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Physical Sciences and Mathematics Commons

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Full-Text Articles in Physical Sciences and Mathematics

Screening Genes For Association With Loci For Nitrogen-Use Efficiency In Perennial Ryegrass By PyrosequencingTm, O. Dolstra, D. Dees, J. -D. Driesprong, E. N. Van Loo Mar 2023

Screening Genes For Association With Loci For Nitrogen-Use Efficiency In Perennial Ryegrass By PyrosequencingTm, O. Dolstra, D. Dees, J. -D. Driesprong, E. N. Van Loo

IGC Proceedings (1993-2023)

The application of marker-assisted selection to improve quantitative traits in perennial ryegrass (Lolium perenne) is cumbersome. It requires a priori knowledge on the association of markers and genes. The knowledge on the chromosomal location of major genes for quantitative traits as well as on gene sequences is rapidly growing. However, determination of the genetic constitution of parents prior to their use in breeding still is impractical. More realistic is to collect association data along with the testing activities needed for breeding new varieties. This study uses changes in allele frequency due to selection as a criterion for gene-trait …


Machine Learning Analysis Of Single Nucleotide Polymorphism (Snp) Data To Predict Bone Mineral Density In African American Women, Erick Githua Wakayu Dec 2021

Machine Learning Analysis Of Single Nucleotide Polymorphism (Snp) Data To Predict Bone Mineral Density In African American Women, Erick Githua Wakayu

UNLV Theses, Dissertations, Professional Papers, and Capstones

Osteoporosis is a debilitating disease in which an individual’s bones weaken, making bones fragile and more susceptible to fracture. While commonly found amongst postmenopausal Caucasian and Asian women based on previous studies, those of African descent (African American/Black) have largely been ignored when it comes to osteoporotic studies, especially when it comes to Genome Wide Association Studies (GWAS). From GWA studies, we gain access to single nucleotide poly-morphisms (SNPs) that may contribute to certain illnesses, such as osteoporosis. With low Bone Mineral Density (BMD) being one of the primary markers of potential osteoporosis, it is prudent that proper research is …


Molecular Markers For Classification Of Seed Collections Of Lolium Cultivars, Maragret Wallace, J. Provan, Trevor J. Gilliland Apr 2021

Molecular Markers For Classification Of Seed Collections Of Lolium Cultivars, Maragret Wallace, J. Provan, Trevor J. Gilliland

IGC Proceedings (1993-2023)

No abstract provided.


Is There Genetic Diversity In The ‘Leucaena Bug’ Synergistes Jonesii Which May Reflect Ability To Degrade Leucaena Toxins?, Jagadish Padmanabha, Michael J. Halliday, Stuart E. Denman, Carl K. Davis, H. Max Shelton, Chris S. Mcsweeney Feb 2020

Is There Genetic Diversity In The ‘Leucaena Bug’ Synergistes Jonesii Which May Reflect Ability To Degrade Leucaena Toxins?, Jagadish Padmanabha, Michael J. Halliday, Stuart E. Denman, Carl K. Davis, H. Max Shelton, Chris S. Mcsweeney

IGC Proceedings (1993-2023)

Leucaena leucocephala, a nutritionally rich forage tree legume, contains a non-protein amino acid, mimosine, which is degraded by ruminal bacteria to toxic metabolites 3,4-DHP and 2,3-DHP resulting in goitre-like symptoms in animals, severely restricting weight gain. Raymond Jones, in the early 1980s, discovered the ‘leucaena bug’ in the rumen of goats in Hawaii that degraded these toxic DHP metabolites into non-toxic compounds (Jones and Lowry 1984) which was named Synergistes jonesii (Allison et al. 1992) Subsequently, a rumen inoculum containing S. jonesii was used as an ‘oral drench’ for cattle, kept in continuous culture (Klieve et al. …


Designing Computational Biology Workflows With Perl - Part 2, Esma Yildirim May 2019

Designing Computational Biology Workflows With Perl - Part 2, Esma Yildirim

Open Educational Resources

This material introduces the AWS console interface, describes how to create an instance on AWS with the VMI provided and connect to that machine instance using the SSH protocol. Once connected, it requires the students to write a script to automate the tasks to create VCF files from two different sample genomes belonging to E.coli microorganisms by using the FASTA and FASTQ files in the input folder of the virtual machine. The same exercise can be applied if the VMI is installed on a local machine using virtualization software (e.g. Oracle VirtualBox). In this case, the Terminal program of the …


Designing Computational Biology Workflows With Perl - Part 2, Esma Yildirim May 2019

Designing Computational Biology Workflows With Perl - Part 2, Esma Yildirim

Open Educational Resources

This material briefly reintroduces the DNA double Helix structure, explains SNP and INDEL mutations in genes and describes FASTA, FASTQ, BAM and VCF file formats. It also explains the index creation, alignment, sorting, marking duplicates and variant calling steps of a simple preprocessing workflow and how to write a Perl script to automate the execution of these steps on a Virtual Machine Image.