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Full-Text Articles in Physical Sciences and Mathematics

Modeling The Response Of Black Walnut -Dominant Mixed Hardwoods To Seasonal Climate Effects With Uav-Based Hyperspectral Sensor And Aerial Photogrammetry, Tyler G. Bradford Dec 2018

Modeling The Response Of Black Walnut -Dominant Mixed Hardwoods To Seasonal Climate Effects With Uav-Based Hyperspectral Sensor And Aerial Photogrammetry, Tyler G. Bradford

MSU Graduate Theses

The development of compact sensors in recent years has inspired the use of UAS-based hyperspectral and aerial imaging techniques for small-scale remote sensing applications. With increasing concerns about climate change, spectrally-derived vegetation indices (VIs) have proven useful for quantifying stress-induced vegetation response. The goal of this study was to develop predictive models and assess methodology for modeling the biological response of a black walnut -dominant mixed hardwood stand to seasonal climate events using UAV-based hyperspectral remote-sensing. The derived VIs were evaluated against the means of four seasonal measures of climate calculated for a two-week period prior to the flight date. …


Development Of Rapid, Homogeneous Assay For Investigating Isopeptide Bond Formation Using Fluorescence Polarization/Depolarization Measurements, Samuel Patricc Kasson Aug 2018

Development Of Rapid, Homogeneous Assay For Investigating Isopeptide Bond Formation Using Fluorescence Polarization/Depolarization Measurements, Samuel Patricc Kasson

MSU Graduate Theses

Autocatalytic intramolecular isopeptide bonds have been found in nature in certain gram-positive bacterial pilus structures. Recently, splitting of these domains that are capable of autocatalytic intramolecular isopeptide bond formation have been applied to create stable, selective, bio-orthogonal Catcher/Tag systems. The CnaB2 domain found in the FbaB pilus structure of Streptococcus pyogenes, has yielded the Catcher/Tag, Protein/Peptide systems termed SpyCatchter and SpyTag. Recent study has focused on tag optimization, stability and bio-orthogonality evaluation, along with applications in bioconjugation. I have recombinantly expressed SpyCatcher and SpyTag-fused proteins in E.coli, and conjugated them to fluorescent probes in order for use in fluorescence polarization/depolarization …