Open Access. Powered by Scholars. Published by Universities.®
Physical Sciences and Mathematics Commons™
Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- Nanoparticle (3)
- Cytochrome c (2)
- Anhydrous proton conduction (1)
- Chiroptical (1)
- Drug delivery (1)
-
- Dynamics (1)
- Electron transfer (1)
- Electrospray Ionization (ESI) (1)
- Enzyme-Substrate Complex (1)
- Fe(III) reduction (1)
- Fuel cells (1)
- Geobacter sulfurreducens (1)
- Mass Spectrometry (1)
- Nanomedicine (1)
- Polymer electrolyte membrane (1)
- Protein Architecture (1)
- Protein Conformation (1)
- Protein Polymer Conjugation (1)
- Protein recognition (1)
- Protein refolding (1)
- Recognition (1)
- Single Molecule Sepectroscopy (1)
- Structure-property relation (1)
- Surface recognition (1)
- Thermodynamics (1)
- Transfection (1)
- Triazole (1)
Articles 1 - 7 of 7
Full-Text Articles in Physical Sciences and Mathematics
Tailoring The Surface-Coating Of Gold Nanoparticles For Bio-Applications, Partha S. Ghosh
Tailoring The Surface-Coating Of Gold Nanoparticles For Bio-Applications, Partha S. Ghosh
Open Access Dissertations
Functionalized gold nanoparticles (AuNPs) provide an excellent scaffold for numerous biological applications. In these systems, the gold core imparts stability to the assembly, while the monolayer allows tuning of surface characteristics such as charge and hydrophobicity. The nano-scale size and tunable surface properties have made them an ideal candidate for manipulating protein-protein/protein-nucleic acid interactions, and delivery of therapeutics. In this thesis work, it has been demonstrated how the surface coating plays an important role in achieving a desired goal. Using organic synthesis as a tool, the monolayer was tailored to afford useful particles with biocompatibility and the ability to respond …
Intermolecular Electron Transfer Reactivity And Dynamics Of Cytochrome C – Nanoparticle Adducts, Adrienne M. Carver
Intermolecular Electron Transfer Reactivity And Dynamics Of Cytochrome C – Nanoparticle Adducts, Adrienne M. Carver
Open Access Dissertations
Interprotein electron transfer (ET) is crucial for natural energy conversion and a fundamental reaction in the pursuit of understanding the broader problem of proteinprotein interactions and reactivity. Simplifying the complicated nature of these natural systems has driven development of biomimetic approaches. Functionalized gold nanoparticles offer simplified, tunable surfaces that can serve as a proxy to study the reactivity and dynamics of proteins. Amino-acid functionalized gold nanoparticles (Au-TX) served as a complementary partner to cytochrome c (Cyt c) and catalyzed its ET reactivity without altering the native structure. Redox mediator and EPR experiments confirmed that the redox potential and coordination environment …
High Temperature Proton Conducting Materials And Fluorescent-Labeled Polymers For Sensor Applications, Surangkhana Martwiset
High Temperature Proton Conducting Materials And Fluorescent-Labeled Polymers For Sensor Applications, Surangkhana Martwiset
Open Access Dissertations
The majority of this dissertation focuses on proton conducting materials that could be used at high operating temperatures. Higher operating temperatures are desirable as they will increase fuel cell efficiency, reduce cost, and simplify the heat management system. The factors governing proton conduction including segmental mobility, protogenic group identity, and charge carrier density were investigated on a variety of polymers containing 1H-1,2,3-triazole moieties. Proton conductivity measurements were made using AC impedance spectroscopy. Random copolymers and terpolymers of triazole-containing acrylates and poly(ethylene glycol)methyl ether acrylate (PEGMEA) have been synthesized. Conductivity increased with increasing degree of PEG incorporation until reaching a maximum …
Investigation Of Fe(Iii) Reduction In Geobacter Sulfurreducens Characterization Of Outer Surface Associated Electron Transfer Components, Xenlei Qian
Open Access Dissertations
Outer membrane cytochromes OmcB and OmcS of Geobacter sulfurreducens are two important components of the respiratory chain for extracellular Fe(III) reduction. OmcS is a loosely bound cell surface protein involved in the reduction of insoluble Fe(III). OmcB is an outer membrane protein and required for insoluble and soluble Fe(III) reduction. The objective of this study was to understand better the mechanism of dissimilatory Fe(III) reduction, focusing on the cell surface proteins by further localization, identification of protein-protein interactions, and biochemical characterization of OmcB and OmcS. OmcB was found to be surface-exposed but embedded in the outer membrane because mild protease …
Single Molecule Chiroptical Spectroscopy: Fluorescence Excitation Circular Dichroism And Circular Polarized Luminescence Of Bridged Triarylamine Helicenes, Ruthanne Hassey Paradise
Single Molecule Chiroptical Spectroscopy: Fluorescence Excitation Circular Dichroism And Circular Polarized Luminescence Of Bridged Triarylamine Helicenes, Ruthanne Hassey Paradise
Open Access Dissertations
In this thesis, I describe the first exploratory experimental efforts probing light-matter interactions of chiral systems at the single molecule level. The dissymmetric single molecule chiroptical response in both excitation and emission polarization has been studied for different diastereomeric forms of bridged triarylamine helicenes. Fluorescence excitation circular dichroism (FECD), measuring the dissymmetric absorption with respect to excitation polarization, reports on the response to excitation polarization. The magnitude and distribution of chiroptical single molecule responses suggest both surface and orientation effects play a significant role. Computational modeling done to calculate the dissymmetry for specific orientations supports orientational dependence. Using a defocused …
Developing Novel Electrospray Ionization Mass Spectrometry (Esi Ms) Techniques To Study Higher Order Structure And Interaction Of Biopolymers, Agya K. Frimpong
Developing Novel Electrospray Ionization Mass Spectrometry (Esi Ms) Techniques To Study Higher Order Structure And Interaction Of Biopolymers, Agya K. Frimpong
Open Access Dissertations
Mass spectrometry has enjoyed enormous popularity over the years for studying biological systems. The theme of this dissertation was to develop and use mass spectrometry based tools to solve five biologically oriented problems associated with protein architecture and extend the utility of these tools to study protein polymer conjugation. The first problem involved elucidating the false negatives of how proteins with few basic residues, forms highly charged ions in electrospray ionization mass spectrometry (ESI MS). This study showed that the unfolding of polypeptide chains in solution leads to the emergence of highly charged protein ions in ESI MS mass spectra, …
Engineering The Nanoparticle Surface For Protein Recognition And Applications, Mrinmoy De
Engineering The Nanoparticle Surface For Protein Recognition And Applications, Mrinmoy De
Open Access Dissertations
Proteins and nanoparticles (NPs) provide a promising platform for supramolecular interaction. We are currently exploring both fundamental and applied aspects of this interaction. On the fundamental side, we have fabricated a series of water-soluble anionic and cationic NPs to interact with cationic and anionic proteins respectively. A Varity of studies such as, activity assay, fluorescence titration, isothermal titration calorimetry etc. were carried out to quantify the binding properties of these functional NPs with those proteins. Those studies reveal the prospect of tuning the affinity between the nanoparticles and proteins by the surface modification. On the application side, we have used …