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Articles 1 - 4 of 4
Full-Text Articles in Physical Sciences and Mathematics
Neuro And Hepatic Toxicological Profile Of (S)-2,4-Diaminobutanoic Acid In Embryonic, Adolescent And Adult Zebrafish, Rosa-Maria Ferraiuolo, Daniel Meister, Dominique Leckie, Jonathan Franke, Lisa A. Porter, John F. Trant
Neuro And Hepatic Toxicological Profile Of (S)-2,4-Diaminobutanoic Acid In Embryonic, Adolescent And Adult Zebrafish, Rosa-Maria Ferraiuolo, Daniel Meister, Dominique Leckie, Jonathan Franke, Lisa A. Porter, John F. Trant
Chemistry and Biochemistry Publications
(S)-2,4-Diaminobutanoic acid (DABA) is a noncanonical amino acid often co-produced by cyanobacteria along with β-N-methylamino-l-alanine (BMAA) in algal blooms. Although BMAA is a well-established neurotoxin, the toxicity of DABA remains unclear. As part of our development of biocompatible materials, we wish to make use of DABA as both a building block and as the end-product of enzymatically-induced depolymerization; however, if it is toxic at very low concentrations, this would not be possible. We examined the toxicity of DABA using both in vivo embryonic and adult zebrafish models. At higher sub-lethal concentrations (700 µM), the fish demonstrated early …
Protocol Measuring Horizontal Gene Transfer From Algae To Non-Photosynthetic Organisms, James Weifu Lee
Protocol Measuring Horizontal Gene Transfer From Algae To Non-Photosynthetic Organisms, James Weifu Lee
Chemistry & Biochemistry Faculty Publications
Horizontal gene transfer (HGT) is a natural process for an organism to transfer genetic material to another organism that is a completely different species, for example, from a blue-green alga to a non-photosynthetic bacterium. The phenomenon of HGT is not only of an interest to the science of molecular genetics and biology, but also to the biosafety issue of genetic engineering. The novel protocol reported here for the first time teaches how to measure HGT from a genetically engineered (GE) blue-green alga (gene donor) to wild-type E. coli (recipient). This novel protocol can be used to measure HGT frequency for …
The Characterization Of A New Metabolite From A Trichodesmium Bloom, Kelly M. Mcmanus
The Characterization Of A New Metabolite From A Trichodesmium Bloom, Kelly M. Mcmanus
Senior Honors Projects
Our laboratory has been investigating blooms of Trichodesmium, a genus of ecologically important, nitrogen-fixing cyanobacteria, collected from Padre Island in the Gulf of Mexico. Trichodesmium species are an underexplored biological source of cyanobacteria – a taxa that has been shown to produce chemically diverse secondary metabolites. With our focus on the isolation and structure characterization of new bioactive marine natural products, our research group has discovered over 25 new-to-science compounds over the past three years from these blooms. UV and mass spectrometry-guided isolation of Trichodesmium chromatography fractions were utilized to isolate a new metabolite. Isolation of this metabolite was …
The Export Flux Of Particulate Organic Carbon Derived From 210po/210pb Disequilibria Along The North Atlantic Geotraces Ga01 Transect: Geovide Cruise, Yi Tang, Nolwenn Lemaitre, Maxi Castrillejo, Montserrat Roca-Martí, Pere Masqué, Gillian Stewart
The Export Flux Of Particulate Organic Carbon Derived From 210po/210pb Disequilibria Along The North Atlantic Geotraces Ga01 Transect: Geovide Cruise, Yi Tang, Nolwenn Lemaitre, Maxi Castrillejo, Montserrat Roca-Martí, Pere Masqué, Gillian Stewart
Research outputs 2014 to 2021
The disequilibrium between 210Po activity and 210Pb activity in seawater samples was determined along the GEOTRACES GA01 transect in the North Atlantic during the GEOVIDE cruise (May-June 2014). A steady-state model was used to quantify vertical export of particulate 210Po. Vertical advection was incorporated into one version of the model using time-averaged vertical velocity, which had substantial variance. This resulted in large uncertainties for the 210Po export flux in this model, suggesting that those calculations of 210Po export fluxes should be used with great care. Despite the large uncertainties, there is no question that the deficits of 210Po in the …