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Full-Text Articles in Physical Sciences and Mathematics
Methods For Increasing The Electron Richness Of Iron Tricarbonyl Diimine Complexes, Francesca Lombardi, Joseph Maher, Sean Peloquin
Methods For Increasing The Electron Richness Of Iron Tricarbonyl Diimine Complexes, Francesca Lombardi, Joseph Maher, Sean Peloquin
Chemistry & Biochemistry Student Scholarship
Francesca Lombardi ’24, Major: Chemistry
Joseph Maher ’25, Major: Chemistry
Sean Peloquin ’26, Major: Biochemistry
Faculty Mentor: Dr. Maria Carroll, Chemistry and Biochemistry
The goal of our research is to synthesize new iron complexes that can reduce carbon dioxide. Increasing the electron richness of iron tricarbonyl diimine complexes, which have been synthesized previously in the Carroll research group, will increase the likelihood of a reaction with carbon dioxide occurring. We have taken two approaches to changing the electron density in the complexes. First, we reacted the complex Fe((CF3)2ArDABMe)(CO)¬3 with the reducing agent potassium on graphite, in order to reduce either …
Kinetic Analysis Of Glu115ala Cytosolic Human Malate Dehydrogenase Mutation Shows A Change In Activity On Non-Native Substrate Phenylpyruvate, Kailey Paar
Chemistry & Biochemistry Student Scholarship
The cytosolic human enzyme, malate dehydrogenase (MDH1), is believed to have a significant effect on the proliferation of cancerous cells. This enzyme oxidizes NADH to NAD+ as it converts oxaloacetate (OAA) to malate. This oxidation of NADH provides the needed NAD+ to cancer cells, which require NAD+ as they upregulate glycolysis in aerobic conditions. Targeting MDH1 can reveal new therapeutic treatments for cancer by preventing the conversion of OAA to malate and depleting NAD+ stores in cancer cells, which can be done by mutating residues in the enzyme and assessing the change in activity. Here we show the replacement of …
Metal Complexes Of Redox Active Ligands, Alexandra Chaparro, Parker Keller
Metal Complexes Of Redox Active Ligands, Alexandra Chaparro, Parker Keller
Chemistry & Biochemistry Student Scholarship
Alexandra Chaparro ’22, Major: Biochemistry
Parker Keller ’24, Major: Chemistry
Mentor: Dr. Maria Carroll, Chemistry and Biochemistry
Our research focuses on synthesizing and studying the properties of metal complexes that contain redox active ligands. Ligands are molecules or ions that can bind to a metal ion, and this particular class of ligands is interesting because they can either accept or lose electrons. We synthesized zinc complexes, in order to measure the reduction potentials of the ligands, which provide information about the ease with which they accept electrons. We then synthesized iron complexes and determined their structures. These complexes are potentially …
A Damaged Reputation: Nuclear Depictions In Entertainment Media, Alexander Cannon
A Damaged Reputation: Nuclear Depictions In Entertainment Media, Alexander Cannon
Chemistry & Biochemistry Student Scholarship
This paper, originally written for Dr. Adamus Liotta in an honors chemistry class, explores the possibility of a significant influence over public common sense found in the depictions of entertainment media. Through careful analysis and interpretation of Godzilla, The Simpsons, Fallout, and Call of Duty, this paper finds a synergy throughout this diverse range of audience, medium and historical origin, which culminates in an effect much greater than the sum of its parts. This effect frames nuclear science, of any kind, in a hyperbolically catastrophic and horrific light, reinforcing an excessively cautious, pessimistic public perspective on nuclear technology …
Towards The Development Of Low-Cost And Easily-Deployable Sensing Platforms For Phosphate, Maureen Pontarelli, Thomas Koch
Towards The Development Of Low-Cost And Easily-Deployable Sensing Platforms For Phosphate, Maureen Pontarelli, Thomas Koch
Chemistry & Biochemistry Student Scholarship
Maureen Pontarelli ’20
Major: Chemistry
Thomas Koch '20
Major: Biochemistry
Faculty Mentor: Dr. John Breen, Chemistry and Biochemistry
Exploration Of K2 Mycobacteriophages With Bred, Colby Agostino, Olivia Schmitt
Exploration Of K2 Mycobacteriophages With Bred, Colby Agostino, Olivia Schmitt
Chemistry & Biochemistry Student Scholarship
Colby Agostino ’22
Major: Biochemistry and Computer Science
Olivia Schmitt ’22
Major: Biochemistry
Faculty Mentor: Dr. Kathleen A. Cornely, Chemistry and Biochemistry
ZoeJ and Marcoliusprime are mycobacteriophage capable of infecting tuberculosis causing bacteria via the lytic and lysogenic cycles. The lytic cycle is the form of viral replication that results in cell death. Lysogeny involves phage DNA being integrated with the DNA of the host cell and preserved through binary fission. However, the bacteria causing tuberculosis do not die in the lysogenic phase. This research project will involve creating mutants of ZoeJ and Marcoliusprime that will not contain the genes …
Mycobacteriophage Morphology As A Diagnostic For Cluster Assignment, Ethan Dionne
Mycobacteriophage Morphology As A Diagnostic For Cluster Assignment, Ethan Dionne
Chemistry & Biochemistry Student Scholarship
Major: Biochemistry
Faculty Mentor: Dr. Kathleen A. Cornely, Chemistry and Biochemistry
Phages are viral bodies that infect bacterial hosts, and have shown promising applications as alternatives to antibiotics for the treatment of bacterial infections. This project examines the morphology of siphoviridae mycobacteriophage, which have long, flexible, non-contractile tails as well as the characteristic head, called a capsid. Using electron microscopy photos of sequenced phages, tail length and capsid diameter were measured and compared to further characterize morphological relationships between genetically distinct phages. The data presented has the potential to work as a diagnostic tool to classify unsequenced phages to genetically …
Surface Mutation Thr34his Facilitates Purification Of Haemophilus Influenza Carbonic Anhydrase Via Metal Affinity Chromatography, Caroline Foley, Dr. Kathleen Cornely
Surface Mutation Thr34his Facilitates Purification Of Haemophilus Influenza Carbonic Anhydrase Via Metal Affinity Chromatography, Caroline Foley, Dr. Kathleen Cornely
Chemistry & Biochemistry Student Scholarship
In order to pursue Haemophilus influenza carbonic anhydrase (HICA) as a potential drug target, easy and efficient purification methods must be developed. While immobilized metal affinity chromatography (IMAC) may be used, complications with polyhistidine tags is a concern. Inspired by the endogenous metal affinity of Escherichia coli β-carbonic anhydrase (ECCA), we suggest that the generation of histidine clusters on HICA’s surface will facilitate its purification by metal affinity chromatography without the potential interference of His-tags. Here we investigate the Thr34His mutation as a method to generate metal affinity in HICA. Since Thr34His is located only 5.3 Å away from His32, …
E56h + E59h Mutation Affects Binding Of The Recombinant Haemphilus Influenza Carbonic Anhydrase To A Ni-Nta Column, Eric D. Lebel, Kathleen Cornely
E56h + E59h Mutation Affects Binding Of The Recombinant Haemphilus Influenza Carbonic Anhydrase To A Ni-Nta Column, Eric D. Lebel, Kathleen Cornely
Chemistry & Biochemistry Student Scholarship
The Haemophilus influenzae carbonic anhy- drase (HICA) is important in converting carbon dioxide to bicarbonate in bacteria. Endogenous cellular proteins, like Escherichia coli carbonic anhydrase (ECCA), have been observed to bind to a Ni-NTA column, which can be used as a means of protein purification. The possibility ex- ists that proteins that do not normally bind to Ni-NTA, like HICA, can be engineered using site directed mutagenesis to introduce histidine resi- dues that would give the protein the capability to bind, allowing for a one-step purification method. Site-directed mutagenesis was used to introduce the double mutation of E56H + E59H …
Mutation Of Surface Histidines In Haemophilus Influenzae Carbonic Anhydrase To Enable Purification By Metal Affinity Chromatography, Shelby E. Scola, Kathleen Cornely
Mutation Of Surface Histidines In Haemophilus Influenzae Carbonic Anhydrase To Enable Purification By Metal Affinity Chromatography, Shelby E. Scola, Kathleen Cornely
Chemistry & Biochemistry Student Scholarship
No abstract provided.