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Mobility Of Escherichia Coli Within Karst Terrains, Kentucky, Usa, Ashley M. Bandy
Mobility Of Escherichia Coli Within Karst Terrains, Kentucky, Usa, Ashley M. Bandy
Theses and Dissertations--Earth and Environmental Sciences
Bacterial contamination of karst aquifers is a concern as water quality across the globe deteriorates in the face of decreasing water security. This study examined the transport and attenuation of two non-virulent isolates of Escherichia coli in relation to traditional groundwater tracers such as rhodamine WT dye and latex microspheres in two karst regions in Kentucky. Differential movement between the four tracers was observed in both epikarst and karst aquifer traces, with differences in behavior dependent on flow conditions. Attenuation was greater for the bacterial isolate containing the iha gene, compared to the isolate containing the kps gene. Microspheres of …
Fate Of Stable Isotope Label During Predation Of 15N-Tagged Wild-Type Escherichia Coli By Protozoa, Ashley M. Barton
Fate Of Stable Isotope Label During Predation Of 15N-Tagged Wild-Type Escherichia Coli By Protozoa, Ashley M. Barton
University of Kentucky Master's Theses
Currently, bacterial movement in karst aquifers is not well understood. Use of stable isotopes to label non-pathogenic Escherichia coli as a particulate groundwater tracer in karst systems has been examined in previous studies. Loss of the stable isotope signal is anticipated in traces greater than 500 m in length. Potential loss of 15N due to predation by protozoa was examined. Filter-sterilized water from Royal Spring in Georgetown, Kentucky, was inoculated with a mixture of either Tetrahymena pyriformis or Colpoda steinii and 15N-enriched E. coli and stored in the dark at 14°C. Samples were analyzed for their nitrogen isotope …