Physical Sciences and Mathematics Commons^™

Phenotypic Characterization And 16s Rdna Identification Of Culturable Non-Obligate Halophilic Bacterial Communities From A Hypersaline Lake, La Sal Del Rey, In Extreme South Texas (Usa), Kristen Phillips, Frederic Zaidan, Omar R. Elizondo, Kristine L. Lowe

Biology Faculty Publications and Presentations

Background

La Sal del Rey ("the King's Salt") is one of several naturally-occurring salt lakes in Hidalgo County, Texas and is part of the Lower Rio Grande Valley National Wildlife Refuge. The research objective was to isolate and characterize halophilic microorganisms from La Sal del Rey. Water samples were collected from the lake and a small creek that feeds into the lake. Soil samples were collected from land adjacent to the water sample locations. Sample salinity was determined using a refractometer. Samples were diluted and cultured on a synthetic saline medium to grow halophilic bacteria. The density of halophiles was …

Selective Capture Of Transcribed Sequences: A Promising Approach For Investigating Bacterium-Insect Interactions, Ruisheng An, Parwinder Grewal

School of Earth, Environmental, and Marine Sciences Faculty Publications and Presentations

Bacterial interactions with eukaryotic hosts are complex processes which vary from pathogenic to mutualistic. Identification of bacterial genes differentially expressed in the host, promises to unravel molecular mechanisms driving and maintaining such interactions. Several techniques have been developed in the past 20 years to investigate bacterial gene expression within their hosts. The most commonly used techniques include in-vivo expression technology, signature-tagged mutagenesis, differential fluorescence induction, and cDNA microarrays. However, the limitations of these techniques in analyzing bacterial in-vivo gene expression indicate the need to develop alternative tools. With many advantages over the other methods for analyzing bacterial in-vivo gene expression, …