Physical Sciences and Mathematics Commons^™

Developing White Ash With Resistance To The Emerald Ash Borer, Kaitlin Joy Palla

Open Access Theses

Hypocotyls isolated from mature embryos germinated on Murashige and Skoog (MS) medium supplemented with 22.2 µM 6-benzyladenine (BA) and 0.5 µM thidiazuron (TDZ) were transformed using Agrobacterium tumefaciens strain EHA105 harboring the pBI121-8D2 vector containing the neomycin phosphotransferase (nptII) gene and a full length, codon-optimized Bacillus thuringiensis Cry8Da protoxin gene (cry8D2). Explants were transformed in a bacterial suspension with 100 µM acetosyringone using 90 s sonication and 10 min vacuum-infiltration. Four independent lines of transformed shoots were selectively regenerated on MS medium with 22.2 µM BA, 0.5 µM TDZ, 50 mg L^-1 adenine sulfate, 10% …

Specific Salt Effects On The Formation And Thermal Transitions Among Β-Lactoglobulin And Pectin Electrostatic Complexes, Stacey Ann Hirt

Open Access Theses

Factors of ion specificity and ionic strength (I~0-100) were studied in the electrostatic complex formation and protein particle formation by thermal treatment for a β-lactoglobulin and pectin system. ζ-potential showed β-lactoglobulin and pectin began to interact near pH 5.50 and interactions were strengthened with decrease in pH. Visible light turbidimetry and light scattering at 90° revealed a trend in critical pH transitions for electrostatic complex formation based on both the ionic strength and the anion of the salt species, while effects of the monovalent cation was insignificant. Critical pH values for complex formation and separation (pHc and pHΦ) decreased with …

Habitat Restoration Of A Constructed Ohio River Embayment, Caleb Michael Rennaker

Open Access Theses

Backwater habitats of large rivers provide habitat for aquatic biota and influence important predator-prey interactions in fishes and other aquatic organisms. However, these areas often exhibit turbid conditions and lack habitat complexity. Restoration of lentic habitats through direct habitat manipulations has shown success in prior efforts, but these methods have not been tested in backwater habitats of large rivers. I attempted to create habitat in an Ohio River embayment by establishing founder colonies of aquatic macrophytes coupled with the placement of underwater gravel beds. I also evaluated the effects of total suspended solids (TSS) on overwintering structures of American Pondweed …

Pickering Stabilization Of Oil-Water Interfaces By Heated B-Lactoglobulin/Pectin Particles, Laura Kathryn Zimmerer

Open Access Theses

The use of natural biopolymer particles as Pickering stabilizers for oil-in-water emulsions was investigated. B-lactoglobulin microgels and B-lactoglobulin/pectin complexes were created by heating appropriate biopolymer solutions at pH 5.8 and 4.75, respectively. Resultant particles exhibited spherical morphology with diameters of 100-300 nm and possessed significant negative surface charge. Particles were first homogenized with 1% corn oil at 0.05%, 0.1% or 0.25% (wt/ wt) particle concentrations. All emulsions appeared stable over seven days with only a thin, creamed ring forming after several hours. Corn oil emulsions were most stable with 0.25% heated complexes, with volume-weighted mean droplet diameter remaining around 480 …

Improving Model Performance For Invasive Plant Species Distribution Using Global-Scale Presence-Only Data: Parameterization And Data Quality, Feng Yu

Open Access Theses

Invasive species have significant ecological and economic impacts. To control species' invasion, risk assessment provides the most essential information for identification and evaluation of the potential risk of the invasive species, especially in their early invasion stages. Species distribution models (SDMs) is the foundation for risk assessment, in terms of both the practical and theoretical interest in our understanding of species invasion process. SDMs contribute to the proactive invasion management and the test of ecological or biogeographical hypotheses about species distributions in relation to their environment.

However, modeling of invasive species at large spatial scale (i.e., cross-continental) is rarely discussed. …

Swat Model Simulation Of Bioenergy Crop Impacts In A Tile-Drained Watershed, Chelsie Marie Werling Boles

Open Access Theses

Tile drains are an important component of agricultural production in the Midwest, and their inclusion in modeling studies is important in watersheds where they are a principal hydrologic pathway. The new tile drainage simulation method in the Soil Water Assessment Tool (SWAT) was parameterized and tile flow results were compared with reviewed literature. Streamflow, sediment, and nutrient outputs were compared to measured values and simulated crop yields were examined with respect to average county yields. Plant growth stressors were examined to account for differences between simulated and published yields. The bioenergy crop switchgrass (Panicum virgatum) was applied over …

Slurry Seeding Cover Crops, Edwin Alexander Suarez

Open Access Theses

Cover crops improve soil quality through increased organic matter, biological activity, aggregate stability, water infiltration, and nutrient cycling. Slurry seeding, the co-application of cover crop seed and swine (Sus domesticus) manure, is a new practice that saves fuel, time and labor. High salinity and/or ammonia, among other components in swine manure, may reduce cover crop seed germination and limit the use of slurry seeding. Ten cover crop species were evaluated to compare the effects of swine manure components on cover crop seed germination. Seed germination percentages and rates were affected by swine manure but not by equivalent salinity levels established …

A Spatially Explicit Watershed Scale Optimization Of Cellulosic Biofuels Production, Jingyu Song

Open Access Theses

Producing biofuels from energy crops is one such alternative. They can result in relatively lower greenhouse gas emissions compared to traditional energy sources. Up to now, corn grain is the most researched energy crop. Cellulosic perennial crops such as switchgrass, miscanthus and fast growing trees are also promising energy crops and are expected to help with the energy supply. The 2007 Renewable Fuel Standard requires 16 billion gallons of a total of 36 billion gallons of renewable fuels to be cellulosic biofuels by 2022. Many studies are being done to evaluate costs and feasibility of different potential feedstocks and the …