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Articles 1 - 5 of 5
Full-Text Articles in Physical Sciences and Mathematics
Single-Cell Growth Rates In Photoautotrophic Populations Measured By Stable Isotope Probing And Resonance Raman Microspectrometry, Gordon T. Taylor, Elizabeth A. Suter, Zhuo Q. Li, Stephanie Chow, Dallyce Stinton, Tatiana Zaliznyak, Steven R. Beaupre
Single-Cell Growth Rates In Photoautotrophic Populations Measured By Stable Isotope Probing And Resonance Raman Microspectrometry, Gordon T. Taylor, Elizabeth A. Suter, Zhuo Q. Li, Stephanie Chow, Dallyce Stinton, Tatiana Zaliznyak, Steven R. Beaupre
Faculty Works: Biology, Chemistry, and Environmental Studies
A newmethod tomeasure growth rates of individual photoautotrophic cells by combining stable isotope probing (SIP) and single-cell resonance Raman microspectrometry is introduced. This report explores optimal experimental design and the theoretical underpinnings for quantitative responses of Raman spectra to cellular isotopic composition. Resonance Raman spectra of isogenic cultures of the cyanobacterium, Synechococcus sp., grown in 13C-bicarbonate revealed linear covariance between wavenumber (cm−1) shifts in dominant carotenoid Raman peaks and a broad range of cellular 13C fractional isotopic abundance. Single-cell growth rates were calculated from spectra-derived isotopic content and empirical relationships. Growth rates among any 25 cells in a sample varied …
The Coastal Monitor: Vol 2. No 1., John Tanacredi Ph.D.
The Coastal Monitor: Vol 2. No 1., John Tanacredi Ph.D.
The Coastal Monitor
There is something enchanting and vibrant about viewing the coast. Every day at CERCOM we are welcomed by either a vivid sunrise, a view of the barrier island Fire Island and a blending of the sky across the horizon, ultimately flooding the Great South Bay with either clouds or sunshine that reveals the Bay’s surface as either shimmering with sunlight, or an integration of sky and water as an endless ocean. I don’t normally wax poetic but the varying sights along the near shore ocean and this embayment beckon our scientific inquisitiveness. What’s below the surface water? How do animals …
The Stress Of Public Speaking Increases Cortisol Levels In Undergraduates: Is Increased Preparation Really The Best Remedy?, Jodi F. Evans Ph.D., Erin Clinton, Grace Cookson, Stephanie Brown, Daniel Woods
The Stress Of Public Speaking Increases Cortisol Levels In Undergraduates: Is Increased Preparation Really The Best Remedy?, Jodi F. Evans Ph.D., Erin Clinton, Grace Cookson, Stephanie Brown, Daniel Woods
Faculty Works: Biology, Chemistry, and Environmental Studies
Perceived stress is prevalent among the undergraduate population. When this stress persists, it has the potential to lead to mental health illnesses. Recent research shows 85% of students experience overwhelming anxiety from academic pressures. Physiologically, during stressful events, cortisol levels rise in the body which disrupts homeostasis. The anticipation prior to a class presentation, a form of public speaking, is a common source of perceived stress among undergraduates. The focus of this experiment was to determine if there is a correlation between factors such as increased preparedness, sleep, level of understanding, perceived anxiety and physiological stress parameters. Twenty-eight student volunteers …
Cd105 Deficieny In Mouse Aorta-Derived Mesenchymal Stem Cells Promotes An Enhanced Inflammatory Response To Lipolysaccharide., Jodi F. Evans Ph.D., Joseph Granata, Hugo Sanchez, Philip Loeschinger, Anthony Goez
Cd105 Deficieny In Mouse Aorta-Derived Mesenchymal Stem Cells Promotes An Enhanced Inflammatory Response To Lipolysaccharide., Jodi F. Evans Ph.D., Joseph Granata, Hugo Sanchez, Philip Loeschinger, Anthony Goez
Faculty Works: Biology, Chemistry, and Environmental Studies
Mesenchymal stem cells (MSCs) are being widely studied for their ability to regulate macrophage cell responses. Previous works have demonstrated that mouse aorta-derived MSC (mAo-MSC) support the macrophage inflammatory response. mAo-MSC have been characterized phenotypically for MSC-associated surface antigens and express CD90 and CD105 but do not express CD73. CD105, also known as endoglin, is a coreceptor in the TGFβ superfamily of receptors. Mouse adipose-derived MSC lacking CD105 have an increased capacity to regulate T-cells by reducing their proliferation while elevated CD105 expression is consistently associated with inflammatory disease. Therefore, we hypothesized that suppression of CD105 in mAo-MSC will reduce …
Mouse Mesenchymal Progenitor Cells Expressing Adipogenic And Osteogenic Transcription Factors Suppress The Macrophage Inflammatory Response, Jodi F. Evans Ph.D., Natalie Fernandez, Heather Renna, Lauren Mchugh, Katie Mazolkova, William Crugnola
Mouse Mesenchymal Progenitor Cells Expressing Adipogenic And Osteogenic Transcription Factors Suppress The Macrophage Inflammatory Response, Jodi F. Evans Ph.D., Natalie Fernandez, Heather Renna, Lauren Mchugh, Katie Mazolkova, William Crugnola
Faculty Works: Biology, Chemistry, and Environmental Studies
Mesenchymal progenitor cell characteristics that can identify progenitor populations with specific functions in immunity are actively being investigated. Progenitors from bone marrow and adipose tissue regulate the macrophage (MΦ) inflammatory response by promoting the switch froman inflammatory to an anti-inflammatory phenotype.Conversely,mesenchymal progenitors fromthe mouse aorta (mAo) support and contribute to the MΦ response under inflammatory conditions.We used cell lines with purported opposing immune-regulatory function, a bonemarrow derivedmesenchymal progenitor cell line (D1) and amouse aorta derived mesenchymal progenitor cell line (mAo). Their interaction and regulation of the MΦ cell response to the inflammatory mediator, lipopolysaccharide (LPS), was examined by coculture. As …