhury

Theses and Dissertations

Aquatic organisms are frequently exposed to various environmental stressors. In this study, we observed the short-term (1-week) combined dose-dependent effect of Roundup, a glyphosate-based herbicide, and high temperature (30°C) on the morphology of gills and digestive glands, expression of heat shock protein-70 (HSP70), dinitrophenyl protein (DNP), nitrotyrosine protein (NTP), antioxidant enzymes such as catalase (CAT) and superoxidase dismutase (SOD) in tissues of American oyster (Crassostrea virginica). Histological analyses showed an increase in mucous production in the gills and digestive glands, hemocyte aggregation in the connective tissues as well as structural change of the lumen in the digestive glands …

Flow Monitoring And Tidal Exchange Ratio Estimation For The Bahia Grande After Widening Of The Carl Joe Gayman Pilot Channel: Bahia Grande, Cameron County, Tx, Sabas Lopez Iii

Theses and Dissertations

The Bahia Grande (BG) is an 8,500-acre set of coastal bays and wetlands located between Brownsville and Port Isabel, Tx, near the Gulf of Mexico (GOM) coastline. In 2005, the Carl Joe Gayman Pilot Channel was constructed to address hypersalinity concerns that resulted from natural and anthropogenically-caused tidal isolation since the early 1940’s. In 2022, the Texas General Land Office (GLO) increased the channel’s width and depth in anticipation of increasing tidal exchange ratios by a factor of two to three. Once the channel widening was complete, two SL-1500-3G side-looking acoustic doppler current profilers (ADCPs) were periodically deployed beneath the …

Long-Term Spatial And Temporal Assessment Of Criteria Air Pollutants Concentration Trends In The Lower Rio Grande Valley Region Of Texas, Usa, Md Salahuddin Majumder

Theses and Dissertations

Air pollution is a substantial environmental challenge all over the world. This study investigated the long-term air pollution levels in the Lower Rio Grande Valley region, by utilizing data collected from five continuous ambient monitoring stations in two counties over 23 years, from January 2000 to December 2022, to analyze daily, monthly, and seasonal variations of different pollutants, including ground-level ozone, particulate matter, and carbon monoxide. This study also identified distinct daily and seasonal patterns for O3, PM2.5, PM10, and CO at each monitoring site, influenced by factors such as traffic emissions, temperature, solar radiation, indoor heating, and weather conditions. …

Conjunctive Management For Groundwater-Surface Water Resources: Numerical Modeling And Potential Assessment Of Managed Aquifer Recharge (Mar) At Lower Rio Grande Valley In South Texas, Dwight Zedric Q. Capus

Theses and Dissertations

Texas is well known to frequent floods and droughts, and yet has mounting concerns in very imbalanced water availability and water demands from growth in population and economical activities. Capturing and storing water is essential to long-term and climate-smart management of the “already-scarce” water resources. This study aimed to apply numerical modeling to evaluate potentials and impacts of Managed Aquifer Recharge (MAR) to regional groundwater systems in the Lower Rio Grande Valley. Using Visual MODFLOW Flex 3D package, different scenarios were simulated for groundwater flow patterns and storage capacities. Water injections tested ranged between 7.30x105 ft3 to 3.65x109 ft3 , …

Salas Y Gómez Benthic Habitat And Community Assessment Of Seamounts And Oceanic Islands, Kara Eckley

Theses and Dissertations

Seamounts are essential benthic habitats that are ecologically important, providing habitat and functioning as stepping stones for dispersal and possible refugia, and economically valuable, supporting deep-sea fisheries and potential mineral mining activities. The Salas y Gómez Ridge in the southeast Pacific Ocean longitudinally contains dozens of seamounts that extend ~2,900 km across the South Pacific Subtropical Gyre. The ridge contains a range of oxygen and nutrient conditions that, along with the distance between seamounts, have led to high levels of endemism, reported in the few studies that have been conducted on seamounts of the region; this study is the first …

Investigating The Relationship Between Dissolved Oxygen And Nitrate Concentration As A Proxy For Marine Ecosystem Health, Natalia M. Moore

Theses and Dissertations

Coastal environments such as seagrass meadows span a wide range of spatial scales and can experience disturbances that cause rapid shifts to ecosystem dynamics. Increased pressure to the coastal zone has highlighted the need for constant, real-time monitoring to monitor current ecosystem status. A custom, low cost, high resolution, in situ sensor network was designed and tested in the Lower Laguna Madre, Texas to characterize primary productivity in seagrass meadows through gradient patterns of dissolved oxygen concentration, with the purpose of determining whether seagrass or phytoplankton were the more dominant primary producer. There was a distinct vertical and horizontal gradient …

Weather Parameters Influencing The Incidence Of Citrus Canker Caused By Aw Strain In The Rio Grande Valley, Amit Sharma

Theses and Dissertations

Citrus canker caused by bacterium Xanthomonas citri subsp. citri (Xcc) seriously affects the citrus industry by making the fruit unmarketable due to unsightly lesions on the fruit. Canker caused by Aw strain of Xcc was reported in the citrus trees located in the residential areas of the Rio Grande Valley (RGV). Canker severity differs amongst cultivars/varieties, and it is influenced by prevailing environmental conditions. Multiple regression modeling of the disease incidence with the environmental variables such as temperature, humidity, windspeed, wind gust, and rainfall was performed to understand the environmental conditions that are favorable for spread of citrus …

Fish Community Analysis Using Multidirectional Rov Video Surveys In The Northwestern Gulf Of Mexico, Keegan J. Angerer

Theses and Dissertations

In this study, ROV surveys with multidirectional video were used to analyze the fish communities associated with artificial reef patches in the Rio Grande Valley artificial reef 13.7 km off the coast of South Padre Island, TX. Nine configurations of reef patches consisting of varying combinations and densities of concrete pyramid and low-profile modules were surveyed. The highest species diversity was found at patches with large deployments of both pyramids and low-profile modules. Total Red Snapper Lutjanus campechanus abundance did not differ between configurations, but the highest abundances of juvenile Red Snapper were found at configurations with one pyramid and …

Red Drum (Sciaenops Ocellatus) Trophic Web Reconstruction Using Stable Isotopes In Two Systems In The Northwestern Gulf Of Mexico, Elizabeth Mogus Garcia

Theses and Dissertations

Estuaries act as nurseries for many important fishes, including predators like Red Drum (Sciaenops ocellatus). Using gut content and stable isotope analyses to better understand juvenile S. ocellatus’ diet and role within the trophic web, a full year of data was collected on a quarterly basis to illustrate a change in diet based on resource availability at two study sites and for two non-overlapping S. ocellatus sizes. Panopeidae and Penaeidae were the most abundant prey items found in S. ocellatus stomachs for both sizes, sites, and four quarters representing over 50% of the diet. Stable isotope analyses …

Network Analysis Of Scientific Research In The Gulf Of Mexico, Juliet S. Vallejo

Theses and Dissertations

The Gulf of Mexico ecosystem represents a significant management challenge in its 3,423-mile coastline and transnational regulatory setting, along five states from the United States, six states from Mexico, and Cuba which borders the southeastern quadrant. Providing various resources to the regional economies, the continued success of these resources depends on the collaboration among transnational participants in bringing together complementary skills and multidisciplinary approaches to producing, circulating, and utilizing scientific knowledge. Using bibliometric analysis of Gulf of Mexico-related published research over 18 years from 2000 to 2018 allows for identifying organizations, their connections, and trends in the production of scientific …

Growth And Reproduction In Gulf Of Mexico Black Corals (Antipatharians) In Field And Laboratory Studies, Victoria E. Salinas

Theses and Dissertations

Black corals provide an important ecosystem of marine life and are found throughout all the oceans of the world at depths between 2 and 8,600 m. However, little is understood about their life history and the factors that control the distribution of black corals, particularly in the Gulf of Mexico (GoM). Given the impacts of the Deepwater Horizon oil spill on soft corals (e.g., black corals and octocorals) in the GoM, studies of their growth and reproductive biology in both natural and aquaculture environments are crucial for restoration efforts. The objectives of this study were to examine reproductive processes, compare …

Effects Of Nutrient Enrichment On Mangrove And Saltmarsh Habitats, Elena A. Flores

Theses and Dissertations

Nutrient over-enrichment is a global threat to coastal and marine environments. Yet, system-specific attributes of estuarine and coastal ecosystems may result in large differences in their sensitivity and susceptibility to such threat. In tropical latitudes, plant species adapted to low-nutrient habitats generally have nutrient-conservation mechanisms that acquire, use, and internally recycle nutrients efficiently. Thus, a major challenge for managers and decision makers is to develop a predictive and holistic understanding of how nutrient loading alters ecosystem functions and therefore ecosystem services. The objective of the present research is to document short and long-term effects of persistent nutrient …

Analyzing The Impact Of Governance Strategies On Trust And Risk In The Salish Sea Transboundary Fishery Context, Evelyn Roozee

Theses and Dissertations

The Salish Sea is the site of a transboundary fishery whose coastal jurisdiction includes British Columbia, Washington State, the two federal governments, and many Indigenous tribes with sovereign rights. Fishery management becomes increasingly complex when transboundary cooperation is needed. Furthermore, while the Salish Sea region has attempted to facilitate better transboundary collaborative governance, these have generally failed to institutionalize the principles of adaptive management. This research seeks to assess current trust and risk perceptions and analyze the effects of control mechanisms used in the transboundary fishery management network. The data consists of a survey measuring collaborative precursors, barriers, and outcomes …

Snook (Centropomus Undecimalis) Behavior And Accelerometer Telemetry In The Bahia Grande Of Texas, Jonathan Truett Cawlfield

Theses and Dissertations

There has been very little study of the Texas population of snook. No studies have have looked at behavior in the Texas snook population. In this study we look at behavioral patterns and activity level in Lower Laguna Madre snook utilizing accelerometer telemetry tags. Twenty-one common snook were surgically implanted with tags and monitored from summer and fall of 2019 until September 2020. The accelerometer tags which sum acceleration over the x, y, and z planes calculated (ODBA) which was used to estimate behavior. This behavior was then compared with environmental data like diel period, tidal period, water temperature, and …

Assessment And Monitoring Of Deltaic Wetlands And Fluvial Systems: Refining And Validating A Multimetric Index Of Resaca Ecosystem Health, David Lecusay Jr.

Theses and Dissertations

Resacas are ancient distributary channels of the lower Rio Grande River historically serving as floodwater drainage pathways onto the Laguna Madre, Texas. Resacas provide numerous ecosystem services and critical habitat for many species. Mainly influenced by urbanization, integrating a multimetric index for assessing and monitoring resaca ecosystem health is required for adaptive management. The Resaca Health Index (RHI) integrates structural and functional components: (1) nonredundant metrics describing fish communities (Fish Community Index); (2) Trophic State Index derived from Secchi depth; (3) decay constant from riparian vegetation leaf litter decomposing in the water column; and (4) a riparian quality index based …

Managing Water Resource And Land Use In Lower Rio Grande Valley Of South Texas Using A Groundwater Vulnerability Model, Leslie Soto Sanchez

Theses and Dissertations

The Lower Rio Grande Valley (LRGV) has three Metropolitan Statistical Areas. These are Brownsville-Harlingen, Laredo, and McAllen-Edinburg-Mission, the latter with a population of around 1 million people and had an estimated growth of 62% from the period of 2010-2018 (Texas State Data Center, 2020). Low precipitations, intensive agricultures as well as growth in the manufacturing and tourism industries have resulted in an increasing concern of scarce water supply especially the region relies primarily on a single water source, the Rio Grande River. These circumstances could potentially disrupt economic development, negatively affecting the local manufacturing industry, agriculture, and the community. With …

Assessing Long-Term Coral Coverage At East And West Flower Garden Banks, Rebekah Alicia Hernandez

Theses and Dissertations

Coral reefs provide critical habitat for diverse benthic communities; however, more than 10% of existing reefs have been lost as a result of natural and anthropogenic stressors. While there has been a decline in reefs around the world, East and West Flower Garden Banks (FGBs), part of Flower Garden Banks National Marine Sanctuary (FGBNMS), have maintained > 50% live coral cover for over 27 years as revealed by the FGBNMS long-term monitoring (LTM) program. In the 1980’s, the LTM program at East and West FGBs was developed in response to the expansion of oil and gas exploration, anchoring, and fishing impacts …

Biochar Applications To The Remediation Of Lead And Polychlorinated Biphenyl Contaminated Water, Victory Itua Igberase

Theses and Dissertations

This study investigated the performance of biochar materials as adsorbents to remove Pb and polychlorinated biphenyl (PCB) in aqueous solutions. Batch adsorption and column experiment packed with biochar and geomaterials (pumice) in 1:1 volume ratio was evaluated to address the efficacy of biochar in removing Pb while only batch adsorption was employed for PCB removal with biochar materials. Overall, PT biochars performed the best (42–53 mg g−1) followed by BE biochars (10–32 mg g−1) and AB biochars (17–27 mg g−1) while high temperature biochars were favored in terms of maximum Pb adsorption capacity. Column Pb breakthrough showed similar results, with …

Investigation Of Techniques And Their Application For The Cryopreservation Of Algal Species, Jazmine Leija

Theses and Dissertations

An alternative source for petroleum-based crude oils are algae derived biofuels. Fossil fuels are harmful for the environment, expensive and becoming scarce. There has been an increase in research on environmentally sustainable energy, using lipids derived from microalgae, which can be converted into biofuel. The use of microalgae as a source for biofuels has a lot of benefits including decreasing greenhouse gas emissions, rapid fuel production, absorption of carbon dioxide, and production of a renewable source of energy.

Algae cryopreservation aids in the maintenance of the best algae strains selected for producing lipids for biofuels. Cryopreservation will help minimize genetic …

Effects Of Elevated Temperature On 8-Hydroxy-2'-Deoxyguanosine Expression, Dna Damage And Cellular Apoptosis In The American Oyster (Crassostrea Virginica), Md Faizur Rahman

Theses and Dissertations

Global temperature increases due to anthropogenic activities. The effects of rising temperature are well documented in aquatic organisms. The American oyster is an ideal shellfish species to study on global warming and oxidative DNA damage. In this study, We observed the effects of high temperature on heat shock protein-70 (HSP70), 8-hydroxy-2’-deoxyguanosine (8-OHdG), ssDNA, dsDNA, caspase-3 (CASP3), BAX and γH2AX, a molecular marker of DNA damage, protein expressions in gills of oysters. Immunohistochemical results showed that elevated temperatures (28 and 32°C) significantly increased HSP70, 8-OHdG, dsDNA, γH2AX, BAX and CASP3 protein expressions in gills of oysters compare to control (24°C). In …

Effects Of Seasonal Variations And Heat Exposure On Antioxidant Expression And Redox Status In The American Oyster, Crassostrea Virginica: Field And Laboratory Studies, Md Sadequr Rahman

Theses and Dissertations

Increasing atmospheric temperatures influence physiological functions in aquatic organisms. In this study, I observed the effects of seasonal (field study) and laboratory heat exposure (16, 22, 26, and 30°C for 1-week) on prooxidant-antioxidant homeostasis in gills and digestive glands of American oyster. Histological analysis showed an increase in mucus secretion with elevated temperatures in both organs. Immunohistochemical analysis showed a significant increase of heat shock protein-70, dinitrophenyl, and 3-nitrotyrosine protein expressions with higher seasonal and laboratory temperatures indicating heat-induced oxidative and nitrosative stress. This was concomitant with gradual rise in cellular apoptosis. Interestingly, catalase and superoxide dismutase expressions also increased …

Development And Assessment Of An Environmental Dna (Edna) Assay For The Rio Grande Siren And Review Of Edna Metabarcoding Applications, Krista M. Ruppert

Theses and Dissertations

Environmental DNA (eDNA) assays have become a major aspect of amphibian surveys in the past decade. These methods are highly sensitive, making them well-suited for monitoring rare and cryptic species. Current efforts to study the Rio Grande Siren in south Texas have been hampered due to the cryptic nature of these aquatic salamanders. Arid conditions further add to the difficulty in studying this species, as many water bodies they inhabit are ephemeral, sometimes constraining sampling efforts to a short window after heavy rain. Additionally, sirens are known to cease activity and reside underground when ponds begin to dry or as …

Analysis Of The Facilitative Interaction Between Batis Maritima And Avicennia Germinans As A Mangrove Restoration Strategy, Javier R. Navarro

Theses and Dissertations

The multiple experiments conducted in an Avicennia germinans forest in Laguna Vista, TX was focused on determining if facilitation theory could explain the behavior observed between the herbaceous halophyte Batis maritima and A. germinans. I hypnotized that a positive interaction exists but through which mechanisms of facilitation I did not know. The overarching theme of the study is the presence or absence of B. maritima. Facilitation theory tells us that positive interactions are most often found under stressful conditions such as those on the coastal tidal flat of the Laguna Madre. Locally B. maritima has been found to …

Detection And Enumeration Of Bacterial Pathogens In The American Oyster (Crassostrea Virginica), Mohammad Maruf Billah

Theses and Dissertations

American oyster (Crassostrea virginica) is a popular seafood for its delicacy and high nutritional value. Based on increasing concern about contamination of bacterial pathogens in raw oyster, my research objectives have been focused on detection and enumeration of two important bacterial pathogens, Escherichia coli and Salmonella spp. in the American oyster in south Texas waters, local markets and controlled laboratory studies. Immunohistochemical and RT-PCR analyses showed substantial bacterial pathogen’s presence in gills and digestive glands of oysters collected from San Martin Lake and South Padre Island as well as local markets. Laboratory studies showed increasing trend of both …

Effects Of Artificial Substrates On The Recruitment Of Juvenile Fishes In The Northwestern Gulf Of Mexico, Stefany Salinas

Theses and Dissertations

Most artificial reefs (ARs) in the Gulf of Mexico are considered intermediate to high vertical relief structures which have recruited large predatory species indicating lack of juvenile habitat. Small, inexpensive ARs, standard monitoring units for the recruitment of reef fish (SMURFs), were deployed at eight sites at -20m as habitat for juvenile fishes to understand and determine the effects of substrate type on juvenile fishes recruitment. Each site contained four SMURFs with four treatments: small and large rubble (~10 and ~20cm), oyster shells, and bare. Surveys yielded 39 juvenile species, including one of the most economically important species, Lutjanus campechanus, …

Enabling High Quality Oxygen Measurements During Robotic Based Studies Of Ocean Ecological And Biogeochemical Processes, Brianna A. Alanis

Theses and Dissertations

Dissolved oxygen is an essential parameter necessary for understanding marine ecological and biogeochemical processes. New robotic vehicles and autonomous platforms are being applied to an even wider range of ecological and biogeochemical studies. Thus, arises the opportunity for matching the best possible oxygen sensing techniques and methods to these new platforms. In so doing, we can enable both more targeted and higher resolution oxygen measurements than previously possible and potentially use oxygen measurements for a wider range of applications, including in situ incubation experiments and primary productivity measurements. This thesis tested three different oxygen sensors in a trade study for …

The Role Of Sediment Resuspension In Estuarine Inorganic Nutrient Cycling, Kirsten Jo Ayres

Theses and Dissertations

Time-scaling of estuarine inorganic nitrogen cycling contains many assumptions due to biogeochemical interactions. Nitrogen, often a limiting factor for primary production, is transformed and utilized by many estuarine organisms. Inorganic nitrogen is especially high in porewater. High nutrient pore water, contained within the interstitial spaces of sediment, has been assumed to influx high concentrations of inorganic nutrients into surface waters during resuspension events. These short-term resuspension events rapidly introducing high concentration of nutrients into the water column. In order to determine the internal time scale of inorganic nitrogen cycling, a box-model nutrient budget, horizontal in situ transects, and vertical nutrient …

Histological Evidence Of Annual And Lunar Reproductive Rhythms Of Atlantic Sea Urchin, Arbacia Punctulata In The Gulf Of Mexico: Changes In Nutritive Phagocytes In Relation To Gametogenesis, Eleazar Hernandez Ii

Theses and Dissertations

Environmental phenomena such as temperature, photoperiod, tidal cycle, and lunar rhythm act as external cues that stimulate the reproductive activity of marine organisms. In this study, I report the annual and lunar reproductive cycles, and changes in nutritive phagocytes (NPs) in relation to gonadal maturation of Atlantic sea urchin (Arbacia punctulata, a primeval species). Monthly and weekly changes in gonadal development/maturation were observed histologically. In male, the testicular lobules were densely packed with sperm from June to August. In female, on the other hand, mature eggs first appeared in some ovaries in May, numerically increased from June to …

A Sinking Feeling: The Fate Of Concrete Pyramids Deployed In Artificial Reefs In The Texas Gulf Of Mexico, Cameron J. Moody

Theses and Dissertations

The deployment of 2,611 concrete pyramids in Texas gulf waters represents a significant undertaking. In collaboration with Texas Parks and Wildlife, bathymetric sidescan data was collected at three reefs hit by Hurricane Harvey from 2017 to 2019. All reefs lost significant pyramid height between 2017 and 2019. The pyramids demonstrated greater subsidence in the year when Harvey occurred and minimal subsidence the following year. Pyramids that moved outside the reefs were due to hurricane forces and shrimp trawl activity. Pyramids that remained inside the reef remained at their deployment location for two reefs. The pyramids remaining in the third reef …