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Articles 1 - 7 of 7
Full-Text Articles in Physical Sciences and Mathematics
Microbial Nanowires: Is The Subsurface "Hardwired"?, Dimitrios Ntarlagiannis, Estella A. Atekwana, Eric A. Hill, Yuri A. Gorby
Microbial Nanowires: Is The Subsurface "Hardwired"?, Dimitrios Ntarlagiannis, Estella A. Atekwana, Eric A. Hill, Yuri A. Gorby
Geosciences and Geological and Petroleum Engineering Faculty Research & Creative Works
The Earth's shallow subsurface results from integrated biological, geochemical, and physical processes. Methods are sought to remotely assess these interactive processes, especially those catalysed by micro-organisms. Using saturated sand columns and the metal reducing bacterium Shewanella oneidensis MR-1, we show that electrically conductive appendages called bacterial nanowires are directly associated with electrical potentials. No significant electrical potentials were detectable in columns inoculated with mutant strains that produced non-conductive appendages. Scanning electron microscopy imaging revealed a network of nanowires linking cells-cells and cells to mineral surfaces, "hardwiring" the entire length of the column. We hypothesize that the nanowires serve as conduits …
Induced-Polarization Measurements On Unconsolidated Sediments From A Site Of Active Hydrocarbon Biodegradation, Gamal Z. Abdel Aal, Lee D. Slater, Estella A. Atekwana
Induced-Polarization Measurements On Unconsolidated Sediments From A Site Of Active Hydrocarbon Biodegradation, Gamal Z. Abdel Aal, Lee D. Slater, Estella A. Atekwana
Geosciences and Geological and Petroleum Engineering Faculty Research & Creative Works
To investigate the potential role that indigenous microorganisms and microbial processes may play in altering low frequency electrical properties, induced-polarization (IP) measurements in the frequency range of 0.1 to 1000 Hz were acquired from sediment samples retrieved from a site contaminated by hydrocarbon undergoing intrinsic biodegradation. Increased imaginary conductivity and phase were observed for samples from the smear zone (contaminated with residual-phase hydrocarbon), exceeding values obtained for samples contaminated with dissolved-phase hydrocarbons, and in turn, exceeding values obtained for uncontaminated samples. Real conductivity, although generally elevated for samples from the smear zone, did not show a strong correlation with contamination. …
Evidence For Microbial Enhanced Electrical Conductivity In Hydrocarbon-Contaminated Sediments, Estella A. Atekwana, Eliot A. Atekwana, D. Dale Werkema, Jonathan P. Allen, Laura A. Smart, Joseph W. Duris, Daniel P. Cassidy, William A. Sauck, Silvia Rossbach
Evidence For Microbial Enhanced Electrical Conductivity In Hydrocarbon-Contaminated Sediments, Estella A. Atekwana, Eliot A. Atekwana, D. Dale Werkema, Jonathan P. Allen, Laura A. Smart, Joseph W. Duris, Daniel P. Cassidy, William A. Sauck, Silvia Rossbach
Geosciences and Geological and Petroleum Engineering Faculty Research & Creative Works
Bulk electrical conductivity of sediments during microbial mineralization of diesel was investigated in a mesoscale laboratory experiment consisting of biotic contaminated and uncontaminated columns. Population numbers of oil degrading microorganisms increased with a clear pattern of depth zonation within the contaminated column not observed in the uncontaminated column. Microbial community structure determined from ribosomal DNA intergenic spacer analysis showed a highly specialized microbial community in the contaminated column. The contaminated column showed temporal increases in bulk conductivity, dissolved inorganic carbon, and calcium, suggesting that the high bulk conductivity is due to enhanced mineral weathering from microbial activity. The greatest change …
Field Evidence For Geophysical Detection Of Subsurface Zones Of Enhanced Microbial Activity, Eliot A. Atekwana, Estella A. Atekwana, Franklyn D. Legall, R. V. Krishnamurthy
Field Evidence For Geophysical Detection Of Subsurface Zones Of Enhanced Microbial Activity, Eliot A. Atekwana, Estella A. Atekwana, Franklyn D. Legall, R. V. Krishnamurthy
Geosciences and Geological and Petroleum Engineering Faculty Research & Creative Works
Geochemical data from closely spaced vertical intervals in a hydrocarbon-impacted aquifer were used to assess the relationship between bulk conductivity and zones of enhanced microbial activity. The bulk conductivity was measured using in situ vertical resistivity probes. Microbial activity was verified using terminal electron acceptors (nitrate, sulfate, iron, and manganese), dissolved inorganic carbon (DIC), and major ion chemistry. Peaks in bulk conductivity in the aquifer overlapped with zones where nitrates and sulfates were depleted, total petroleum hydrocarbon, iron, manganese, dissolved ions, and DIC were elevated, suggesting a link between higher electrical conductivity and zones of enhanced microbial activity stimulated by …
Effects Of Microbial Processes On Electrolytic And Interfacial Electrical Properties Of Unconsolidated Sediments, Gamal Z. Abdal Aal, Estella A. Atekwana, Lee D. Slater, Eliot A. Atekwana
Effects Of Microbial Processes On Electrolytic And Interfacial Electrical Properties Of Unconsolidated Sediments, Gamal Z. Abdal Aal, Estella A. Atekwana, Lee D. Slater, Eliot A. Atekwana
Geosciences and Geological and Petroleum Engineering Faculty Research & Creative Works
The effect of microbial processes on electrical properties of unconsolidated sediments was investigated in a laboratory experiment consisting of biotic and abiotic sand columns. The biotic column (nutrient, diesel and bacteria) showed (a) temporal increase in the real, imaginary, and surface conductivity, and (b) temporal decrease in the formation factor. The abiotic columns (nutrient; and nutrient and diesel) showed no significant changes. Increase in microbial population numbers, decrease in organic carbon source, nitrate, and sulfate and increase in dissolved inorganic carbon and fluid conductivity were indicative of microbial activity in the biotic column. We also measure relative increase in the …
In-Situ Apparent Conductivity Measurements And Microbial Population Distribution At A Hydrocarbon-Contaminated Site, Estella A. Atekwana, D. Dale Werkema, Joseph W. Duris, Silvia Rossbach, Eliot A. Atekwana, William A. Sauck, Daniel P. Cassidy, Jay Means, Franklyn D. Legall
In-Situ Apparent Conductivity Measurements And Microbial Population Distribution At A Hydrocarbon-Contaminated Site, Estella A. Atekwana, D. Dale Werkema, Joseph W. Duris, Silvia Rossbach, Eliot A. Atekwana, William A. Sauck, Daniel P. Cassidy, Jay Means, Franklyn D. Legall
Geosciences and Geological and Petroleum Engineering Faculty Research & Creative Works
We investigated the bulk electrical conductivity and microbial population distribution in sediments at a site contaminated with light nonaqueous-phase liquid (LNAPL). The bulk conductivity was measured using in-situ vertical resistivity probes; the most probable number method was used to characterize the spatial distribution of aerobic heterotrophic and oil-degrading microbial populations. The purpose of this study was to assess if high conductivity observed at aged LNAPL-impacted sites may be related to microbial degradation of LNAPL. The results show higher bulk conductivity coincident with LNAPL-impacted zones, in contrast to geoelectrical models that predict lower conductivity in such zones. The highest bulk conductivity …
Investigating The Geoelectrical Response Of Hydrocarbon Contamination Undergoing Biodegradation, D. Dale Werkema, Estella A. Atekwana, Anthony L. Endres, William August Sauck, Daniel P. Cassidy
Investigating The Geoelectrical Response Of Hydrocarbon Contamination Undergoing Biodegradation, D. Dale Werkema, Estella A. Atekwana, Anthony L. Endres, William August Sauck, Daniel P. Cassidy
Geosciences and Geological and Petroleum Engineering Faculty Research & Creative Works
A newly proposed geoelectrical model for hydrocarbon contaminated sites predicts high conductivities coincident with the contaminated zone as opposed to the traditionally accepted low conductivity. The model attributes the high conductivities to mineral weathering resulting from byproducts of microbial redox processes. To evaluate this conductive model, in situ vertical conductivity measurements were acquired from a light non-aqueous phase liquid (LNAPL) contaminated site. The results showed high conductivities coincident with the zone of contamination and within the smear zone influenced by seasonal water table fluctuations. We infer this zone as an active zone of biodegradation and suggest significant microbial degradation under …