Physical Sciences and Mathematics Commons^™

Whole Farm Net Zero: Approaches To Quantification Of Climate Regulation Ecosystem Services At The Whole Farm Scale. Vermont Payment For Ecosystem Services Technical Report #7, Christopher Bonasia, Lindsey Ruhl, Benjamin Timothy Dube, Alissa C. White, Heather M. Darby

UVM Extension Faculty Publications

In this report, approaches to the quantification of climate mitigation ecosystem services at the whole farm scale are reviewed and summarized for easy comparison. Eight quantification tools, and three case studies demonstrating possible tool applications, are summarized to fulfill the requirements of the Technical Services Contract—Task 7. Information from a combination of literature review and expert interviews served to document the inputs, outputs, strengths, weaknesses, opportunities, and threats for each quantification tool. This research was conducted in service to the Vermont Soil Health and Payment for Ecosystem Services (PES) Working Group (VT PES working group). It is our hope that …

Results Of The 2022 Vermont Farmer Conservation & Payment For Ecosystem Services Survey. Vermont Payment For Ecosystem Services Technical Research Report #3a, Alissa C. White

Reports and Policy Briefs

This survey was commissioned by the Vermont Soil Health and Payment for Ecosystem Services Working Group (VT PES Working Group) to gather farmer input on the development of payment for ecosystem services (PES) in Vermont for agriculture. In particular, the survey was intended to help set appropriate levels of compensation for participation in a soil health PES program, although additional information was gathered in the survey to inform the development of a new incentive program. The VT PES Working Group has explored the potential for a performance-based soil health PES program that would compensate farmers on the basis of environmental …

Field Scale Soil Health Scenarios. Vermont Payment For Ecosystem Services Technical Report #2, Alissa C. White, Heather M. Darby, Lindsey C. Ruhl, Bryony Sands, Sara Ziegler,, Juan P. Alvez, Sarah Brickman

UVM Extension Faculty Publications

This report illustrates how changes in management on Vermont farms can influence soil health metrics at the field scale. We’ve used regionally relevant science-based scenarios to demonstrate how selected soil health metrics that are associated with ecosystem services could change on farms in response to management practices at the field scale. These field scale management scenarios demonstrate that many practices in use by farmers in Vermont can have positive impacts on the soil health indicators of interest to the Vermont Soil Health & Payment for Ecosystem Services Working Group. The scenarios document potential for tradeoffs among soil health properties. Specifically, …

Soil Carbon Storage And Sequestration In Vermont Agriculture, Alissa C. White, Heather M. Darby, Donald Ross

UVM Extension Faculty Publications

In 2021, The State of Soil Health (SOSH) project measured indicators of soil health on 221 farm fields across the state of Vermont through a collaborative effort among many organizations. Soil carbon stocks to 30 cm depth were assessed on 191 of those fields. In this brief we share a summary of this new soil carbon stock data alongside data from a national assessment of soil carbon stocks performed by the NRCS from 2010 and highlight its relevance to current policy conversations within the state of Vermont.

Key Ideas

• The protection of existing soil carbon stocks and support for increased …

Tile Drainage Flow Partitioning And Phosphorus Export In Vermont Usa, Ryan Ruggiero, Donald Ross, Joshua W. Faulkner

Lake Champlain Sea Grant Institute

Tile drainage (TD) has been identified as a potential non-point source of phosphorus (P) pollution and subsequent water quality issues. Three fields with TD in Vermont USA were monitored to characterize hydrology and P export. Fields were in corn silage and used minimal tillage and cover cropping practices. Preferential flow path (PFP) activity was explored by separating TD flow into flow pathway and source connectivity components using two hydrograph separation techniques, electrical conductivity end member unmixing, and hydrograph recession analysis. TD was the dominant P export pathway because of higher total discharge. Drought conditions during this study limited surface runoff, …

Measuring Ecosystem Services From Soil Health. Vermont Payment For Ecosystem Services Technical Research Report #1, Alissa C. White, Heather M. Darby, Benjamin Timothy Dube, Bryony Sands, Joshua W. Faulkner, Meredith Albers, Maggie Payne

UVM Extension Faculty Publications

There are a multitude of approaches to evaluating soil health and the soil processes influenced by soil health. As the state of Vermont explores innovative programs that compensate farmers for soil health and associated ecosystem services, the selection of soil health indicators and quantification methods is a foundational first step that influences other aspects of program design. What is measured determines the ecosystem services that can be inferred, the accuracy of data that informs decisions, and programmatic transaction costs. Simply put, what is measured matters. The PES Working Group identified organic matter, bulk density, aggregate stability, greenhouse gas flux from …

Emulating Agricultural Disease Management: Comparing Risk Preferences Between Industry Professionals And Online Participants Using Experimental Gaming Simulations And Paired Lottery Choice Surveys, Eric M. Clark, Scott C. Merrill, Luke Trinity, Gabriela Bucini, Nicholas Cheney, Ollin Langle-Chimal, Trisha Shrum, Christopher Koliba, Asim Zia, Julia M. Smith

College of Agriculture and Life Sciences Faculty Publications

Mitigating the spread of disease is crucial for the well-being of agricultural production systems. Implementing biosecurity disease prevention measures can be expensive, so producers must balance the costs of biosecurity investments with the expected benefits of reducing the risk of infections. To investigate the risk associated with this decision making process, we developed an online experimental game that simulates biosecurity investment allocation of a pork production facility during an outbreak. Participants are presented with several scenarios that vary the visibility of the disease status and biosecurity protection implemented at neighboring facilities. Certain rounds allowed participants to spend resources to reduce …

Awareness Of Climate Change’S Impacts And Motivation To Adapt Are Not Enough To Drive Action: A Look Of Puerto Rican Farmers After Hurricane Maria, Luis Alexis Rodríguez-Cruz, Meredith T. Niles

College of Agriculture and Life Sciences Faculty Publications

Understanding how perceptions around motivation, capacity, and climate change’s impacts relate to the adoption of adaptation practices in light of experiences with extreme weather events is important in assessing farmers’ adaptive capacity. However, very little of this work has occurred in islands, which may have different vulnerabilities and capacities for adaptation. Data of surveyed farmers throughout Puerto Rico after Hurricane Maria (n = 405, 87% response rate) were used in a structural equation model to explore the extent to which their adoption of agricultural practices and management strategies was driven by perceptions of motivation, vulnerability, and capacity as a function …

Mobilizing Crop Biodiversity, Susan Mccouch, Zahra Katy Navabi, Michael Abberton, Noelle L. Anglin, Rosa Lia Barbieri, Michael Baum, Kirstin Bett, Helen Booker, Gerald L. Brown, Glenn J. Bryan, Luigi Cattivelli, David Charest, Kellye Eversole, Marcelo Freitas, Kioumars Ghamkhar, Dario Grattipaglia, Robert Henry, Maria Cleria Valadares Inglis, Tofazzal Islam, Zakaria Kehel, Paul J. Kersey, Graham J. King, Stephen Kresovich, Emily Marden, Sean Mayes, Marie Noelle Ndjiondjiop, Henry T. Nguyen, Samuel Rezende Paiva, Roberto Papa, Peter W.B. Phillips, Awais Rasheed

College of Agriculture and Life Sciences Faculty Publications

No abstract provided.

Land Use And Season Influence Event-Scale Nitrate And Soluble Reactive Phosphorus Exports And Export Stoichiometry From Headwater Catchments, Dustin W. Kincaid, Erin C. Seybold, E. Carol Adair, William B. Bowden, Julia N. Perdrial, Matthew C.H. Vaughan, Andrew W. Schroth

College of Arts and Sciences Faculty Publications

Catchment nutrient export, especially during high flow events, can influence ecological processes in receiving waters by altering nitrogen (N) and phosphorus (P) concentrations and relative amounts (stoichiometry). Event-scale N and P export dynamics may be significantly altered by land use/land cover (LULC) and season. Consequently, to manage water resources, it is important to understand how LULC and season interact to influence event N and P export. In situ, high-frequency spectrophotometers allowed us to continuously and concurrently monitor nitrate (NO3−) and soluble reactive P (SRP) concentrations and therefore examine event-scale NO3− and SRP export dynamics. Here we analyzed event NO3− and …

Land Use And Season Influence Event-Scale Nitrate And Soluble Reactive Phosphorus Exports And Export Stoichiometry From Headwater Catchments, Dustin W. Kincaid, Erin C. Seybold, E. Carol Adair, William B. Bowden, Julia N. Perdrial, Matthew C.H. Vaughan, Andrew W. Schroth

Rubenstein School of Environment and Natural Resources Faculty Publications

Catchment nutrient export, especially during high flow events, can influence ecological processes in receiving waters by altering nitrogen (N) and phosphorus (P) concentrations and relative amounts (stoichiometry). Event-scale N and P export dynamics may be significantly altered by land use/land cover (LULC) and season. Consequently, to manage water resources, it is important to understand how LULC and season interact to influence event N and P export. In situ, high-frequency spectrophotometers allowed us to continuously and concurrently monitor nitrate (NO3−) and soluble reactive P (SRP) concentrations and therefore examine event-scale NO3− and SRP export dynamics. Here we analyzed event NO3− and …

Genome-Wide Association Study In Accessions Of The Mini-Core Collection Of Mungbean (Vigna Radiata) From The World Vegetable Gene Bank (Taiwan), Alena Sokolkova, Marina Burlyaeva, Tatjana Valiannikova, Margarita Vishnyakova, Roland Schafleitner, Cheng Ruei Lee, Chau Ti Ting, Ramakrishnan Madhavan Nair, Sergey Nuzhdin, Maria Samsonova, Eric Von Wettberg

College of Agriculture and Life Sciences Faculty Publications

Background: Mungbean (Vigna radiata (L.) R. Wilczek, or green gram) is important tropical and sub-tropical legume and a rich source of dietary protein and micronutrients. In this study we employ GWAS to examine the genetic basis of variation in several important traits in mungbean, using the mini-core collection established by the World Vegetable Center, which includes 296 accessions that represent the major market classes. This collection has been grown in a common field plot in southern European part of Russia in 2018. Results: We used 5041 SNPs in 293 accessions that passed strict filtering for genetic diversity, linkage disequilibrium, population …

Dynamical Climatic Model For Time To Flowering In Vigna Radiata, Konstantin Kozlov, Alena Sokolkova, Cheng Ruei Lee, Chau Ti Ting, Roland Schafleitner, Eric Bishop-Von Wettberg, Sergey Nuzhdin, Maria Samsonova

College of Agriculture and Life Sciences Faculty Publications

Background: Phenology data collected recently for about 300 accessions of Vigna radiata (mungbean) is an invaluable resource for investigation of impacts of climatic factors on plant development. Results: We developed a new mathematical model that describes the dynamic control of time to flowering by daily values of maximal and minimal temperature, precipitation, day length and solar radiation. We obtained model parameters by adaptation to the available experimental data. The models were validated by cross-validation and used to demonstrate that the phenology of adaptive traits, like flowering time, is strongly predicted not only by local environmental factors but also by plant …

Editorial: Wild Plants As Source Of New Crops, Eric Von Wettberg, Thomas M. Davis, Petr Smýkal

College of Agriculture and Life Sciences Faculty Publications

No abstract provided.

Benefits And Tradeoffs Of Reduced Tillage And Manure Application Methods In A Zea Mays Silage System, Kyle M. Dittmer, Heather M. Darby, Tyler R. Goeschel, E. Carol Adair

College of Agriculture and Life Sciences Faculty Publications

A critical question is whether there are agricultural management practices that can attain the multiple management goals of increasing yields, preventing nutrient losses, and suppressing greenhouse gas (GHG) emissions. No-till and manure application methods, such as manure injection, can enhance nutrient retention, but both may also enhance emissions of nitrous oxide (N2O), a powerful GHG. We assessed differences in soil N2O and carbon dioxide (CO2) emissions, nitrate and ammonium retention, and crop yield and protein content under combinations of vertical-till, no-till, manure injection, and manure broadcast without incorporation in a corn (Zea mays L.) silage system. During the growing seasons …

Perspective Article: Actions To Reconfigure Food Systems, Ana Maria Loboguerrero, Philip Thornton, Jonathan Wadsworth, Bruce M. Campbell, Mario Herrero, Daniel Mason-D'Croz, Dhanush Dinesh, Sophia Huyer, Andy Jarvis, Alberto Millan, Eva Wollenberg, Stephen Zebiak

Rubenstein School of Environment and Natural Resources Faculty Publications

There is broad agreement that current food systems are not on a sustainable trajectory that will enable us to reach the Sustainable Development Goals by 2030, particularly in the face of anthropogenic climate change. Guided by a consideration of some food system reconfigurations in the past, we outline an agenda of work around four action areas: rerouting old systems into new trajectories; reducing risks; minimising the environmental footprint of food systems; and realigning the enablers of change needed to make new food systems function. Here we highlight food systems levers that, along with activities within these four action areas, may …

Making Hemp Choices: Evidence From Vermont, Jane Kolodinsky, Hannah Lacasse, Katherine Gallagher

College of Agriculture and Life Sciences Faculty Publications

Hemp's reemergence in the United States' economy presents an opportunity for a new category of sustainable product choice for consumers. This study fills a gap in knowledge about which consumers are currently aware of or choosing hemp products using the theory of choice alternatives and a statistically representative survey from a top ten hemp producing US state. Findings reveal high levels of consumer awareness and consideration of hemp products in general and a smaller evoked/choice set. Cannabidiol products appear most often in our sample's choice set; we examined these specifically. Other hemp products also appear, including clothing and textile, personal …

Evaluation Of Nitrogen And Phosphorus Removal From A Denitrifyingwoodchip Bioreactor Treatment System Receiving Silage Bunker Runoff, Jillian C. Sarazen, Joshua W. Faulkner, Stephanie E. Hurley

College of Agriculture and Life Sciences Faculty Publications

Leachate and storm-driven runoff from silage storage bunkers can degrade receiving water bodies if left untreated. This study evaluated a novel treatment system consisting of three treatment tanks with a moving-bed biofilm reactor and paired side-by-side denitrifying woodchip bioreactors for the ability to reduce influent nutrient mass loads. Flow-based samples were taken at four locations throughout the system, at the inflow to the first tank, outflow from the tanks prior to entering the woodchip bioreactors, and from the outflows of both bioreactors. Samples were analyzed for concentrations of nitrogen (N) and phosphorus (P) species. Inflow concentrations were reduced from the …

Legume Genetics And Biology: From Mendel’S Pea To Legume Genomics, Petr Smýkal, Eric J.B. Von Wettberg, Kevin Mcphee

College of Agriculture and Life Sciences Faculty Publications

Legumes have played an important part in cropping systems since the dawn of agriculture, both as human food and as animal feed. The legume family is arguably one of the most abundantly domesticated crop plant families. Their ability to symbiotically fix nitrogen and improve soil fertility has been rewarded since antiquity and makes them a key protein source. The pea was the original model organism used in Mendel’s discovery of the laws of inheritance, making it the foundation of modern plant genetics. This Special Issue provides up-to-date information on legume biology, genetic advances, and the legacy of Mendel.

Growing Specialty Coffee: Economic Security And Environmental Sustainability In Global Coffee Systems, Stephen Posner, Janica Anderzen, Alejandra Guzman Luna, Ernesto Mendez

Reports and Policy Briefs

The Gund Institute has partnered with the Agroecology & Livelihoods Collaborative to create a more sustainable and just global coffee sector. Gund Fellows used this research brief to leverage our networks and engage NGOs such as The Specialty Coffee Association, funders, and companies in early discussions about how to address systemic inequity across the coffee value chain.

Green And Animal Manure Use In Organic Field Crop Systems, Patrick M. Carr, Michel A. Cavigelli, Heather Darby, Kathleen Delate, Jed O. Eberly, Heather K. Fryer, Greta G. Gramig, Joseph R. Heckman, Ellen B. Mallory, Jennifer R. Reeve, Erin M. Silva, David H. Suchoff, Alex L. Woodley

College of Agriculture and Life Sciences Faculty Publications

Dual-use cover/green manure (CGM) crops and animal manure are used to supply nitrogen (N) and phosphorus (P) to organically grown field crops. A comprehensive review of previous research was conducted to identify how CGM crops and animal manure have been used to meet N and P needs of organic field crops, and to identify knowledge gaps to direct future research efforts. Results indicate that: (a) CGM crops are used to provide N to subsequent cash crops in rotations; (b) CGM-supplied N generally can meet field crop needs in warm, humid regions but is insufficient for organic grain crops grown in …

Agricultural Biosecurity: Reducing Risks And Impacts Of Livestock Diseases, Stephen Posner, Scott Merrill, Julie Smith

Reports and Policy Briefs

Gund Fellows co-developed timely, innovative research to reduce the risk of animal diseases like coronaviruses spreading among farms. This policy brief helped to inform decision makers at the US Department of Agriculture Foreign Agricultural Service, Department of State Biosecurity Engagement Program, and private sector stakeholders.

Upcycling Phosphorus Recovered From Anaerobically Digested Dairy Manure To Support Production Of Vegetables And Flowers, Katherine K. Porterfield, Robert Joblin, Deborah A. Neher, Michael Curtis, Steve Dvorak, Donna M. Rizzo, Joshua W. Faulkner, Eric D. Roy

College of Engineering and Mathematical Sciences Faculty Publications

Dissolved air flotation (DAF) separates phosphorus (P)-rich fine solids from anaerobically digested dairy manure, creating opportunities to export surplus P to the marketplace as a bagged plant food product. Seedlings of tomato and marigold were amended at various volume per volume (v/v) ratios with plant foods consisting of fine solids upcycled (i.e., transformed into a higher quality product) by drying and blending with other organic residuals. A plate competition assay was conducted to assess the fine solids' potential to suppress the plant pathogen Rhizoctonia solani. Plant foods were comprised of 2.0-2.1% N, 0.8-0.9% P and 0.6-0.8% K. Extractions indicated that …

Agricultural Development Addresses Food Loss And Waste While Reducing Greenhouse Gas Emissions, Gillian L. Galford, Olivia Peña, Amanda K. Sullivan, Julie Nash, Noel Gurwick, Gillian Pirolli, Meryl Richards, Julianna White, Eva Wollenberg

Rubenstein School of Environment and Natural Resources Faculty Publications

Food loss and waste (FLW) reduce food available for consumption and increase the environmental burden of production. Reducing FLW increases agricultural and value-chain productivity and may reduce greenhouse gas emissions associated with feeding the global population. Although studies of interventions that reduce FLW exist, almost no research systematically investigates FLW interventions across multiple value chains or countries, most likely due to challenges in collecting and synthesizing data and estimates, let alone estimating greenhouse gas emissions. Our research team investigated changes in FLW in projects supported by the United States Agency for International Development's (USAID) global hunger and food security initiative, …

How To Measure, Report And Verify Soil Carbon Change To Realize The Potential Of Soil Carbon Sequestration For Atmospheric Greenhouse Gas Removal, Pete Smith, Jean Francois Soussana, Denis Angers, Louis Schipper, Claire Chenu, Daniel P. Rasse, Niels H. Batjes, Fenny Van Egmond, Stephen Mcneill, Matthias Kuhnert, Cristina Arias-Navarro, Jorgen E. Olesen, Ngonidzashe Chirinda, Dario Fornara, Eva Wollenberg, Jorge Álvaro-Fuentes, Alberto Sanz-Cobena, Katja Klumpp

Rubenstein School of Environment and Natural Resources Faculty Publications

Global Change Biology published by John Wiley & Sons Ltd There is growing international interest in better managing soils to increase soil organic carbon (SOC) content to contribute to climate change mitigation, to enhance resilience to climate change and to underpin food security, through initiatives such as international ‘4p1000’ initiative and the FAO's Global assessment of SOC sequestration potential (GSOCseq) programme. Since SOC content of soils cannot be easily measured, a key barrier to implementing programmes to increase SOC at large scale, is the need for credible and reliable measurement/monitoring, reporting and verification (MRV) platforms, both for national reporting and …

Visualizing Climate Change Adaptation: An Effective Tool For Agricultural Outreach?, Rachel E. Schattman, Stephanie E. Hurley, Holly L. Greenleaf, Meredith T. Niles, Martha Caswell

College of Agriculture and Life Sciences Faculty Publications

Landscape photovisualizations (PVZs) are digitally altered photographs that show existing landscapes altered to include a simulated future scenario. They are commonly used to support dialogue and decision-making in multistakeholder contexts. In agricultural sectors, stakeholders increasingly must contend with pressures to adapt to climatic changes and shifts in weather patterns. This study examines the potential of PVZs to engage agricultural stakeholders about climate change adaptation, specifically around best management practices (BMPs). In 2015, survey data were collected (n 5 133) at six agricultural confer-ences Vermont. Participants were asked about their climate change knowledge, perceptions of adaptation, and their intentions to adopt …

Evaluating Water Quality Regulation As A Driver Of Farmer Behavior: A Social-Ecological Systems Approach, Courtney R.Hammond Wagner, Suzie Greenhalgh, Meredith T. Niles, Asim Zia, William B. Bowden

College of Agriculture and Life Sciences Faculty Publications

Water quality policy for agricultural lands seeks to improve water quality by changing farmer behavior. We investigate farmer behavior in three water quality regimes that differ by rule structure to examine the fit and interplay of each policy within its social-ecological context, important aspects for improving water quality. Vermont, USA’s practice-based policy requires the adoption of specific practices, whereas New Zealand’s Lake Taupo and Lake Rotorua performance-based policies require farmers to meet a numeric limit for nutrient loss on their farm. Across the three regions we interviewed 38 farmers to elicit mental models of nutrient management changes. We utilized the …

Making Trees Count: Measurement And Reporting Of Agroforestry In Unfccc National Communications Of Non-Annex I Countries, Todd S. Rosenstock, Andreas Wilkes, Courtney Jallo, Nictor Namoi, Medha Bulusu, Marta Suber, Damaris Mboi, Rachmat Mulia, Elisabeth Simelton, Meryl Richards, Noel Gurwick, Eva Wollenberg

Rubenstein School of Environment and Natural Resources Faculty Publications

Agroforestry—the integration of trees with crops and livestock—generates many benefits directly relevant to the UNFCCC's Koronivia Joint Work on Agriculture, including: (i) building resilience, (ii) increasing soil carbon and improving soil health, (iii) providing fodder and shade for sustainable livestock production and (iv) diversifying human diets and economic opportunities. Despite its significance to the climate agenda, agroforestry may not be included in measurement, reporting and verification (MRV) systems under the UNFCCC. Here we report on a first appraisal of how agroforestry is treated in national MRV systems under the UNFCCC. We examined national communications (NCs) and Nationally Determined Contributions (NDCs) …

Functional Dissection Of The Chickpea (Cicer Arietinum L.) Stay-Green Phenotype Associated With Molecular Variation At An Ortholog Of Mendel’S I Gene For Cotyledon Color: Implications For Crop Production And Carotenoid Biofortification, Kaliamoorthy Sivasakthi, Edward Marques, Ng’Andwe Kalungwana, Noelia Carrasquilla-Garcia, Peter L. Chang, Emily M. Bergmann, Erika Bueno, Matilde Cordeiro, Syed Gul A.S. Sani, Sripada M. Udupa, Irshad A. Rather, Reyazul Rouf Mir, Vincent Vadez, George J. Vandemark, Pooran M. Gaur, Douglas R. Cook, Christine Boesch, Eric J.B. Von Wettberg, Jana Kholova, R. Varma Penmetsa

College of Agriculture and Life Sciences Faculty Publications

“Stay-green” crop phenotypes have been shown to impact drought tolerance and nutritional content of several crops. We aimed to genetically describe and functionally dissect the particular stay-green phenomenon found in chickpeas with a green cotyledon color of mature dry seed and investigate its potential use for improvement of chickpea environmental adaptations and nutritional value. We examined 40 stay-green accessions and a set of 29 BC2F4-5 stay-green introgression lines using a stay-green donor parent ICC 16340 and two Indian elite cultivars (KAK2, JGK1) as recurrent parents. Genetic studies of segregating populations indicated that the green cotyledon trait is controlled by a …

Genotype-Specific Effects Of Ericoid Mycorrhizae On Floral Traits And Reproduction In Vaccinium Corymbosum, Alison K. Brody, Benjamin Waterman, Taylor H. Ricketts, Allyson L. Degrassi, Jonathan B. González, Jeanne M. Harris, Leif L. Richardson

Rubenstein School of Environment and Natural Resources Faculty Publications

American Journal of Botany is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America. Premise: Most plants interact with mycorrhizal fungi and animal pollinators simultaneously. Yet, whether mycorrhizae affect traits important to pollination remains poorly understood and may depend on the match between host and fungal genotypes. Here, we examined how ericoid mycorrhizal fungi affected flowering phenology, floral traits, and reproductive success, among eight genotypes of highbush blueberry, Vaccinium corymbosum (Ericaceae). We asked three overarching questions: (1) Do genotypes differ in response to inoculation? (2) How does inoculation affect floral and flowering traits? (3) Are inoculated …