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Full-Text Articles in Physical Sciences and Mathematics

Comparison Of Protein Phosphatase Inhibition Assay With Lc-Ms/Ms For Diagnosis Of Microcystin Toxicosis In Veterinary Cases, Caroline E. Moore, Jeanette Juan, Yanping Lin, Cynthia L. Gaskill, Birgit Puschner Mar 2016

Comparison Of Protein Phosphatase Inhibition Assay With Lc-Ms/Ms For Diagnosis Of Microcystin Toxicosis In Veterinary Cases, Caroline E. Moore, Jeanette Juan, Yanping Lin, Cynthia L. Gaskill, Birgit Puschner

Veterinary Diagnostic Laboratory Faculty Publications

Microcystins are acute hepatotoxins of increasing global concern in drinking and recreational waters and are a major health risk to humans and animals. Produced by cyanobacteria, microcystins inhibit serine/threonine protein phosphatase 1 (PP1). A cost-effective PP1 assay using p-nitrophenyl phosphate was developed to quickly assess water and rumen content samples. Significant inhibition was determined via a linear model, which compared increasing volumes of sample to the log-transformed ratio of the exposed rate over the control rate of PP1 activity. To test the usefulness of this model in diagnostic case investigations, samples from two veterinary cases were tested. In August …


Effects Of Mountaintop Removal Mining On Population Dynamics Of Stream Salamanders, Sara B. Freytag Jan 2016

Effects Of Mountaintop Removal Mining On Population Dynamics Of Stream Salamanders, Sara B. Freytag

Theses and Dissertations--Forestry and Natural Resources

Mountaintop removal mining (MTR) is a notorious stressor of stream ecosystems in the Central Appalachians. Valley fills (VF) lead to reduced occupancy, abundance, and species richness of stream salamanders. Multiple factors may be responsible for these reductions, but specifically habitat fragmentation and degradation may reduce colonization rates and increase local extinction rates. From 2013-2015, repeated counts of salamanders were conducted in stream reaches impacted by MTR/VF and compared to counts in reference reaches to answer the question: do stream salamander population dynamics differ between stream reaches impacted by MTR/VF and reference stream reaches? I also investigated dynamics of stream habitat …


Heavy-Oil And Bitumen Resources Of The Big Clifty Sandstone, Northeastern Grayson County And Adjacent Hardin County, Kentucky, J. Richard Bowersox Jan 2016

Heavy-Oil And Bitumen Resources Of The Big Clifty Sandstone, Northeastern Grayson County And Adjacent Hardin County, Kentucky, J. Richard Bowersox

Report of Investigations--KGS

Rock asphalt (bitumen-saturated sandstone) was produced from the Big Clifty Sandstone near Tar Hill and Big Clifty in northeastern Grayson County, and at Summit in adjacent Hardin County, from 1889 to 1940. Noncommercial amounts of oil were distilled from Big Clifty rock asphalt before 1930. Resource assessments conducted throughout the area during the mid-1920's described substantial rock-asphalt deposits. Later assessments in 1951, 1965, and the early 1980's, however, overlooked the northeastern Grayson County area. A new evaluation in 2015 estimated that the historically developed area between Clifty Creek and meeting Creek, and between the Summit Fault and Eveleigh Fault Zone, …