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Full-Text Articles in Physical Sciences and Mathematics
Metal-Resistance Genetically Engineered Bacteria, Sylvia Daunert, Donna Scott, Sridhar Ramanathan
Metal-Resistance Genetically Engineered Bacteria, Sylvia Daunert, Donna Scott, Sridhar Ramanathan
KWRRI Research Reports
Bacterial-based electrochemical and optical sensing systems that respond in a highly selective and sensitive manner to antimonite and arsenite have been developed. This was accomplished by using genetically engineered bacteria bearing one of two plasmids constructed for our studies. The first plasmid, pBGD23, contains the operator/promoter region (O/P) and the gene of the ArsR protein from the ars operon upstream from the β-galactosidase gene. In the absence of antimonite/arsenite, ArsR binds to the 0/P site and prevents the transcription of the genes for ArsR and β-galactosidase, thus blocking expression of these proteins. When antimonite or arsenite is present in the …
Fish Lateral Line Neurophysiological And Neurobehavioral Responses As A Sensitive Water Quality Monitoring System, Hong Y. Yan
Fish Lateral Line Neurophysiological And Neurobehavioral Responses As A Sensitive Water Quality Monitoring System, Hong Y. Yan
KWRRI Research Reports
Cadmium is a heavy metal ion that can cause deleterious effects on aquatic animals. This study uses both electrophysiological recordings from lateral line nerves and videotaping of schooling behavior to investigate the effects of cadmium exposure on fish. The fathead minnows were exposed to cadmium at a concentration of 450 μg/1 over a 24-hr period. Extracellular recording with a silver hook electrode was used to record compound action potentials from the lateral lines of control and experimental fish. After a short time exposure (24 hr) to cadmium ions, all of the electrophysiological activities of the lateral line nerves were suppressed. …