Open Access. Powered by Scholars. Published by Universities.®

Physical Sciences and Mathematics Commons™

Open Access. Powered by Scholars. Published by Universities.®

Chemistry

University of Nebraska - Lincoln

Series

2001

Articles 1 - 24 of 24

Full-Text Articles in Physical Sciences and Mathematics

The Biosynthetic Gene Cluster For The Anticancer Drug Bleomycin From Streptomyces Verticillus Atcc15003 As A Model For Hybrid Peptide–Polyketide Natural Product Biosynthesis, B. Shen, Liangcheng Du, C. Sanchez, D. J. Edwards, M. Chen, J. M. Murrell Dec 2001

The Biosynthetic Gene Cluster For The Anticancer Drug Bleomycin From Streptomyces Verticillus Atcc15003 As A Model For Hybrid Peptide–Polyketide Natural Product Biosynthesis, B. Shen, Liangcheng Du, C. Sanchez, D. J. Edwards, M. Chen, J. M. Murrell

Liangcheng Du Publications

The hybrid peptide–polyketide backbone of bleomycin (BLM) is assembled by the BLM megasynthetase that consists of both nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) modules. BlmIX/BlmVIII/BlmVII constitute a natural hybrid NRPS/PKS/NRPS system, serving as a model for both hybrid NRPS/PKS and PKS/NRPS systems. Sequence analysis and functional comparison of domains and modules of BlmIX/BlmVIII/BlmVII with those of nonhybrid NRPS and PKS systems suggest that (1) the same catalytic sites appear to be conserved in both hybrid NRPS–PKS and nonhybrid NRPS or PKS systems, with the exception of the KS domains in the hybrid NRPS/PKS systems that are unique; (2) …

Go to article

Direct Imaging Of Human Swi/Snf-Remodeled Mono- And Polynucleosomes By Atomic Force Microscopy Employing Carbon Nanotube Tips, Gavin R. Schnitzler, Chin Li Cheung, Jason H. Hafner, Andrew J. Saurin, Robert E. Kingston, Charles M. Lieber Dec 2001

Direct Imaging Of Human Swi/Snf-Remodeled Mono- And Polynucleosomes By Atomic Force Microscopy Employing Carbon Nanotube Tips, Gavin R. Schnitzler, Chin Li Cheung, Jason H. Hafner, Andrew J. Saurin, Robert E. Kingston, Charles M. Lieber

Barry Chin Li Cheung Publications

Chromatin-remodeling complexes alter chromatin structure to facilitate, or in some cases repress, gene expression. Recent studies have suggested two potential pathways by which such regulation might occur. In the first, the remodeling complex repositions nucleosomes along DNA to open or occlude regulatory sites. In the second, the remodeling complex creates an altered dimeric form of the nucleosome that has altered accessibility to transcription factors. The extent of translational repositioning, the structure of the remodeled dimer, and the presence of dimers on remodeled polynucleosomes have been difficult to gauge by biochemical assays. To address these questions, ultrahigh-resolution carbon nanotube tip atomic …

Go to article

Magnetic Ordering In An Organic Polymer, Andrzej Rajca, Jirawat Wongsriratanakul, Suchada Rajca Nov 2001

Magnetic Ordering In An Organic Polymer, Andrzej Rajca, Jirawat Wongsriratanakul, Suchada Rajca

Andrzej Rajca Publications

We describe preparation and magnetic properties of an organic p-conjugated polymer with very large magnetic moment and magnetic order at low temperatures. The polymer is designed with a large density of cross-links and alternating connectivity of radical modules with unequal spin quantum numbers (S), macrocyclic S = 2 and, cross-linking S = ½ modules, which permits large net S values for either ferromagnetic or antiferromagnetic exchange couplings between the modules. In the highly cross-linked polymer, an effective magnetic moment corresponding to an average S of about 5000 and slow reorientation of the magnetization by a small magnetic field …

Go to article

Monte Carlo Simulation Of Homogeneous Binary Vapor–Liquid Nucleation: Mutual Enhancement Of Nucleation In A Partially Miscible System, S. Yoo, K.J. Oh, Xiao Cheng Zeng Nov 2001

Monte Carlo Simulation Of Homogeneous Binary Vapor–Liquid Nucleation: Mutual Enhancement Of Nucleation In A Partially Miscible System, S. Yoo, K.J. Oh, Xiao Cheng Zeng

Xiao Cheng Zeng Publications

Gibbs free energy of ij-cluster formation ΔGij is obtained via the relation nij=Ne-ΔGij /kBT, where N is the number of molecules in the system, T is temperature, and kB is the Boltzmann constant. The saddle point on the free energy surface ΔGij versus i and j provides a measure of the activation barrier to nucleation ΔG *. A statistical mechanical formula of ΔGij is derived based on the physical-cluster theory of nucleation, and from which a molecular interpretation of ΔGij …

Go to article

Energetics And Cooperativity In Three-Center Hydrogen Bonding Interactions. Ii. Intramolecular Hydrogen Bonding Systems, Ruben D. Parra, Bing Gong, Xiao Cheng Zeng Oct 2001

Energetics And Cooperativity In Three-Center Hydrogen Bonding Interactions. Ii. Intramolecular Hydrogen Bonding Systems, Ruben D. Parra, Bing Gong, Xiao Cheng Zeng

Xiao Cheng Zeng Publications

High level ab initio calculations have been carried out on a number of intramolecular two- and three-center H-bonding structures containing the amide motif, NHC=O. These structures serve as model systems for probing the stability of intramolecular three-center H bonds of the type A1HA2. Particular attention is given to cooperative effects in the three-center H-bonding structures. A systematic assessment of cooperativity for intramolecular H-bonding systems is presented.

Go to article

Energetics And Cooperativity In Three-Center Hydrogen Bonding Interactions. I. Diacetamide-X Dimers .X=Hcn, Ch3Oh., Ruben D. Parra, Makoto Furukawa, Bing Gong, Xiao Cheng Zeng Oct 2001

Energetics And Cooperativity In Three-Center Hydrogen Bonding Interactions. I. Diacetamide-X Dimers .X=Hcn, Ch3Oh., Ruben D. Parra, Makoto Furukawa, Bing Gong, Xiao Cheng Zeng

Xiao Cheng Zeng Publications

High level ab initio calculations are carried out on diacetamide–X (DA–X) dimers, X=HCN, CH3OH. The dimers are used as model systems to investigate the energetics and cooperative phenomomena in intermolecular three-center hydrogen-bond (H-bond) interactions relative to two-center H-bond interactions. The trans–trans conformer of diacetamide is chosen as a suitable model for intermolecular three-center H bonding where one H atom is interacting with two acceptor atoms. The proton–acceptor atoms are rigidly held in the same molecule. For both model systems, it is found that the calculated interaction energy per H bond is appreciably smaller in the three-center than in …

Go to article

Formation Of Ordered Ice Nanotubes Inside Carbon Nanotubes, Kenichiro Koga, G. T. Gao, Hideki Tanaka, Xiao Cheng Zeng Aug 2001

Formation Of Ordered Ice Nanotubes Inside Carbon Nanotubes, Kenichiro Koga, G. T. Gao, Hideki Tanaka, Xiao Cheng Zeng

Xiao Cheng Zeng Publications

Following their discovery, carbon nanotubes have attracted interest not only for their unusual electrical and me-chanical properties, but also because their hollow interior can serve as a nanometre-sized capillary, mould, or template in material fabrication. The ability to en¬capsulate a material in a nanotube also offers new possibili¬ties for investigating dimensionally confined phase transi¬tions . Particularly intriguing is the conjecture that matter within the narrow confines of a carbon nanotube might ex¬hibit a solid–liquid critical point beyond which the distinc¬tion between solid and liquid phases disappears. This unusual feature, which cannot occur in bulk material, would allow for the direct …

Go to article

Structural And Functional Imaging With Carbon Nanotube Afm Probes, J. H. Hafner, Chin Li Cheung, A. T. Woolley, C. M. Lieber Jul 2001

Structural And Functional Imaging With Carbon Nanotube Afm Probes, J. H. Hafner, Chin Li Cheung, A. T. Woolley, C. M. Lieber

Barry Chin Li Cheung Publications

Atomic force microscopy (AFM) has great potential as a tool for structural biology, a field in which there is increasing demand to characterize larger and more complex biomolecular systems. However, the poorly characterized silicon and silicon nitride probe tips currently employed in AFM limit its biological applications. Carbon nanotubes represent ideal AFM tip materials due to their small diameter, high aspect ratio, large Young’s modulus, mechanical robustness, well-defined structure, and unique chemical properties. Nanotube probes were first fabricated by manual assembly, but more recent methods based on chemical vapor deposition provide higher resolution probes and are geared towards mass production, …

Go to article

Cyp83b1 Is The Oxime-Metabolizing Enzyme In The Glucosinolate Pathway In Arabidopsis, Carsten Horslev Hansen, Liangcheng Du, Peter Naur, Carl Erik Olsen, Kristian B. Axelsen, Alastair J. Hick, John A. Pickett, Barbara Ann Halkier Jul 2001

Cyp83b1 Is The Oxime-Metabolizing Enzyme In The Glucosinolate Pathway In Arabidopsis, Carsten Horslev Hansen, Liangcheng Du, Peter Naur, Carl Erik Olsen, Kristian B. Axelsen, Alastair J. Hick, John A. Pickett, Barbara Ann Halkier

Liangcheng Du Publications

CYP83B1 from Arabidopsis thaliana has been identified as the oxime-metabolizing enzyme in the biosynthetic pathway of glucosinolates. Biosynthetically active microsomes isolated from Sinapis alba converted p-hydroxyphenylacetaldoxime and cysteine into S-alkylated p-hydroxyphenylacetothiohydroximate, S-(p-hydroxyphenylacetohydroximoyl)-L-cysteine, the next proposed intermediate in the glucosinolate pathway. The production was shown to be dependent on a cytochrome P450 monooxygenase. We searched the genome of A. thaliana for homologues of CYP71E1 (P450ox), the only known oxime-metabolizing enzyme in the biosynthetic pathway of the evolutionarily related cyanogenic glucosides. By a combined use of bioinformatics, published expression data, and knock-out phenotypes, we identified the …

Go to article

Nature Of Oxygen At Rocksalt And Spinel Oxide Surfaces, Marjorie Langell, J.G. Kim, D. L. Pugmire, W. Mccarroll Jul 2001

Nature Of Oxygen At Rocksalt And Spinel Oxide Surfaces, Marjorie Langell, J.G. Kim, D. L. Pugmire, W. Mccarroll

Marjorie A. Langell Publications

The chemical environment of oxygen in cobalt-containing metal oxides with compositions M xM′( x – 1) O and M xM′(3x – 1) O4 (M,M′ = Mn,Ni,Co) has been studied by Auger, x-ray and ultraviolet photoelectron, and high resolution electron energy loss spectroscopies. While there is a single type of lattice oxygen in the bulk structure of simple rocksalt and spinel oxides, the nature of oxygen at the surface of the spinel oxides is considerably more complex. Photoemission from core oxygen states in these materials often shows multiple peaks and satellite structure which have been …

Go to article

Quorum Sensing In The Dimorphic Fungus Candida Albicans Is Mediated By Farnesol, Jacob M. Hornby, Ellen C. Jensen, Amber C. Lisec, Joseph J. Tasto, Brandon Jahnke, Richard Shoemaker, Patrick Dussault, Kenneth W. Nickerson Jul 2001

Quorum Sensing In The Dimorphic Fungus Candida Albicans Is Mediated By Farnesol, Jacob M. Hornby, Ellen C. Jensen, Amber C. Lisec, Joseph J. Tasto, Brandon Jahnke, Richard Shoemaker, Patrick Dussault, Kenneth W. Nickerson

Patrick Dussault Publications

The inoculum size effect in the dimorphic fungus Candida albicans results from production of an extracellular quorum-sensing molecule (QSM). This molecule prevents mycelial development in both a growth morphology assay and a differentiation assay using three chemically distinct triggers for germ tube formation (GTF): L-proline, N-acetylglucosamine, and serum (either pig or fetal bovine). In all cases, the presence of QSM prevents the yeast-to-mycelium conversion, resulting in actively budding yeasts without influencing cellular growth rates. QSM exhibits general cross-reactivity within C. albicans in that supernatants from strain A72 are active on five other strains of C. albicans and vice versa. …

Go to article

Molecular Dynamics Simulation Of Supersaturated Vapor Nucleation In Slit Pore. Ii. Thermostatted Atomic-Wall Model, K.T. Kholmurodov, K. Yasuoka, Xiao Cheng Zeng Jun 2001

Molecular Dynamics Simulation Of Supersaturated Vapor Nucleation In Slit Pore. Ii. Thermostatted Atomic-Wall Model, K.T. Kholmurodov, K. Yasuoka, Xiao Cheng Zeng

Xiao Cheng Zeng Publications

Molecular dynamics simulations of nucleation of a supersaturated Lennard-Jones vapor in slit nanopores are carried out. In this study we extend a previous work [K. Yasuoka, G. T. Gao, and X. C. Zeng, J. Chem. Phys. 112, 4279 (2000)] in that the walls of the slit are treated as actual atomic walls serving as both the confining solid surfaces and a thermostat. The walls are fixed in place in a fcc lattice structure and wall atoms are subjected to a stiff biharmonic potential thereby bounded to lattice sites. The two walls of the slit have an identical surface [fcc (100)], …

Go to article

Nucleation Of Water And Methanol Droplets On Cations And Anions: The Sign Preference, K.J. Oh, G.T. Gao, Xiao Cheng Zeng May 2001

Nucleation Of Water And Methanol Droplets On Cations And Anions: The Sign Preference, K.J. Oh, G.T. Gao, Xiao Cheng Zeng

Xiao Cheng Zeng Publications

The barrier height to cation- and anion-induced nucleation to produce water and methanol droplets are calculated by means of an umbrella-sampling Monte Carlo method. The computer simulation corroborates the century-old finding of Wilson that the anion is a better nucleator to produce water droplets than the cation having the same magnitude of charge, even without the presence of external electric field. The simulation also shows that the cation is a better nucleator to produce methanol droplets than the anion.

Go to article

Energy Gaps In “Metallic” Single-Walled Carbon Nanotubes, Min Ouyang, Jin-Lin Huang, Chin Li Cheung, Charles Lieber Apr 2001

Energy Gaps In “Metallic” Single-Walled Carbon Nanotubes, Min Ouyang, Jin-Lin Huang, Chin Li Cheung, Charles Lieber

Barry Chin Li Cheung Publications

Metallic single-walled carbon nanotubes have been proposed to be good one-dimensional conductors. However, the finite curvature of the graphene sheet that forms the nanotubes and the broken symmetry due to the local environment may modify their electronic properties. We used low-temperature atomically resolved scanning tunneling microscopy to investigate zigzag and armchair nanotubes, both thought to be metallic. “Metallic” zigzag nanotubes were found to have energy gaps with magnitudes that depend inversely on the square of the tube radius, whereas isolated armchair tubes do not have energy gaps. Additionally, armchair nanotubes packed in bundles have pseudogaps, which exhibit an inverse dependence …

Go to article

A Greek Cross Dodecaphenylene: Sparteine-Mediated Asymmetric Synthesis Of Chiral D2-Symmetric Π-Conjugated Tetra-O-Phenylenes, Andrzej Rajca, Hua Wang, Pavel Bolshov, Suchada Rajca Apr 2001

A Greek Cross Dodecaphenylene: Sparteine-Mediated Asymmetric Synthesis Of Chiral D2-Symmetric Π-Conjugated Tetra-O-Phenylenes, Andrzej Rajca, Hua Wang, Pavel Bolshov, Suchada Rajca

Andrzej Rajca Publications

The asymmetric synthesis of a chiral, nonracemic π-conjugated system with D2 point group of symmetry, dodecaphenylene 4 is described. In the key step, (−)-sparteine- and Cu(II)-mediated oxidation of 2,2′-dilithio-1,1′-biaryls in ether gives the corresponding dimers, tetra-o-phenylenes, in 80% isolated yields and 50–60% ee’s. X-Ray crystallography confirms the structure of rac-4 and its molecular shape of a Greek cross. The torsional angles between the benzene rings in the tetra-o-phenylene core of rac-4 are in the 56.5–71.0° range. However, CD and UV spectra of 4 in CH2Cl2 are consistent with significant conjugation between the …

Go to article

Dependence Of 13C Nmr Chemical Shifts On Conformations Of Rna Nucleosides And Nucleotides, Mohsen Ebrahimi, Paolo Rossi, Christopher Rogers, Gerard S. Harbison Apr 2001

Dependence Of 13C Nmr Chemical Shifts On Conformations Of Rna Nucleosides And Nucleotides, Mohsen Ebrahimi, Paolo Rossi, Christopher Rogers, Gerard S. Harbison

Gerard Harbison Publications

Cross-polarization magic-angle spinning solid-state NMR spectroscopy has been used to investigate the dependence of 13C sugar chemical shifts on specific conformational parameters of a variety of ribonucleotides and ribonucleosides. Solid-state NMR is a valuable tool for nucleoside and nucleotide structural studies since it provides the means to acquire spectra that correspond to single conformations, as opposed to 13C solutionNMRmethods. The distinct effects of sugar puckering on the C1′, C4′, and C5′ resonances of C2′ endo (S type) and C3′ endo (N type) furanoid conformations allow us to separate them into two groups. Further analysis of each group reveals …

Go to article

Solution Structure Of B. Subtilis Acyl Carrier Protein, Guang-Yi Xu, Amy Tam, Laura Lin, Jeffrey Hixon, Christian C. Fritz, Robert Powers Apr 2001

Solution Structure Of B. Subtilis Acyl Carrier Protein, Guang-Yi Xu, Amy Tam, Laura Lin, Jeffrey Hixon, Christian C. Fritz, Robert Powers

Robert Powers Publications

Background: Acyl carrier protein (ACP) is a fundamental component of fatty acid biosynthesis in which the fatty acid chain is elongated by the fatty acid synthetase system while attached to the 4’-phosphopantetheine prosthetic group (4’- PP) of ACP. Activation of ACP is mediated by holo-acyl carrier protein synthase (ACPS) when ACPS transfers the 4’-PP moiety from coenzyme A (CoA) to Ser36 of apo-ACP. Both ACP and ACPS have been identified as essential for E. coli viability and potential targets for development of antibiotics.

Results: The solution structure of B. subtilis ACP (9 kDa) has been determined using two-dimensional and three-dimensional …

Go to article

Calculation Of 13C Chemical Shifts In Rna Nucleosides: Structure-13C Chemical Shift Relationships, Paolo Rossi, Gerard S. Harbison Feb 2001

Calculation Of 13C Chemical Shifts In Rna Nucleosides: Structure-13C Chemical Shift Relationships, Paolo Rossi, Gerard S. Harbison

Gerard Harbison Publications

Isotropic 13C chemical shifts of the ribose sugar in model RNA nucleosides are calculated using SCF and DFT-GIAO ab initio methods for different combinations of ribose sugar pucker, exocyclic torsion angle, and glycosidic torsion angle. Idealized conformations were obtained using structures that were fully optimized by ab initio DFT methods starting with averaged parameters from a collection of crystallographic data. Solid-state coordinates of accurate crystal or neutron diffraction structures were also examined directly without optimization. The resulting 13C chemical shifts for the two sets of calculations are then compared. The GIAO-DFT method overestimates the shifts by an average …

Go to article

Effect Of Carrier-Gas Pressure On Barrier To Nucleation: Monte Carlo Simulation Of Water/Nitrogen System, K.J. Oh, Xiao Cheng Zeng Feb 2001

Effect Of Carrier-Gas Pressure On Barrier To Nucleation: Monte Carlo Simulation Of Water/Nitrogen System, K.J. Oh, Xiao Cheng Zeng

Xiao Cheng Zeng Publications

Carrier gases are used in most nucleation experiments for releasing the latent heat generated during vapor condensation. In the analysis of experimental data it is often assumed that the carrier gas is inert and would not participate in the nucleation process of the target gas. Several recent nucleation experiments show that the influence of carrier gases to nucleation rate is not negligible under certain conditions. To gain more insight into the carrier-gas effect, we carry out Monte Carlo simulation to compute the free energy of formation of water clusters in the presence of a nitrogen carrier gas. At fixed temperature …

Go to article

Adsorption And Decomposition Of Nickelocene On Ag(100): A High-Resolution Electron Energy Loss Spectroscopy And Temperature Programmed Desorption Study, D. L. Pugmire, C. M. Woodbridge, N. M. Boag, Marjorie Langell Feb 2001

Adsorption And Decomposition Of Nickelocene On Ag(100): A High-Resolution Electron Energy Loss Spectroscopy And Temperature Programmed Desorption Study, D. L. Pugmire, C. M. Woodbridge, N. M. Boag, Marjorie Langell

Marjorie A. Langell Publications

Nickelocene adsorption and decomposition on the Ag(100) surface were studied with temperature programmed desorption and high resolution electron energy loss spectroscopy. At monolayer coverages on the relatively inert Ag(100) surface at 175 K, nickelocene physisorbs molecularly, with its molecular axis perpendicular to the surface plane. Nickelocene begins decomposing to adsorbed cyclopentadienyl and nickel at 225 K. Molecular desorption is only observed from multilayer material, at 210 K, or from the first monolayer if adjacent surface sites for decomposition are not available. The cyclopentadienyl decomposes through disproportionation to cyclopentadiene, which desorbs, and adsorbed nickel and carbon fragments on the Ag(100) surface …

Go to article

Effects Of External Electric Field On The Interfacial Properties Of Weakly Dipolar Fluid, V.B. Warshavsky, T.V. Bykov, Xiao Cheng Zeng Feb 2001

Effects Of External Electric Field On The Interfacial Properties Of Weakly Dipolar Fluid, V.B. Warshavsky, T.V. Bykov, Xiao Cheng Zeng

Xiao Cheng Zeng Publications

In the framework of modified mean-field density-functional theory, effects of a uniform electric field on the interfacial properties of a model dipolar fluid [Teixeira and Telo da Gama, J. Phys.: Condens. Matter 3, 111 (1991)] are studied. Both density and orientational order-parameter profiles of the planar vapor-liquid interface are obtained as a function of the field strength. For the dipolar fluids with reduced dipole moment μ0*0*E*/T*!1) can shift the surface tension by few percent. We also find that the electric field actually reduces the thermodynamical surface tension but enhances the mechanical surface …

Go to article

Atomically Resolved Single-Walled Carbon Nanotube Intramolecular Junctions, Min Ouyang, Jin-Lin Huang, Chin Li Cheung, Charles M. Lieber Jan 2001

Atomically Resolved Single-Walled Carbon Nanotube Intramolecular Junctions, Min Ouyang, Jin-Lin Huang, Chin Li Cheung, Charles M. Lieber

Barry Chin Li Cheung Publications

Intramolecular junctions in single-walled carbon nanotubes are potentially ideal structures for building robust, molecular-scale electronics but have only been studied theoretically at the atomic level. Scanning tunneling microscopy was used to determine the atomic structure and electronic properties of such junctions in single-walled nanotube samples. Metal-semiconductor junctions are found to exhibit an electronically sharp interface without localized junction states, whereas a more diffuse interface and low-energy states are found in metal-metal junctions. Tight-binding calculations for models based on observed atomic structures show good agreement with spectroscopy and provide insight into the topological defects forming intramolecular junctions. These studies have important …

Go to article

Multiple Separations Facilitate Identification Of Protein Variants By Mass Spectrometry, Zhongli Zhang, David L. Smith, Jean B. Smith Jan 2001

Multiple Separations Facilitate Identification Of Protein Variants By Mass Spectrometry, Zhongli Zhang, David L. Smith, Jean B. Smith

Chemistry Department: Faculty Publications

Identification of variant proteins from complex biological samples promises to contribute much to our understanding of the etiology of pathological states. Characterization of variants, due either to genetic mutations in protein sequences or to post-translational modifications, is considerably more difficult than the simple protein identifications typical of most current proteomic investigations. Identification of a few peptides by database retrieval is not adequate when the goal is to have a complete understanding of the modifications of the protein. Although one advantage of mass spectrometry is its ability to obtain specific responses to several components, the complexity of biological samples is often …

Go to article

Preliminary X-Ray Diffraction Studies Of The Transcriptional Inhibitory Antibody Fab41.4, Johanna Mazlo, Robyn L. Stanfield, Ian A. Wilson, Steven H. Hinrichs, John J. Stezowski Jan 2001

Preliminary X-Ray Diffraction Studies Of The Transcriptional Inhibitory Antibody Fab41.4, Johanna Mazlo, Robyn L. Stanfield, Ian A. Wilson, Steven H. Hinrichs, John J. Stezowski

Chemistry Department: Faculty Publications

The binding of transcription factor ATF-1 to DNA contributes to gene expression and regulation of cell growth. Antibody Mab41.4, raised against ATF-1, and its derivatives Fab41.4 and scFv41.4 inhibit specific DNA binding in vitro and induce apoptotic death of tumor cells in vivo. Structural studies of Fab41.4 were performed to gain insight into the mechanism of action of this potentially therapeutic antibody. The optimal conditions for crystallization of Fab41.4 were determined. Crystals were needle-like in appearance, displayed C2 space-group symmetry and diffracted to a resolution of 1.6 Å. The unit-cell parameters were determined to be a = 186.64, b = …

Go to article