Open Access. Powered by Scholars. Published by Universities.®
Physical Sciences and Mathematics Commons™
Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 5 of 5
Full-Text Articles in Physical Sciences and Mathematics
Quantitative Footprinting Analysis Of The Chromomycin A 3 - D N A Interaction, Allison Stankus, Jerry Goodisman, James C. Dabrowiak
Quantitative Footprinting Analysis Of The Chromomycin A 3 - D N A Interaction, Allison Stankus, Jerry Goodisman, James C. Dabrowiak
Chemistry - All Scholarship
Chromomycin A3 (CHR) binding to the duplex d(CAAGTCTGGCCATCAGTC)- d(GACTGATGGCCAGACTTG) has been studied using quantitative footprinting methods. Previous NMR studies indicated CHR binds as a dimer in the minor groove. Analysis of autoradiographic spot intensities derived from DNase I cleavage of the 18-mer in the presence of various amounts of CHR revealed that the drug binds as a dimer to the sequence 5’-TGGCCA-3’, 3’-ACCGGT-5’ in the 18-mer with a binding constant of (2.7 f 1.4) X lo7 M-l. Footprinting and fluorescence data indicate that the dimerization constant for the drug in solution is -lo5 M-l. Since it has been suggested that …
Determination Of Arsenic In A Nickel Alloy By Flow Injection Hydride Generation Atomic Absorption Spectrometry, Julian Tyson, C P. Hanna, S G. Offley
Determination Of Arsenic In A Nickel Alloy By Flow Injection Hydride Generation Atomic Absorption Spectrometry, Julian Tyson, C P. Hanna, S G. Offley
Chemistry Department Faculty Publication Series
The development of a method for the direct determination of trace arsenic quantities in nickel alloy digests, by flow injection hydride generation atomic absorption spectrometry, is described. An optimization study of the manifold and chemical parameters produced system performance, in terms of tolerance of the nickel matrix and sensitivity, such that matrix removal and pre-reduction of As(V) to As (111) prior to arsine generation were eliminated. Full recovery of the As(V) signal from a solution containing 5 ng m1- 1 in the presence of 60 µg mJ- 1 nickel was obtained. Validation of the method was achieved by analyzing a …
Putting The Chemistry Back Into Analytical Chemistry, Julian Tyson
Putting The Chemistry Back Into Analytical Chemistry, Julian Tyson
Chemistry Department Faculty Publication Series
Aspects of the current status of and research in analytical chemistry are briefly discussed and the need for cost effective analytical procedures is emphasized. The present and future of a number of aspects of flow-injection analysis are considered. These include the basic theory, the kinetic features, the control features, time-based methodology, and the coupling of sample pretreatment with instrumentation. Several aspects of this latter topic are considered with particular reference to the flow-injection atomic spectrometry combination. Problems of kinetic mismatch between chemistry, manifold residence, and instrument operation are discussed and some possible solutions proposed including the use of closed loop …
Closed-Loop Recirculating Manifold For Matrix Isolation In Flow Injection Flame Atomic Absorption Spectrometry. Analysis Of Silver Electrolysis Solutions, Ebenezer Debrah, Julian Tyson, Mw Hinds
Closed-Loop Recirculating Manifold For Matrix Isolation In Flow Injection Flame Atomic Absorption Spectrometry. Analysis Of Silver Electrolysis Solutions, Ebenezer Debrah, Julian Tyson, Mw Hinds
Chemistry Department Faculty Publication Series
Two flow injection procedures have been investigated for the determination of some elements in silver electrolysis solutions, for which the problem of the formation of silver acetylide needs to be addressed. A single line manifold was found to give acceptable results for limited time periods, but for prolonged operation it was necessary to remove the silver. This was achieved with a recirculating reactor in which the silver was precipitated as the chloride and retained on a filter of nylon fibers. Good recoveries of copper, iron, nickel and zinc from solutions containing up to 100 g/l. silver were obtained with over …
Flow Injection Spectrochemical Analysis At The Winter Conference, Julian Tyson
Flow Injection Spectrochemical Analysis At The Winter Conference, Julian Tyson
Chemistry Department Faculty Publication Series
No abstract provided.