Physical Sciences and Mathematics Commons^™

Microbial Degradation Of Fuel Oxygenates Under Aerobic Conditions, John M. Dietz

Theses and Dissertations

This research determined the rate and extent of aerobic biodegradation of fuel oxygenates ethyl tert butyl ether (ETBE), tert amyl methyl ether (TAME), and ethyl alcohol (ethanol). Biodegradation was measured using gas chromatography (GC), respirometry, and biochemical oxygen demand (BOD) tests. Additionally, the research determined the effects of toluene on degradation rates. This microcosm study used a microbial consortium obtained from a petroleum refinery wastewater treatment facility. Respirometry data were collected from chambers containing pure oxygenates, or oxygenate/toluene mixtures. Samples were withdrawn periodically for GC analysis. Aerobic conditions were maintained in the chambers at all times. The five-day BOD test …