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Electrochemical Enzyme-Linked Immunosorbent Assay (E-Elisa) For Parasitic Nematode: Ostertagia Ostertagi (Brown Stomach Worm) Infections In Dairy Cattle, Baljit Singh, Evangelia Flampouri, Eithne Dempsey
Electrochemical Enzyme-Linked Immunosorbent Assay (E-Elisa) For Parasitic Nematode: Ostertagia Ostertagi (Brown Stomach Worm) Infections In Dairy Cattle, Baljit Singh, Evangelia Flampouri, Eithne Dempsey
Articles
A sensitive electrochemical immunoassay (e-ELISA) has been developed for the detection of the gastrointestinal parasitic nematode Ostertagia ostertagi (brown stomach worm) in infected and control serum samples. An antigen-indirect immunoassay format was employed to detect the presence of O. ostertagi antibodies, coupled with an anti-species monoclonal horseradish peroxidase (HRP) conjugate. ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) and TMB (3,3',5,5'-tetramethylbenzidine/hydrogen peroxide) were investigated as both chromogenic visualising reagents for optical ELISA and electroactive substrates for electrochemical ELISA in the HRP catalysed oxidation reaction. Coulometry was applied for the detection of O. ostertagi antibodies (via TMB electrochemistry) and compared with the commercial optical ELISA (ABTS …