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Full-Text Articles in Physical Sciences and Mathematics
Dynamic Supplying Desired Web Service For E-Business, Xuejuan Huang, Xinmeng Chen, Jiazhen Xu, Jie Yang
Dynamic Supplying Desired Web Service For E-Business, Xuejuan Huang, Xinmeng Chen, Jiazhen Xu, Jie Yang
Faculty of Science - Papers (Archive)
With the number of available Web services growing large, supplying the appropriate services for e-business becomes crucial. Dynamic change of customer requirement and available service space challenges the service discovery and selection process. In this paper, we propose a Web service dynamic supplying framework, called Web service community (WSC), to facilitate invocation of one single service or the composition of several services to complete various business tasks. The prominent features of Web service description, discovery and selection in the circumstance of WSC are depicted based on the explanation of the structure of WSC. The desired Web services are dynamically discovered …
Stable Isotope Metabolic Labeling With A Novel 15n-Enriched Bacteria Diet For Improved Proteomic Analyses Of Mouse Models For Psychopathologies, Yinglong Zhang, Elisabeth T. Frank, B. Hambsch, C. W. Turck, M. Bunck, R. Landgraf, M. S. Kessler, G. Maccarrone, M. Filiou, Hermann Heumann, Stefan Reckow
Stable Isotope Metabolic Labeling With A Novel 15n-Enriched Bacteria Diet For Improved Proteomic Analyses Of Mouse Models For Psychopathologies, Yinglong Zhang, Elisabeth T. Frank, B. Hambsch, C. W. Turck, M. Bunck, R. Landgraf, M. S. Kessler, G. Maccarrone, M. Filiou, Hermann Heumann, Stefan Reckow
Faculty of Science - Papers (Archive)
The identification of differentially regulated proteins in animal models of psychiatric diseases is essential for a comprehensive analysis of associated psychopathological processes. Mass spectrometry is the most relevant method for analyzing differences in protein expression of tissue and body fluid proteomes. However, standardization of sample handling and sample-to-sample variability are problematic. Stable isotope metabolic labeling of a proteome represents the gold standard for quantitative mass spectrometry analysis. The simultaneous processing of a mixture of labeled and unlabeled samples allows a sensitive and accurate comparative analysis between the respective proteomes. Here, we describe a cost-effective feeding protocol based on a newly …
Development Of Non-Antibiotic-Resistant, Chromosomally Based, Constitutive And Inducible Expression Systems For Aroa-Attenuated Salmonella Enterica Serovar Typhimurium, Jake Matic, Tamsin D. Terry, David Van Bockel, Tracy A. Maddocks, David Tinworth, Michael Jennings, Steven P Djordjevic, Mark J. Walker
Development Of Non-Antibiotic-Resistant, Chromosomally Based, Constitutive And Inducible Expression Systems For Aroa-Attenuated Salmonella Enterica Serovar Typhimurium, Jake Matic, Tamsin D. Terry, David Van Bockel, Tracy A. Maddocks, David Tinworth, Michael Jennings, Steven P Djordjevic, Mark J. Walker
Faculty of Science - Papers (Archive)
Live-vaccine delivery systems expressing two model antigens from Mycoplasma hyopneumoniae, F2(P97) (Adh) and NrdF, were constructed using Salmonella enterica serovar Typhimurium aroA (STM-1), and immunogenicity in mice was evaluated. Recombinant plasmid-based expression (PBE) and chromosomally based expression (CBE) systems were constructed. The PBE system was formed by cloning both antigen genes into pJLA507 to create an operon downstream of temperature-inducible promoters. Constitutive CBE was achieved using a promoter-trapping technique whereby the promoterless operon was stably integrated into the chromosome of STM-1, and the expression of antigens was assessed. The chromosomal position of the operon was mapped in four clones. Inducible …
Gremo: A Gis-Based Generic Model For Estimating Relative Wave Exposure, Austen Pepper, Marjetta L. Puotinen
Gremo: A Gis-Based Generic Model For Estimating Relative Wave Exposure, Austen Pepper, Marjetta L. Puotinen
Faculty of Science - Papers (Archive)
Wave exposure plays a major role in shaping the ecological structure of nearshore communities, with different community types able to survive and/or thrive when typically exposed to different levels of wave energy. This can be quantified by taking direct field measurements with wave buoys over time and then manipulating the data to derive typical conditions. However, taking these measurements is only feasible for very limited areas due to logistical constraints, and generating them with numerical wave models can also be expensive to run and may require data inputs that are either lacking or are highly uncertain. Instead, the relative differences …