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Enhanced Biotransformation Of Carbon Tetrachloride By Acetobacterium Woodii Upon Addition Of Hydroxocobalamin And Fructose, Syed Hashsham, David Freedman
Enhanced Biotransformation Of Carbon Tetrachloride By Acetobacterium Woodii Upon Addition Of Hydroxocobalamin And Fructose, Syed Hashsham, David Freedman
David L. Freedman
The objective of this study was to evaluate the effect of hydroxocobalamin (OH-Cbl) on transformation of high concentrations of carbon tetrachloride (CT) by Acetobacterium woodii (ATCC 29683). Complete transfor-mation of 470 M (72 mg/liter [aqueous]) CT was achieved by A. woodii within 2.5 days, when 10 M OH-Cbl was added along with 25.2 mM fructose. This was approximately 30 times faster than A. woodii cultures (live or autoclaved) and medium that did not receive OH-Cbl and 5 times faster than those controls that did receive OH-Cbl, but either live A. woodii or fructose was missing. CT transformation in treatments with …