Physical Sciences and Mathematics Commons^™

Mycorrhizal Roots In A Temperate Forest Take Up Organic Nitrogen From 13c- And 15n-Labeled Organic Matter, Matthew A. Vadeboncoeur, Andrew P. Ouimette, Erik A. Hobbie

Earth Systems Research Center

Background and Aims

The importance of the uptake of nitrogen in organic form by plants and mycorrhizal fungi has been demonstrated in various ecosystems including temperate forests. However, in previous experiments, isotopically labeled amino acids were often added to soils in concentrations that may be higher than those normally available to roots and mycorrhizal hyphae in situ, and these high concentrations could contribute to exaggerated uptake.

Methods

We used an experimental approach in which we added ¹³C-labeled and ¹⁵N-labeled whole cells to root-ingrowth cores, allowing proteolytic enzymes to release labeled organic nitrogen at a natural rate, as …

Changes In Litter Quality Caused By Simulated Nitrogen Deposition Reinforce The N-Induced Suppression Of Litter Decay, Linda T. A. Van Diepen, Serita D. Frey, Christopher M. Sthultz, Eric W. Morrison, Rakesh Minocha, Anne Pringle

Faculty Publications

Rates of nitrogen (N) deposition are increasing in industrialized and rapidly developing nations. Simulated N deposition suppresses plant litter decay rates, in particular for low quality (high lignin) litter. Litter quality is a primary driver of litter decomposition; however, it is not clear how changes in litter quality caused by long-term ecosystem exposure to chronic N additions interact with altered soil N-availability to influence litter decay dynamics. To document the effects of simulated N deposition on litter quality, we conducted a meta-analysis of available litter nutrient data from simulated N deposition experiments in temperate forests. To directly test whether changes …

Representing Life In The Earth System With Soil Microbial Functional Traits In The Mimics Model, William R. Wieder, A. Stuart Grandy, Cynthia M. Kallenbach, P. G. Taylor, G. B. Bonan

Faculty Publications

Projecting biogeochemical responses to global environmental change requires multi-scaled perspectives that consider organismal diversity, ecosystem processes, and global fluxes. However, microbes, the drivers of soil organic matter decomposition and stabilization, remain notably absent from models used to project carbon (C) cycle–climate feedbacks. We used a microbial trait-based soil C model with two physiologically distinct microbial communities, and evaluate how this model represents soil C storage and response to perturbations. Drawing from the application of functional traits used to model other ecosystems, we incorporate copiotrophic and oligotrophic microbial functional groups in the MIcrobial-MIneral Carbon Stabilization (MIMICS) model; these functional groups are …

Crop Rotational Diversity Enhances Belowground Communities And Functions In An Agroecosystem, L. K. Tiemann, A. Stuart Grandy, E. E. Atkinson, E. Marin-Spiotta, M. D. Mcdaniel

Faculty Publications

Biodiversity loss, an important consequence of agricultural intensification, can lead to reductions in agroecosystem functions and services. Increasing crop diversity through rotation may alleviate these negative consequences by restoring positive aboveground–belowground interactions. Positive impacts of aboveground biodiversity on belowground communities and processes have primarily been observed in natural systems. Here, we test for the effects of increased diversity in an agroecosystem, where plant diversity is increased over time through crop rotation. As crop diversity increased from one to five species, distinct soil microbial communities were related to increases in soil aggregation, organic carbon, total nitrogen, microbial activity and decreases in …

The Promise And Peril Of Intensive-Site-Based Ecological Research: Insights From The Hubbard Brook Ecosystem Study, Timothy J. Fahey, Pamela H. Templer, Bruce T. Anderson, John J. Battles, John L. Campbell, Charles T. Driscoll, Anthony R. Fusco, Mark B. Green, Karim-Aly S. Karrim, Nicholas L. Rodenhouse, Lindsay E. Rustad, Paul G. Schaberg, Matthew A. Vadeboncoeur

Earth Systems Research Center

Abstract.

Ecological research is increasingly concentrated at particular locations or sites. This trend reflects a variety of advantages of intensive, site-based research, but also raises important questions about the nature of such spatially delimited research: how well does site based research represent broader areas, and does it constrain scientific discovery?We provide an overview of these issues with a particular focus on one prominent intensive research site: the Hubbard Brook Experimental Forest (HBEF), New Hampshire, USA. Among the key features of intensive sites are: long-term, archived data sets that provide a context for new discoveries and the elucidation of ecological mechanisms; …

Split-Beam Echosounder Observations Of Natural Methane Seep Variability In The Northern Gulf Of Mexico, Kevin W. Jerram, Thomas C. Weber, Jonathan Beaudoin

Center for Coastal and Ocean Mapping

A method for positioning and characterizing plumes of bubbles from marine gas seeps using an 18 kHz scientific split-beam echo sounder (SBES) was developed and applied to acoustic observations of plumes of presumed methane gas bubbles originating at approximately 1400 m depth in the northern Gulf of Mexico. A total of 161 plume observations from 27 repeat surveys were grouped by proximity into 35 clusters of gas vent positions on the seafloor. Profiles of acoustic target strength per vertical meter of plume height were calculated with compensation for both the SBES beam pattern and the geometry of plume ensonification. These …

Long-Term Forest Soil Warming Alters Microbial Communities In Temperate Forest Soils, Kristen M. Deangelis, Grace Pold, Begum D. Topcuoglu, Linda T. A. Van Diepen, Rebecca M. Varney, Jeffrey L. Blanchard, Jerry Melillo, Serita D. Frey

Faculty Publications

Soil microbes are major drivers of soil carbon cycling, yet we lack an understanding of how climate warming will affect microbial communities. Three ongoing field studies at the Harvard Forest Long-term Ecological Research (LTER) site (Petersham, MA) have warmed soils 5°C above ambient temperatures for 5, 8, and 20 years. We used this chronosequence to test the hypothesis that soil microbial communities have changed in response to chronic warming. Bacterial community composition was studied using Illumina sequencing of the 16S ribosomal RNA gene, and bacterial and fungal abundance were assessed using quantitative PCR. Only the 20-year warmed site exhibited significant …

A Pan-Arctic Synthesis Of Ch4 And Co2 Production From Anoxic Soil Incubations, Claire C. Treat, Susan M. Natali, Jessica G. Ernakovich, Colleen M. Iversen, Massimo Lupascu, Anthony David Mcguire, Richard J. Norby, Taniya Roy Chowdhury, Andreas Richter, Hana Santruckova, Christina Schadel, Edward A. G. Schuur, Victoria L. Sloan, Merritt R. Turestsky, Mark P. Waldrop

Faculty Publications

Permafrost thaw can alter the soil environment through changes in soil moisture, frequently resulting in soil saturation, a shift to anaerobic decomposition, and changes in the plant community. These changes, along with thawing of previously frozen organic material, can alter the form and magnitude of greenhouse gas production from permafrost ecosystems. We synthesized existing methane (CH4) and carbon dioxide (CO2) production measurements from anaerobic incubations of boreal and tundra soils from the geographic permafrost region to evaluate large-scale controls of anaerobic CO2 and CH4 production and compare the relative importance of landscape-level factors (e.g., vegetation type and landscape position), soil …

Arctic Ocean Bathymetry: A Necessary Geospatial Framework, Martin Jakobsson, Larry A. Mayer, Dave Monahan

Center for Coastal and Ocean Mapping

Most ocean science relies on a geospatial infrastructure that is built from bathymetry data collected from ships underway, archived, and converted into maps and digital grids. Bathymetry, the depth of the seafloor, besides having vital importance to geology and navigation, is a fundamental element in studies of deep water circulation, tides, tsunami forecasting, upwelling, fishing resources, wave action, sediment transport, environmental change, and slope stability, as well as in site selection for platforms, cables, and pipelines, waste disposal, and mineral extraction. Recent developments in multibeam sonar mapping have so dramatically increased the resolution with which the seafloor can be portrayed …

Fine-Scale Plant Species Identification In A Poor Fen And Integration Of Techniques And Instrumentation In A Classroom Setting, Dylan Schiff

Honors Theses and Capstones

Refining carbon flux measurements in the carbon cycle is an ongoing challenge. This study attempted to identify plant species in Sallie’s Fen, a nutrient-poor fen in Barrington, New Hampshire, at a fine scale in order to better model and understand carbon exchange between plants and the atmosphere in this type of ecosystem. A protocol for estimating percent cover of species in plots via ground measurements was developed. The next stage of this project was to compare these measurements with measurements derived from spectral images using ImageJ computer software. Statistical tests of the ground measurement data revealed that patterns of seasonal …