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Articles 1 - 19 of 19
Full-Text Articles in Physical Sciences and Mathematics
Approaches To Studying Bacterial Biofilms In The Bioeconomy With Nanofabrication Techniques And Engineered Platforms., Michelle Caroline Halsted
Approaches To Studying Bacterial Biofilms In The Bioeconomy With Nanofabrication Techniques And Engineered Platforms., Michelle Caroline Halsted
Doctoral Dissertations
Studies that estimate more than 90% of bacteria subsist in a biofilm state to survive environmental stressors. These biofilms persist on man-made and natural surfaces, and examples of the rich biofilm diversity extends from the roots of bioenergy crops to electroactive biofilms in bioelectrochemical reactors. Efforts to optimize microbial systems in the bioeconomy will benefit from an improved fundamental understanding of bacterial biofilms. An understanding of these microbial systems shows promise to increase crop yields with precision agriculture (e.g. biosynthetic fertilizer, microbial pesticides, and soil remediation) and increase commodity production yields in bioreactors. Yet conventional laboratory methods investigate these micron-scale …
Carbon Metabolism In Cave Subaerial Biofilms, Victoria E. Frazier
Carbon Metabolism In Cave Subaerial Biofilms, Victoria E. Frazier
Masters Theses
Subaerial biofilms (SABs) grow at the interface between the atmosphere and rock surfaces in terrestrial and subterranean environments around the world. Multi-colored SABs colonizing relatively dry and nutrient-limited cave surfaces are known to contain microbes putatively involved in chemolithoautotrophic processes using inorganic carbon like carbon dioxide (CO2) or methane (CH4). However, the importance of CO2 and CH4 to SAB biomass production has not been quantified, the environmental conditions influencing biomass production and diversity have not been thoroughly evaluated, and stable carbon and nitrogen isotope compositions have yet to be determined from epigenic cave SABs. …
Production And Characterization Of Twelve Different Biochars And Evaluating Their Effects On Soil Health And Plant Growth, Shagufta Gaffar
Production And Characterization Of Twelve Different Biochars And Evaluating Their Effects On Soil Health And Plant Growth, Shagufta Gaffar
FIU Electronic Theses and Dissertations
Biochar has been a topic of growing interest in the scientific community. It is a product derived from carbon rich organic materials through the process of pyrolysis. It has received wide attention as a means to improve soil fertility and crop productivity, absorb pollutants in soil, and sequester carbon to mitigate climate change. Recent research on biochar explores its impacts on the environment with particular focus on use as a soil amendment in agriculture. Biochar produced from different biomass and under different production process effects the environmental and agronomic impacts of its application in different ways. This means biochar can …
Aspergillus Niger Decreases Bioavailability Of Arsenic(V) Via Biotransformation Of Manganese Oxide Into Biogenic Oxalate Minerals, Bence Farkas, Marek Kolenčík, Miroslav Hain, Edmund Dobročka, Gabriela Kratošová, Marek Bujdoš, Huan Feng, Yang Deng, Qian Yu, Ramakanth Illa, B. Ratna Sunil, Hyunjung Kim, Peter Matúš, Martin Urík
Aspergillus Niger Decreases Bioavailability Of Arsenic(V) Via Biotransformation Of Manganese Oxide Into Biogenic Oxalate Minerals, Bence Farkas, Marek Kolenčík, Miroslav Hain, Edmund Dobročka, Gabriela Kratošová, Marek Bujdoš, Huan Feng, Yang Deng, Qian Yu, Ramakanth Illa, B. Ratna Sunil, Hyunjung Kim, Peter Matúš, Martin Urík
Department of Earth and Environmental Studies Faculty Scholarship and Creative Works
The aim of this work was to evaluate the transformation of manganese oxide (hausmannite) by microscopic filamentous fungus Aspergillus niger and the effects of the transformation on mobility and bioavailability of arsenic. Our results showed that the A. niger strain CBS 140837 greatly affected the stability of hausmannite and induced its transformation into biogenic crystals of manganese oxalates—falottaite and lindbergite. The transformation was enabled by fungal acidolysis of hausmannite and subsequent release of manganese ions into the culture medium. While almost 45% of manganese was bioextracted, the arsenic content in manganese precipitates increased throughout the 25-day static cultivation of fungus. …
Trace Metal Availability Affects Greenhouse Gas Emissions And Microbial Functional Group Abundance In Freshwater Wetland Sediments, George Giannopoulos, Katherine R. Hartop, Bonnie L. Brown, Bongkeun Song, Lars Elsgaard, Rima B. Franklin
Trace Metal Availability Affects Greenhouse Gas Emissions And Microbial Functional Group Abundance In Freshwater Wetland Sediments, George Giannopoulos, Katherine R. Hartop, Bonnie L. Brown, Bongkeun Song, Lars Elsgaard, Rima B. Franklin
VIMS Articles
We investigated the effects of trace metal additions on microbial nitrogen (N) and carbon (C) cycling using freshwater wetland sediment microcosms amended with micromolar concentrations of copper (Cu), molybdenum (Mo), iron (Fe), and all combinations thereof. In addition to monitoring inorganic N transformations (NO3–, NO2–, N2O, NH4+) and carbon mineralization (CO2, CH4), we tracked changes in functional gene abundance associated with denitrification (nirS, nirK, nosZ), dissimilatory nitrate reduction to ammonium (DNRA; nrfA), and methanogenesis (mcrA). With regards to …
Using Soil Geospatial Properties And Environments To Explore Microbial Diversity, Sharon Faye Smith
Using Soil Geospatial Properties And Environments To Explore Microbial Diversity, Sharon Faye Smith
Graduate Theses and Dissertations
Soil microorganisms help maintain nutrient cycling, control carbon sequestration, impact plant productivity, and influence several soil chemical and physical properties; yet, the processes that control the microbial composition of soil and how environmental changes may affect the composition and activity of these organisms at different scales remains a difficult and intriguing puzzle for soil scientists, ecologists, and modelers. Wetlands are endangered and important ecosystems that provide several services, which are directly linked to soil function. However, few wetland assessments consider the soil environment and microbial ecology. Linking soil microbial community composition and distribution patterns to soil physio-chemical properties would provide …
Analysis Of Biofilm Remediation Capacity For Octenyl Succinic Anhydride (Osa), A Bioactive Food Starch Modifier Compound, Matthew R. Borglin
Analysis Of Biofilm Remediation Capacity For Octenyl Succinic Anhydride (Osa), A Bioactive Food Starch Modifier Compound, Matthew R. Borglin
Master's Theses
Matthew R. Borglin
This thesis demonstrates efficacy of Octenyl Succinic Anhydride (OSA), as a biofilm sanitizer. Biofilms allow bacteria to adhere to solid surfaces with the use of excreted polymeric compounds. For example, surfaces found in food production or processing facilities such as the interior of a raw milk holding tank, are some of the most susceptible to biofilm contamination. When present, biofilms can cause a variety of negative effects, which include; reduction of product shelf life, corrosion, and outbreaks of foodborne illnesses. The close association of biofilms with the majority of foodborne illness cases led the US Environmental Protection …
Mathematical Modeling Of Gliding Motility And Its Regulation In Myxococcus Xanthus, Yirui Chen
Mathematical Modeling Of Gliding Motility And Its Regulation In Myxococcus Xanthus, Yirui Chen
Biology and Medicine Through Mathematics Conference
No abstract provided.
Effects Of Warming On Decomposition Of Submerged Plant Litter And Associated Microorganisms In Streamside Channels, Kaity Ackerman
Effects Of Warming On Decomposition Of Submerged Plant Litter And Associated Microorganisms In Streamside Channels, Kaity Ackerman
Electronic Theses and Dissertations
Surface air temperatures are predicted to increase in the near future, which will likely affect microbial activity and carbon flow in stream ecosystems. I performed an experiment in streamside channels at Coweeta Hydrological Laboratory, NC to assess responses of litter-associated microorganisms to moderate increases in water temperature (5 levels, ambient to +4°C). The objectives of the experiment were to determine: (1) if there are differences in the magnitude of responses to temperature among various microbial parameters and (2) whether microbial responses to temperature vary among plant litter of different carbon quality. Thus, I measured litter decomposition rate, fungal biomass (ergosterol), …
Effects Of Light, Nutrients, And Salts On Microbial Biofilm Productivity And Detrital Processing In Aquatic Mesocosms, Bethanie Brooke Howard-Parker
Effects Of Light, Nutrients, And Salts On Microbial Biofilm Productivity And Detrital Processing In Aquatic Mesocosms, Bethanie Brooke Howard-Parker
Graduate Theses and Dissertations
Anthropogenic activities associated with urbanization, agriculture, and resource extraction continue to increase to support increasing needs of the growing population. These activities increase the amounts of pollutants entering freshwater streams and put aquatic ecosystems at structural and functional risk. Aquatic microbes play an important role in detrital processing in streams as a key linkage in moving carbon from detrital stocks into aquatic food webs. My research investigates the effects of light, nutrients, and salts on detrital microbes and decomposition in freshwaters using a mesocosm approach. In chapter one, I modified a current priming effect (PE) hypothesis model to include light …
The Release, Transport, And Utilization Of Phosphorus From Bed-Sediments: A Study Of A Eutrophic Littoral Cove On Beaver Lake In Northwest Arkansas, James A. Mccarty
The Release, Transport, And Utilization Of Phosphorus From Bed-Sediments: A Study Of A Eutrophic Littoral Cove On Beaver Lake In Northwest Arkansas, James A. Mccarty
Graduate Theses and Dissertations
Eutrophication of surface waters not only impacts the environment but also water treatment processes, the most significant of which is from the effects of algae. During peak algal growth in many southern U.S. reservoirs, inflows that bring nutrients are at an annual minimum, and phosphorus released from bed-sediments is trapped in the hypolimnion. Littoral areas, described as the most productive zone of the lake, may be a possible source of phosphorus that fuels algal growth in the reservoir. I studied an isolated shallow cove in the War Eagle Creek arm of Beaver Lake in Northwest Arkansas to measure, quantify, and …
Soil Microbial Diversity And Litter Decomposition Increase With Time Since Land Use Disturbance In Tropical Montane Forests Of Malaysian Borneo, Renee Sniegocki
Soil Microbial Diversity And Litter Decomposition Increase With Time Since Land Use Disturbance In Tropical Montane Forests Of Malaysian Borneo, Renee Sniegocki
Graduate Theses and Dissertations
Logging and forest conversion are occurring at alarming rates in the tropical forests of Southeast Asia. These disturbances alter soil chemistry, microbial diversity, and disrupt carbon cycling through shifts in litter decomposition. Direct links between microbial diversity and soil properties such as pH are well established; however, the indirect impacts of logging and forest conversion on microbial diversity and litter decomposition are poorly understood. We used surface (5 cm) soil to assess soil physicochemical properties, next-generation DNA sequencing to assess soil microbial diversity, and standardized litterbags to assess litter decomposition stabilization at five sites along a land use disturbance gradient …
Contraction Analysis Of Functional Competitive Lotka-Volterra Systems: Understanding Competition Between Modified Bacteria And Plasmodium Within Mosquitoes., Nickolas Goncharenko
Contraction Analysis Of Functional Competitive Lotka-Volterra Systems: Understanding Competition Between Modified Bacteria And Plasmodium Within Mosquitoes., Nickolas Goncharenko
Electronic Thesis and Dissertation Repository
We propose and analyze an extension to the classic Competitive Lotka-Volterra (CLV) model. The goal is to model competition between species, with a response from the environment. This response is a function of the population of all species and can represent numerous physical phenomena including resource limitation and immune response of a host due to infection. We name this new system a Functional Competitive Lotka-Volterra (FCLV) model. We mainly use the construction of contraction metrics, to determine global properties of the model. We use this result to analyze the competition between Plasmodium sp. and genetically engineered bacteria within the midgut …
Genetic And Biochemical Characterization Of Ergot Alkaloid Synthesizing Fungi And Their Symbionts, Matthew Duane Maust
Genetic And Biochemical Characterization Of Ergot Alkaloid Synthesizing Fungi And Their Symbionts, Matthew Duane Maust
Graduate Theses, Dissertations, and Problem Reports
Ergot alkaloids are fungal tryptophan derived toxins which affect mammalian circulation and neurotransmission. These compounds are biosynthesized by a conserved genetic pathway, known as the ergot alkaloid synthesis (EAS) pathway by fungi belonging to the ascomycete families Trichocomaceae and Clavicipitaceae. Several Ipomoea species and related plants in the morning glory family harbor vertically transmitted symbiotic fungi in the genus Periglandula, also members of Clavicipitaceae, that produce ergot alkaloids. Metabolomic analysis of seeds identified a previously uncharacterized glycoside form of the pharmaceutically important ergot alkaloid, ergonovine. Several species belonging to the fungal genus Metarhizium have recently been shown to have the …
Microbial Responses To Unconventional Oil And Gas Development May Alter Ecosystem Function In Headwater Streams, Rachel Michaels
Microbial Responses To Unconventional Oil And Gas Development May Alter Ecosystem Function In Headwater Streams, Rachel Michaels
Graduate Theses, Dissertations, and Problem Reports
The demand for natural gas, and the need for efficient extraction, has led to the development of unconventional oil and natural gas (UOG) techniques. Due to the novelty of UOG, the potential impacts to freshwater ecosystems are not fully understood. We used a dual pronged approach to study the effects of UOG development on microbial biodiversity and function via a laboratory microcosm experiment and a survey study of streams with and without UOG development within their watersheds. The laboratory microcosm study simulated stream contamination with produced water, a byproduct of UOG operation, using sediment collected from one high water-quality stream …
Investigating Links Between Soil Microbial Structure And Function In Three Major Plant Communities Across Temporal Scales Of Arctic Alaska, Kaj Lynoe
Electronic Theses and Dissertations
Arctic microbial systems continue to get attention today as our understanding regarding their structure and function in a changing system is paramount to C feedbacks with warming and changes in precipitation. Plant communities and microbial community processes across the Arctic landscape are central to understanding tundra ecosystem processes because environmental conditions and plant community structure drive microbial cycling of soil organic matter. Here, we want to understand how soil microbial respiration, mineralization, biomass, and community composition are linked to three Alaskan tundra plant communities, namely Shrub, Tussock, and Sedge tundra and the seasonal variability in this system. A total of …
Composition And Bioavailability Of Effluent Dissolved Organic Nitrogen, Quinn Nicole Roberts
Composition And Bioavailability Of Effluent Dissolved Organic Nitrogen, Quinn Nicole Roberts
Dissertations, Theses, and Masters Projects
Cultural eutrophication, the overproduction of phytoplankton biomass in response to increased nutrient inputs directly associated with human activities, is a major threat to the health of Chesapeake Bay. Strict regulations, which require a reduction in nutrient loading from all sources, have been a key component to restoration efforts. Water reclamation facilities (WRFs), which discharge effluent containing nitrogen (N) and other nutrients into receiving waters, have implemented upgrades in an effort to comply with regulations. These improvements have decreased the concentration of highly labile dissolved inorganic N (DIN), leaving behind significant concentrations of dissolved organic N (DON) whose bioavailability, and therefore …
Determination Of Iron-Reducing Bacterial Activities In Lake Sediments., Alexandra Kahn
Determination Of Iron-Reducing Bacterial Activities In Lake Sediments., Alexandra Kahn
Williams Honors College, Honors Research Projects
Algal blooms are growing rampantly in lacustrine systems due to an increase of phosphorus, a nutrient for algae. Phosphorus is being released into the environment causing overpopulation and eutrophication which damages the ecosystem. The availability of phosphorus is affected by the solubility of iron which is regulated through iron reducing and iron oxidizing bacteria. Phosphorus adheres to insoluble Fe(III), which prohibits algae from utilizing it, while phosphorus does not attach to soluble Fe(II) and therefore it remains available to algae. The purpose of this study was to determine how bacteria influence iron solubility and what are the ideal environmental conditions …
Comparison Of Fe(Iii) Reduction Rates By Iron-Reducing Bacteria Within Sub Muros Samples From Quadrilátero Ferrífero Iron-Ore Caves, Brazil, Summer Ellis
Williams Honors College, Honors Research Projects
Previous research investigating the speleogenesis of iron-ore caves (IOC) in the Quadrilátero Ferrífero, or “Iron Quadrangle,” of Brazil suggests that microbial iron reduction and subsequent dissolution of the surrounding Fe(III) rich rocks is responsible for cave formation. A soft intra-wall substance (sub muros) containing iron-reducing bacteria (FeRB) was discovered underneath the durable crusts of cave walls. The goal of the study was to determine if reduction rates were comparable between sub muros samples, while additionally observing how different electron donors affect microbial iron reduction. To do this, I compared Fe(III) reduction rates between sub muros samples collected from …