Physical Sciences and Mathematics Commons^™

Performance Of Human Fecal Anaerobe-Associated Pcr-Based Assays In A Multi-Laboratory Method Evaluation Study, Blythe A. Layton, Yiping Cao, Darcy L. Ebentier, Kaitlyn Hanley, Elisenda Balleste, Joao Brandao, Muruleedhara Byappanahalli, Reagan Converse, Andreas H. Farnleitner, Jennifer Gentry-Shields, Maribeth L. Gidley, Michele Gourmelon, Chang Soo Lee, Jiyoung Lee, Solen Lozach, Tania Madi, Wim G. Meijer, Rachel Noble, Lindsay Peed, Georg H. Reischer, Raquel Rodrigues, Joan B. Rose, Alexander Schriewer, Chris Sinigalliano, Sangeetha Srinivasan, Jill Stewart, Laurie C. Van De Werfhorst, Dan Wang, Richard Whitman, Stefan Wuertz, Jenny Jay, Patricia A. Holden, Alexandria B. Boehm, Orin Shanks, John F. Griffith

United States Geological Survey: Staff Publications

A number of PCR-based methods for detecting human fecal material in environmental waters have been developed over the past decade, but these methods have rarely received independent comparative testing in large multi-laboratory studies. Here, we evaluated ten of these methods (BacH, BacHum-UCD, Bacteroides thetaiotaomicron (BtH), BsteriF1, gyrB, HF183 endpoint, HF183 SYBR, HF183 Taqman^®, HumM2, and Methanobrevibacter smithii nifH (Mnif)) using 64 blind samples prepared in one laboratory. The blind samples contained either one or two fecal sources from human, wastewater or non-human sources. The assay results were assessed for presence/absence of the human markers and also quantitatively while …