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Full-Text Articles in Physical Sciences and Mathematics
Synthesis And Evaluation Of Symmetrical Biphenyltetrols As Aggregation Inhibitors For Alzheimer’S Amyloid-Β Peptide, Sarah L. Wicks, James M. Hanna, Robin Lammi
Synthesis And Evaluation Of Symmetrical Biphenyltetrols As Aggregation Inhibitors For Alzheimer’S Amyloid-Β Peptide, Sarah L. Wicks, James M. Hanna, Robin Lammi
The Winthrop McNair Research Bulletin
Inhibiting the aggregation of amyloid-beta peptide (Aβ) is one therapeutic target for the prevention and treatment of Alzheimer’s disease. We have previously demonstrated that biphenyl-3,3′,4,4′-tetrol (3,4-BPT) effectively abrogates Aβ aggregation at stoichiometric concentrations. To investigate molecular architecture and determine how the positioning of the hydroxyl hydrogen-bond donors impacts inhibitor efficacy, we have synthesized four additional symmetrical biphenyltetrols (2,3-, 2,4- 2,5- and 3,5-BPT). We have evaluated these inhibitors by means of Congo red and Thioflavin T dye-binding assays to monitor Aβ aggregation as a function of time and to determine inhibitor IC50 values for reducing equilibrium levels of aggregation. 2,3- and …
Purification And Characterization Of Nickel Uptake Regulator (Nur) And Single Nur Mutants From Streptomyces Coelicolor, Denise Peppers, Nick Grossoehme
Purification And Characterization Of Nickel Uptake Regulator (Nur) And Single Nur Mutants From Streptomyces Coelicolor, Denise Peppers, Nick Grossoehme
The Winthrop McNair Research Bulletin
The Nickel Uptake Regulator (NUR) is a metalloregulatory protein found in the microorganism Streptomyces coelicolor. S.coelicolor is a gram-positive bacterium that plays an important role in antibiotic production. NUR is responsible for the uptake of nickel and the regulation of the enzyme Superoxide Dismutase (SOD) within S.coelicolor. NUR regulates Fe-and Ni-SODs through direct and indirect transcription processes, respectively. The goal of this research is to purify and characterize the metal and DNA binding affinities in Wild Type (WT) NUR. There are two metal binding sites within NUR that is believed to contribute to the function of this protein. These sites …
The Reaction Of O-Silylated Cyanohydrin Anions With Epoxides As An Alternative For The Enantio- And Diastereoselective Preparation Of Aldols, Diamond Rose Melendez, Aaron Hartel
The Reaction Of O-Silylated Cyanohydrin Anions With Epoxides As An Alternative For The Enantio- And Diastereoselective Preparation Of Aldols, Diamond Rose Melendez, Aaron Hartel
The Winthrop McNair Research Bulletin
The aldol addition is one of the most important and most utilized carbon-carbon bond forming reactions in chemical synthesis. This reaction, between an aldehyde or ketone and a second, enolized aldehyde or ketone, results in the formation of a -hydroxycarbonyl (often referred to as an “aldol product” or “aldol”). Modern variations of the aldol reaction have allowed for enantio- and diastereoselectivity in the reaction; however, many of these methods have undesirable drawbacks such as the use of expensive chiral auxiliaries. The chiral auxiliaries require additional synthesis steps for their introduction and removal and cannot be completely recovered after the reaction …
Cloning And Characterization Of Nickel Uptake Regulator Nur Mutants From Streptomyces Coelicolor, Olivia Manley, Nick E. Grossoehme
Cloning And Characterization Of Nickel Uptake Regulator Nur Mutants From Streptomyces Coelicolor, Olivia Manley, Nick E. Grossoehme
The Winthrop McNair Research Bulletin
Sufficient concentrations of metal within a cell are required for proper cellular function; however, metals become toxic at very high concentrations. Therefore, it is important for an organism to have a mechanism for maintaining metal homeostasis within its cells. Streptomyces coelicolor, a soil-dwelling bacterium important in the production of antibiotics, utilizes the nickel uptake regulator (NUR) to maintain nickel homeostasis and oxidative response. NUR functions as a transcriptional repressor that responds to changing cytosolic concentrations of Ni2+. Previous research describes two key metal-binding sites per NUR monomer. Our research seeks to analyze how changes to these binding …