Physical Sciences and Mathematics Commons^™

Dgge Analysis Of Denitrifying Bacterial Diversity In Response To Nutrients And Oil Contamination In Salt Marshes, Catalina Restrepo

LSU Master's Theses

Salt marsh ecosystems located at the Mississippi River delta are exposed to high levels of point and nonpoint sources of nutrients such as nitrate-based fertilizers and to oil spills in the Gulf of Mexico. However, there is little information on the effects of eutrophication and oil contamination on the bacterial diversity of denitrifying bacteria in salt marsh sediments of coastal Louisiana. The effects of excess nutrients on the bacterial diversity of denitrifiers were analyzed by injecting sediment cores with high concentrations of nitrate (100μM NO3- and 20,000μM NO3-) and carbon (0% and 20% C concentration from the original sample). After …