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Full-Text Articles in Physical Sciences and Mathematics

Spatial And Temporal Trends In The Xestospongia Muta (Giant Barrel Sponge) Population On The Southeast Florida Reef Tract, Alanna D. Waldman Jul 2019

Spatial And Temporal Trends In The Xestospongia Muta (Giant Barrel Sponge) Population On The Southeast Florida Reef Tract, Alanna D. Waldman

HCNSO Student Theses and Dissertations

Xestospongia muta, the giant barrel sponge, is a key component of coral reef benthic communities in Southeast Florida and the Caribbean. Xestospongia muta increases habitat complexity and stability, and filters large volumes of water, enhancing water quality and facilitating nutrient cycling. Therefore, it is important to investigate trends in the X. muta population on Southeast Florida reefs in response to anthropogenic stressors, changing environmental conditions and acute disturbances and how these events affect its ecological role in the benthic community. This study identified trends in X. muta population density, volume, and size class distribution over time and across reef …


Past, Present, And Future Perspectives Of Environmental Dna (Edna) Metabarcoding: A Systematic Review In Methods, Monitoring, And Applications Of Global Edna, Krista M. Ruppert, Richard J. Kline, Md Saydur Rahman Jan 2019

Past, Present, And Future Perspectives Of Environmental Dna (Edna) Metabarcoding: A Systematic Review In Methods, Monitoring, And Applications Of Global Edna, Krista M. Ruppert, Richard J. Kline, Md Saydur Rahman

School of Earth, Environmental, and Marine Sciences Faculty Publications and Presentations

Environmental DNA (eDNA) metabarcoding is a novel method of assessing biodiversity wherein samples are taken from the environment via water, sediment or air from which DNA is extracted, and then amplified using general or universal primers in polymerase chain reaction and sequenced using next-generation sequencing to generate thousands to millions of reads. From this data, species presence can be determined, and overall biodiversity assessed. It is an interdisciplinary method that brings together traditional fieldbased ecology with in-depth molecular methods and advanced computational tools. As an emerging monitoring method, there are many pitfalls and roadblocks to be considered and avoided, but …