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Full-Text Articles in Physical Sciences and Mathematics
A Comparative Investigation Of Mammalian Serine Transhydroxymethylases, Sanha Panichajakul
A Comparative Investigation Of Mammalian Serine Transhydroxymethylases, Sanha Panichajakul
Theses and Dissertations
Serine transhydroxymethylase was purified one thousand-fold from bovine brain and one hundred and twenty-fold from bovine liver. Both enzymes were yellow due to the presence of the coenzyme pyridoxal 5'-phosphate. Polyacrylamide gel electrophoresis of the liver enzyme showed one major band containing 95% of the protein, while in the case of the brain enzyme two major bands of nearly equal intensity were observable. The specific activity of the crude liver enzyme was about sixty times higher than that of brain. The liver enzyme was also more heat stable than the brain enzyme. The purified enzymes exhibited L-allothreonine and L-threonine aldolase …
Studies On The Characterization And Activiation By Soluble Factor Of A Sodium-Activated, Magnesium-Dependent, Adenosinetriphosphatase In Rat Cerebral Cortex, Alan B. Forrest
University of the Pacific Theses and Dissertations
This study presented evidence for the existence of a membrane-bound, sodium-activated ATPase which does not require potassium ions for its activity. Furthermore, the data suggest the presence of a soluble factor which activates this enzyme and may play a regulatory role in sodium transport.