Physical Sciences and Mathematics Commons^™

The Synthesis Of Methotrexate-Loaded F127 Microemulsions And Their In Vivo Toxicity In A Rat Model, Abbas Rahdar, Mohammad Reza Hajinezhad, Sarah Nasri, Hamid Beyzaei, Mahmood Barani, John F. Trant

Chemistry and Biochemistry Publications

Methotrexate (MTX) has been often formulated as nano and micro-emulsions, nominally to address its poor solubility and off-target effects. Nanoformulated MTX is universally reported to be a more efficacious anti-cancer agent than direct-dissolved drug; however, these investigations generally fail to screen for in vivo toxicity. This study aims to remedy this oversight. MTX was formulated as a standard Pluronic oil-in-water microemulsion with good drug encapsulation efficiency (73.0% ± 8.4). Preliminary in vitro free radical scavenging studies found that formulation reduces drug oxidation four-fold. The toxic effects of formulated and unformulated MTX were investigated in a Wistar rat model. Rats received …