Physical Sciences and Mathematics Commons^™

Roles Of Serine 101, Histidine 310 And Valine 464 In The Reaction Catalyzed By Choline Oxidase From Arthrobacter Globiformis, Steffan Finnegan

Chemistry Dissertations

The enzymatic oxidation of choline to glycine betaine is of interest because organisms accumulate glycine betaine intracellularly in response to stress conditions, as such it is of potential interest for the genetic engineering of crops that do not naturally possess efficient pathways for the synthesis of glycine betaine, and for the potential development of drugs that target the glycine betaine biosynthetic pathway in human pathogens. To date, one of the best characterized enzymes belonging to this pathway is the flavin-dependent choline oxidase from Arthrobacter globiformis. In this enzyme, choline oxidation proceeds through two reductive half-reactions and two oxidative half-reactions. In …